Human UNC5H4 Antibody
Human UNC5H4 Antibody Summary
Accession # NP_543148
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Caenorhabditis elegans UNC5 (UNC = behaviorally uncoordinated) and its mammalian homologues, UNC5H1-4, UNC5A-D, and rostral cerebellar malformation (RCM), are transmembrane proteins belonging to the immunoglobulin (Ig) superfamily. All UNC5 family members have two Ig and two thrombospondin type 1 domains in their extracellular regions, as well as a conserved ZU-5 domain, a DCC (Deleted in Colorectal Cancer)-binding domain (DB) and a C-terminal death domain (DD) in their cytoplasmic regions (1, 2). Human UNC5H4 cDNA encodes a 948 amino acid (aa) residues type I membrane protein with a putative 14 aa signal peptide and 359 aa extracellular domain. The extracellular domain of human UNC5H4 shares approximately 97% and 66% aa sequence identity with mouse UNC5H4 and human UNC5H3, respectively.
UNC5 family proteins are receptors for the netrin/UNC6 family of secreted axon guidance cues that are laminin-related proteins. Netrin family proteins can act as a chemoattractant for some axons and as a chemorepellent for others. Besides UNC5, netrin family proteins also bind to the DCC family of type I transmembrane receptors and to adenosine A2b receptor, a G protein-coupled seven-transmembrane receptor belonging to the adenosine receptor family (3, 4). In vitro, netrin binding to DCC family receptors in the absence of UNC5 is associated with axon attraction. However, the DCC-mediated attraction to netrin is converted to repulsion by binding of UNC5 to the DCC-netrin complex. In vivo, the mechanisms of netrin-dependent axon attraction and repulsion are more complex and may include UNC5-mediated repulsion that is independent of DCC (1, 5). Besides their roles in axon guidance and neuronal migration, the UNC5 and DCC families also act as dependence receptors and exert pro-apoptotic effects in the absence of netrin (6).
- Hong, K. et al. (1999) Cell 97:927.
- Leonardo, E.D. et al. (1997) Nature 386:833.
- Culotti, J.B. and D.C. Merz (1998) Curr. Opin. Cell Biol. 10:609.
- Corset, V. (2000) Nature 407:747.
- Merz, D.C. (2001) Genetics 158:1071.
- Llambi, F. et al. (2001) EMBO Journal 20:2715.
Citations for Human UNC5H4 Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
Citations: Showing 1 - 3
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Netrin-1 receptor UNC5C cleavage by active &delta-secretase enhances neurodegeneration, promoting Alzheimer's disease pathologies
Authors: G Chen, SS Kang, Z Wang, EH Ahn, Y Xia, X Liu, IM Sandoval, FP Manfredsso, Z Zhang, K Ye
Science Advances, 2021;7(16):.
Sample Types: Tissue Homogenates
Applications: Western Blot
Drebrin-like (Dbnl) Controls Neuronal Migration via Regulating N-Cadherin Expression in the Developing Cerebral Cortex
Authors: S Inoue, K Hayashi, K Fujita, K Tagawa, H Okazawa, KI Kubo, K Nakajima
J. Neurosci., 2019;39(4):678-691.
Sample Types: Whole Tissue
Single-cell RNA sequencing reveals metallothionein heterogeneity during hESC differentiation to definitive endoderm
Authors: J Lu, A Baccei, E Lummertz d, C Guillermie, S McManus, LA Finney, C Zhang, ML Steinhause, H Li, PH Lerou
Stem Cell Res, 2018;28(0):48-55.
Sample Types: Whole Cells
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