Detection of Human Uromodulin by Western Blot.
Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5144) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Uromodulin at approximately 80-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Uromodulin in Human Kidney.
Uromodulin was detected in immersion fixed paraffin-embedded sections of human kidney using 1.7 µg/mL Sheep Anti-Human Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5144) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.
Preparation and Storage
Reconstitute at 0.2 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Uromodulin (also Tamm-Horsfall glycoprotein or THP) is an 85‑95 kDa urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL‑1, TNF-alpha and C1q, activates resting monocytes and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidnay tubules. Human Uromodulin is GPI‑linked. Its proprecursor is 616 amino acids (aa) in length. It contains three EGF-like domains (aa 28‑149), a ZP domain that mediates oligomerization (aa 334‑589) and a cleavable C‑terminal propeptide (aa 615‑640). There are multiple splice variants. One shows a deletion of aa 67‑199, a second shows a nine aa substitution for aa 609‑640, a third shows a Pro substitution for aa 205‑234 and a fourth shows a 66 aa substitution for aa 613‑640. Over aa 25‑614, human Uromodulin is 78% aa identical to mouse Uromodulin.
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