Key Product Details

Species Reactivity

Human

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 484404
View all TSHR Primary Antibodies »

Product Specifications

Immunogen

NS0 mouse myeloma cell line transfected with human TSH R
Accession # P16473

Specificity

Detects human TSH R in direct ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human TSHR Antibody

Detection of TSH R antibody in HEK293 Human Cell Line Transfected with Human TSH R and eGFP antibody by Flow Cytometry.

Detection of TSH R in HEK293 Human Cell Line Transfected with Human TSH R and eGFP by Flow Cytometry.

HEK293 human embryonic kidney cell line transfected with either (A) human TSH R or (B) irrelevant transfectants and eGFP was stained with Mouse Anti-Human TSH R Monoclonal Antibody (Catalog # MAB65342) followed by Allophycocyanin-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # F0101B). Quadrant markers were set based on control antibody staining (Catalog # MAB002). View our protocol for Staining Membrane-associated Proteins.

Applications for Human TSHR Antibody

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: HEK293 Human Cell Line Transfected with Human TSH R and eGFP

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: TSHR

Thyroid stimulating hormone receptor (TSH R; also thyrotropin receptor) is an 85 kDa (unglycosylated) member of the G-protein coupled receptor 1 family. Human TSH R is synthesized as a 764 amino acid (aa) precursor that contains a 20 aa signal sequence, a 393 aa extracellular domain (ECD), a 269 aa membrane spanning domain consisting of seven transmembrane segments, and an 82 aa cytoplasmic domain. The ECD contains six leucine-rich repeats (LRR) and six potential sites of N-linked glycosylation. There are two isoforms, long and short, produced by a splicing variant corresponding to aa 253-764 in the long isoform that is missing in the short isoform. Human TSH R is 86-87% aa identical to mouse and rat TSH R. TSH R is the receptor for thyroid stimulating hormone, which plays a central role in controlling thyroid cell metabolism.

Long Name

Thyroid Stimulating Hormone Receptor

Alternate Names

CHNG1, LGR3, TSH R

Entrez Gene IDs

7253 (Human); 22095 (Mouse)

Gene Symbol

TSHR

UniProt

P16473

Additional TSHR Products

Product Documents for Human TSHR Antibody

Certificate of Analysis

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Product Specific Notices for Human TSHR Antibody

For research use only

Citations for Human TSHR Antibody

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