Human Twist-2 Antibody

Catalog # Availability Size / Price Qty
AF6249
AF6249-SP
Twist‑2 in A549 Human Cell Line.
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Human Twist-2 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Twist-2 in direct ELISAs. In direct ELISAs, less than 5% cross-reactivity with recombinant human Twist-1 is observed.
Source
Polyclonal Sheep IgG
Purification
Antigen Affinity-purified
Immunogen
E. coli-derived recombinant human Twist-2
Met1-Ser159
Accession # Q8WVJ9
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Immunocytochemistry
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Example

Immunocytochemistry Twist‑2 in A549 Human Cell Line. View Larger

Twist‑2 in A549 Human Cell Line. Twist‑2 was detected in immersion fixed A549 human lung carcinoma cells treated with Recombinant Human TGF-beta 1 (Catalog # 240-B) using Human Twist‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6249) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Reconstitution Calculator

Reconstitution Calculator

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Preparation and Storage

Reconstitution
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Twist-2

Twist-2 (Twist-related protein 2; also Dermo-1 and bHLHa39) is a 19-20 kDa Class B member of the bHLH transcription factor family of proteins. It is expressed in embryonic mesodermal and ectodermal derivatives, and is widely expressed in adult cells, including preosteoblasts, gastric chief cells, preadipocytes and myeloid progenitor cells. Twist-2 is a transcriptional repressor that heterodimerizes with multiple partners such as E12, SREBP1 and MEF2. Human Twist-2 is 160 amino acids (aa) in length. It contains a bHLH domain (aa 65-122) with an embedded DNA-binding motif. Full length human Twist-2 shares 82% and 99% aa identity with human Twist-1 and mouse Twist-2, respectively.

Long Name
Twist Related Protein 2 [Drosophila]
Entrez Gene IDs
117581 (Human)
Alternate Names
bHLHa39; bHLHa39twist-related bHLH protein Dermo1; Class A basic helix-loop-helix protein 39; Dermis-expressed protein 1; DERMO1; Dermo-1; DERMO1MGC117334; twist homolog 2 (Drosophila); Twist2; Twist-2; twist-related protein 2

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