Human TREM-2 Antibody

  (8 citations)
(2 Reviews)
    
Datasheet / CoA / SDS
Product Details
Citations (8)
FAQs
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  • Species Reactivity
    Human
  • Specificity
    Detects human TREM‑2 in direct ELISAs and Western blots. In direct ELISAs, less than 10% cross-reactivity with recombinant mouse TREM‑2b is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TREM-2
    His19-Ser174
    Accession # Q9NZC2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Product Datasheets

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Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    Human peripheral blood monocytes
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human TREM‑2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TREM‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1828) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TREM‑2 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
TREM‑2 in Human Dendritic Cells. TREM‑2 was detected in immersion fixed immature human dendritic cells using Goat Anti-Human TREM‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1828) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
    Reconstitution Buffer Available
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TREM-2
TREM-2 is a single Ig domain receptor expressed by cells of myeloid lineage. It is a pattern recognition receptor that binds anionic ligands. TREM-2 associates with the adapter protein DAP12 to deliver an activating signal that plays a role in both innate and adaptive immune responses.
  • Long Name:
    Triggering Receptor Expressed on Myeloid Cells 2
  • Entrez Gene IDs:
    54209 (Human)
  • Alternate Names:
    TREM2; TREM-2; Trem2a; Trem2b; Trem2c; TREM-2triggering receptor expressed on myeloid cells 2a; Triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

8 Citations: Showing 1 - 8
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Species
Applications
Sample Type
  1. A split-luciferase complementation, real-time reporting assay enables monitoring of the disease-associated transmembrane protein TREM2 in live cells
    Authors: MM Varnum, KA Clayton, A Yoshii-Kit, G Yonemoto, L Koro, S Ikezu, T Ikezu
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  2. TREM2 shedding by cleavage at the H157-S158 bond is accelerated for the Alzheimer's disease-associated H157Y variant
    Authors: P Thornton, J Sevalle, MJ Deery, G Fraser, Y Zhou, S Ståhl, EH Franssen, RB Dodd, S Qamar, B Gomez Pere, LS Nicol, S Eketjäll, J Revell, C Jones, A Billinton, PH St George-, I Chessell, DC Crowther
    EMBO Mol Med, 2017;0(0):.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  3. An Alzheimer-associated TREM2 variant occurs at the ADAM cleavage site and affects shedding and phagocytic function
    Authors: K Schlepckow, G Kleinberge, A Fukumori, R Feederle, SF Lichtentha, H Steiner, C Haass
    EMBO Mol Med, 2017;0(0):.
    Species: Human
    Sample Type: Cell Lystaes
    Application: WB
  4. Intracellular trafficking of TREM2 is regulated by presenilin 1
    Authors: Y Zhao, X Li, T Huang, LL Jiang, Z Tan, M Zhang, IH Cheng, X Wang, G Bu, YW Zhang, Q Wang, H Xu
    Exp. Mol. Med., 2017;49(12):e405.
    Species: Human
    Sample Type: Protein
    Application: WB
  5. TREM-2 promotes acquired cholesteatoma-induced bone destruction by modulating TLR4 signaling pathway and osteoclasts activation
    Sci Rep, 2016;6(0):38761.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Fresh
  6. Inhibition of RANK expression and osteoclastogenesis by TLRs and IFN-gamma in human osteoclast precursors.
    Authors: Ji JD, Park-Min KH, Shen Z, Fajardo RJ, Goldring SR, McHugh KP, Ivashkiv LB
    J. Immunol., 2009;183(11):7223-33.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  7. IL-10 suppresses calcium-mediated costimulation of receptor activator NF-kappa B signaling during human osteoclast differentiation by inhibiting TREM-2 expression.
    Authors: Park-Min KH, Ji JD, Antoniv T, Reid AC, Silver RB, Humphrey MB, Nakamura M, Ivashkiv LB
    J. Immunol., 2009;183(4):2444-55.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  8. TREM-2 binds to lipooligosaccharides of Neisseria gonorrhoeae and is expressed on reproductive tract epithelial cells.
    Authors: Quan DN, Cooper MD, Potter JL, Roberts MH, Cheng H, Jarvis GA
    Mucosal Immunol, 2008;1(3):229-38.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC

FAQs

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Reconstitution Buffer 1 (PBS)

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Chicken Anti-Goat IgG Biotinylated Antibody

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Average Rating: 5 (Based on 2 reviews)

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We have 2 review tested in 1 species: Human.
We have 2 reviews tested in 2 applications: Western Blot, Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human TREM-2 Antibody AF1828 Array AF1828
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Excellent
 WB Human Anonymous 06/08/2017
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Image Details
Western Blot Human TREM-2 Antibody AF1828
Western Blot: Human TREM-2 Antibody [AF1828].

Summary

Application Western Blot
Sample Tested HEK293T human embryonic kidney cell line
Species Human
  Excellent
 Flow Human Anonymous 05/16/2017
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Summary

Application Flow Cytometry
Sample Tested 293T human embryonic kidney cell line
Species Human

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