Twist‑2 in A549 Human Cell Line. Twist‑2 was detected in immersion fixed A549 human lung carcinoma cells treated with Recombinant Human TGF-beta 1 (Catalog # 240-B) using Human Twist‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6249) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Twist-2 (Twist-related protein 2; also Dermo-1 and bHLHa39) is a 19-20 kDa Class B member of the bHLH transcription factor family of proteins. It is expressed in embryonic mesodermal and ectodermal derivatives, and is widely expressed in adult cells, including preosteoblasts, gastric chief cells, preadipocytes and myeloid progenitor cells. Twist-2 is a transcriptional repressor that heterodimerizes with multiple partners such as E12, SREBP1 and MEF2. Human Twist-2 is 160 amino acids (aa) in length. It contains a bHLH domain (aa 65-122) with an embedded DNA-binding motif. Full length human Twist-2 shares 82% and 99% aa identity with human Twist-1 and mouse Twist-2, respectively.
Twist Related Protein 2 [Drosophila]
Entrez Gene IDs:
bHLHa39; bHLHa39twist-related bHLH protein Dermo1; Class A basic helix-loop-helix protein 39; Dermis-expressed protein 1; DERMO1; Dermo-1; DERMO1MGC117334; twist homolog 2 (Drosophila); Twist2; Twist-2; twist-related protein 2
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