Human Trypsin 1/PRSS1 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human Trypsin 1/PRSS1 in direct ELISAs and Western blots. In direct ELISAs, approximately 50% cross-reactivity with recombinant human (rh) Trypsin 2 and rhTrypsin 3 is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant human Trypsin 1/PRSS1
    Ala16-Ser247
    Accession # P07477
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunohistochemistry
Trypsin 1/PRSS1 in Human Pancreas. Trypsin 1/PRSS1 was detected in immersion fixed paraffin-embedded sections of human pancreas using Mouse Anti-Human Trypsin 1/PRSS1 Monoclonal Antibody (Catalog # MAB3848) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to exocrine cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Trypsin 1/PRSS1

Human Trypsin 1, encoded by the PRSS1 gene, is also known as Cationic Trypsinogen (1). Constituting approximately two-thirds of the total trypsin content in normal pancreatic juice, it is the most abundant trypsin isoform produced by the pancreas. It contains a signal peptide (residues 1‑15), a pro region (residues 16‑23), and a mature chain (residues 24‑247). Trypsin 1 is synthesized in the pancreas and secreted into the duodenum lumen, where it is activated by enterokinase. Its major physiologic function is to digest food and to activate other pro-enzymes (2). Mutations in the PRSS1 gene can cause hereditary pancreatitis (3).

  • References:
    1. Emi, M. et al. (1986) Gene 41:305.
    2. Halfon, S. et al. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) p. 1483, Academic Press, San Diego.
    3. Teich, N. et al. (2006) Hum. Mutat. 27:721.
  • Entrez Gene IDs:
    5644 (Human); 114228 (Mouse); 24691 (Rat)
  • Alternate Names:
    Beta-trypsin; Cationic trypsinogen; digestive zymogen; DKFZp779A0837; EC 3.4.21; EC 3.4.21.4; FLJ57296; MGC120175; MGC149362; nonfunctional trypsin 1; protease, serine, 1 (trypsin 1); PRSS1; PTRYI; Serine protease 1; TRP1; TRY1beta-trypsin; TRY4; Trygn16; TRYP1; Trypsin 1; Trypsin I; trypsin-1; Trypsinogen 1; trypsinogen A
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