Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Western Blot, Simple Western

Cited:

IF/IHC

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all Uromodulin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Uromodulin isoform 1
Asp25-Ser614
Accession # P07911

Specificity

Detects human Uromodulin in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 25% cross-reactivity with recombinant mouse Uromodulin is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human Uromodulin Antibody

Detection of Human Uromodulin antibody by Western Blot.

Detection of Human Uromodulin by Western Blot.

Western blot shows lysates of human kidney tissue. PVDF membrane was probed with 0.5 µg/mL of Sheep Anti-Human Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5144) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Uromodulin at approximately 80-90 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Detection of Human Uromodulin by Simple WesternTM.

Simple Western shows lysates of Exosome Standards (Human Urine) (NBP2-49840) and human kidney tissue, loaded at 0.5 mg/ml. A specific band was detected for Uromodulin at approximately 149 kDa (as indicated) using 50 µg/mL of Sheep Anti-Human Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5144). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
Uromodulin antibody in Human Kidney by Immunohistochemistry (IHC-P).

Uromodulin in Human Kidney.

Uromodulin was detected in immersion fixed paraffin-embedded sections of human kidney using 1.7 µg/mL Sheep Anti-Human Uromodulin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5144) overnight at 4 °C. Tissue was stained with the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific labeling was localized to epithelial cells in tubules. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human Uromodulin Antibody

Application
Recommended Usage

Immunohistochemistry

2-15 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human kidney

Simple Western

50 µg/mL
Sample: Exosome Standards (Human Urine) (Catalog # NBP2-49840) and human kidney tissue

Western Blot

0.5 µg/mL
Sample: Human kidney tissue

Reviewed Applications

Read 1 review rated 5 using AF5144 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Uromodulin

Uromodulin (also Tamm-Horsfall glycoprotein or THP) is an 85‑95 kDa urinary glycoprotein. It is secreted by renal tubule epithelium, acts as a binding protein for IL‑1, TNF-alpha and C1q, activates resting monocytes and promotes neutrophil phagocytosis. Uromodulin forms high molecular weight oligomers that line the kidnay tubules. Human Uromodulin is GPI‑linked. Its proprecursor is 616 amino acids (aa) in length. It contains three EGF-like domains (aa 28‑149), a ZP domain that mediates oligomerization (aa 334‑589) and a cleavable C‑terminal propeptide (aa 615‑640). There are multiple splice variants. One shows a deletion of aa 67‑199, a second shows a nine aa substitution for aa 609‑640, a third shows a Pro substitution for aa 205‑234 and a fourth shows a 66 aa substitution for aa 613‑640. Over aa 25‑614, human Uromodulin is 78% aa identical to mouse Uromodulin.

Alternate Names

ADMCKD2, FJHN, HNFJ, MCKD2, THGP, THP, UMOD, Uromucoid

Entrez Gene IDs

7369 (Human); 22242 (Mouse); 25128 (Rat)

Gene Symbol

UMOD

UniProt

P07911

Additional Uromodulin Products

Product Documents for Human Uromodulin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Uromodulin Antibody

For research use only

Citations for Human Uromodulin Antibody

Customer Reviews for Human Uromodulin Antibody (1)

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  • Human Uromodulin Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Urine
    Species: Rat
    Verified Customer | Posted 07/06/2018
    Human Uromodulin Antibody AF5144

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Protocols

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