hh Human TREM-2 Antibody AF1828: R&D Systems

Human TREM-2 Antibody

(5 citations)
(2 Reviews)
  
  • Species Reactivity
    Human
  • Specificity
    Detects human TREM-2 in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant mouse (rm) TREM-2b is observed and less than 1% cross-reactivity with recombinant human (rh) TREM-1, rhTLT-1, rmTREM-L2, rmTREM-3 and rmTREM-4 is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human TREM-2
    His19-Ser174
    Accession # Q9NZC2
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.2 µg/mL
    See below
  • Flow Cytometry
    2.5 µg/106 cells
    Human peripheral blood monocytes
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
  • Immunocytochemistry
    5-15 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human TREM‑2 by Western Blot. Western blot shows lysates of THP‑1 human acute monocytic leukemia cell line. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human TREM‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1828) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for TREM‑2 at approximately 28 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
TREM‑2 in Human Dendritic Cells. TREM‑2 was detected in immersion fixed immature human dendritic cells using Goat Anti-Human TREM‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1828) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: TREM-2
TREM-2 is a single Ig domain receptor expressed by cells of myeloid lineage. It is a pattern recognition receptor that binds anionic ligands. TREM-2 associates with the adapter protein DAP12 to deliver an activating signal that plays a role in both innate and adaptive immune responses.
  • Long Name:
    Triggering Receptor Expressed on Myeloid Cells 2
  • Entrez Gene IDs:
    54209 (Human)
  • Alternate Names:
    TREM2; TREM-2; Trem2a; Trem2b; Trem2c; TREM-2triggering receptor expressed on myeloid cells 2a; Triggering receptor expressed on monocytes 2; triggering receptor expressed on myeloid cells 2
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

5 Citations: Showing 1 - 5
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Species
Applications
Sample Type
  1. A split-luciferase complementation, real-time reporting assay enables monitoring of the disease-associated transmembrane protein TREM2 in live cells
    Authors: MM Varnum, KA Clayton, A Yoshii-Kit, G Yonemoto, L Koro, S Ikezu, T Ikezu
    J. Biol. Chem., 2017;0(0):.
    Species: Human
    Sample Type: Whole Cells
    Application: Functional Assay
  2. TREM-2 promotes acquired cholesteatoma-induced bone destruction by modulating TLR4 signaling pathway and osteoclasts activation
    Sci Rep, 2016;6(0):38761.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC - Fresh
  3. IL-10 suppresses calcium-mediated costimulation of receptor activator NF-kappa B signaling during human osteoclast differentiation by inhibiting TREM-2 expression.
    Authors: Park-Min KH, Ji JD, Antoniv T, Reid AC, Silver RB, Humphrey MB, Nakamura M, Ivashkiv LB
    J. Immunol., 2009;183(4):2444-55.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  4. Inhibition of RANK expression and osteoclastogenesis by TLRs and IFN-gamma in human osteoclast precursors.
    Authors: Ji JD, Park-Min KH, Shen Z, Fajardo RJ, Goldring SR, McHugh KP, Ivashkiv LB
    J. Immunol., 2009;183(11):7223-33.
    Species: Human
    Sample Type: Cell Lysates
    Application: WB
  5. TREM-2 binds to lipooligosaccharides of Neisseria gonorrhoeae and is expressed on reproductive tract epithelial cells.
    Authors: Quan DN, Cooper MD, Potter JL, Roberts MH, Cheng H, Jarvis GA
    Mucosal Immunol, 2008;1(3):229-38.
    Species: Human
    Sample Type: Whole Tissue
    Application: IHC
Cell and Tissue Staining Kits
Description Application Cat# Citations Images  

Anti-Goat HRP-DAB Cell & Tissue Staining Kit

CTS008 13
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Isotype Controls
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Normal Goat IgG Control

Ctrl AB-108-C 191  
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Secondary Antibodies
Description Application Cat# Citations Images  

Goat IgG HRP-conjugated Antibody

WB, Simple Western HAF109 19  
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Goat IgG HRP-conjugated Antibody

WB HAF017 15  
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Goat IgG (H+L) PE-conjugated Antibody

Flow F0107 4  
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Donkey Anti-Goat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL001 9
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Donkey Anti-Goat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL003 5
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Goat IgG (H+L) APC-conjugated Antibody

Flow F0108 6  
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Goat IgG Horseradish Peroxidase-conjugated Antibody

WB HAF019 6  
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Donkey Anti-Goat IgG Antibody

WB AF109 6
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Donkey Anti-Goat IgG Biotinylated Antibody

WB BAF109 3
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Goat IgG VisUCyte HRP Polymer

IHC VC004  
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Goat IgG (H+L) Fluorescein-conjugated Antibody

Flow F0109 2  
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Donkey Anti-Goat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL002
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Rabbit Anti-Goat IgG (H+L) Affinity Purified PAb, X Absorbed

WB, ELISA R-401-C-ABS
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Chicken Anti-Goat IgG Biotinylated Antibody

WB BAF019
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Rabbit Anti-Goat IgG Biotinylated Antibody

WB BAF017
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Donkey Anti-Goat IgG (H+L) PerCP-conjugated Antibody

Flow F0124
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Average Rating: 5 (Based on 2 reviews)

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We have 2 reviews tested in 1 species: Human.
We have 2 reviews tested in 2 applications: Western Blot, Flow Cytometry.

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Images Ratings Applications Species Reviewed By Date Details
Western Blot Human TREM-2 Antibody AF1828
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Excellent
 WB Human Anonymous 06/08/2017
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Western Blot Human TREM-2 Antibody AF1828
Western Blot: Human TREM-2 Antibody [AF1828]

Summary

ApplicationWestern Blot
Sample TestedHEK293T human embryonic kidney cell line
SpeciesHuman
  Excellent
 Flow Human Anonymous 05/16/2017
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Summary

ApplicationFlow Cytometry
Sample Tested293T human embryonic kidney cell line
SpeciesHuman

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