Human Twist-2 Antibody

• Species Reactivity
  Human
• Specificity
  Detects human Twist-2 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human Twist-1 is observed.
• Source
  Polyclonal Sheep IgG
• Purification
  Antigen Affinity-purified
• Immunogen
  E. coli-derived recombinant human Twist-2
  Met1-Ser159
  Accession # Q8WVJ9
• Formulation
  Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
• Label
  Unconjugated

• Immunocytochemistry
  5-15 µg/mL
  See below

Immunocytochemistry click image to view larger

Twist‑2 in A549 Human Cell Line. Twist‑2 was detected in immersion fixed A549 human lung carcinoma cells treated with Recombinant Human TGF-beta 1 (Catalog # 240-B) using Human Twist‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6249) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

• Reconstitution
  Sterile PBS to a final concentration of 0.2 mg/mL.

  Reconstitution Buffer Available
• Shipping
  The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
• Stability & Storage
  Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

• Long Name:
  Twist Related Protein 2 [Drosophila]

• Entrez Gene IDs:
  117581 (Human)

• Alternate Names:
  bHLHa39; bHLHa39twist-related bHLH protein Dermo1; Class A basic helix-loop-helix protein 39; Dermis-expressed protein 1; DERMO1; Dermo-1; DERMO1MGC117334; twist homolog 2 (Drosophila); Twist2; Twist-2; twist-related protein 2

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