Human Uteroglobin/SCGB1A1 Antibody

Catalog # Availability Size / Price Qty
MAB4218
MAB4218-SP
Human Uteroglobin/SCGB1A1 Antibody in Immunohistochemistry
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Product Details
Citations (1)
FAQs
Supplemental Products
Reviews (4)

Human Uteroglobin/SCGB1A1 Antibody Summary

Species Reactivity
Human
Specificity
Detects human Uteroglobin/SCGB1A1 in direct ELISAs and Western blots.
Source
Monoclonal Rat IgG1 Clone # 394324
Purification
Protein A or G purified from hybridoma culture supernatant
Immunogen
E. coli-derived recombinant human Uteroglobin/SCGB1A1
Glu22-Asn91
Accession # P11684
Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Label
Unconjugated

Applications

Recommended Concentration
Sample
Western Blot
1 µg/mL
Recombinant Human Uteroglobin/SCGB1A1 (Catalog # 4218-UT)
Immunohistochemistry
8-25 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Data Examples

Immunohistochemistry Uteroglobin/SCGB1A1 in Human Lung. View Larger

Uteroglobin/SCGB1A1 in Human Lung. Uteroglobin/SCGB1A1 was detected in immersion fixed paraffin-embedded sections of human lung using 15 µg/mL Rat Anti-Human Uteroglobin/SCGB1A1 Monoclonal Antibody (Catalog # MAB4218) overnight at 4 °C. Before incubation with the primary antibody tissue was subjected to heat-induced epitope retrieval using Antigen Retrieval Reagent-Basic (Catalog # CTS013). Tissue was stained with the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). Specific labeling was localized to the cytoplasm of bronchiolar Clara cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Immunohistochemistry Uteroglobin/SCGB1A1 in Human Lung. View Larger

Uteroglobin/SCGB1A1 in Human Lung. Uteroglobin/SCGB1A1 was detected in immersion fixed paraffin-embedded sections of human lung using Rat Anti-Human Uteroglobin/SCGB1A1 Monoclonal Antibody (Catalog # MAB4218) at 15 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Rat HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS017) and counterstained with hematoxylin (blue). View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Preparation and Storage

Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
Reconstitution Buffer 1 (PBS)
Catalog #
Availability
Size / Price
Qty
RB01
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Uteroglobin/SCGB1A1

Uteroglobin, also called Clara cell secretory, phospholipid binding, 10 kDa or 16 kDa protein (CCSP, CCPBP, CC10 or CC16, respectively) is a small, non‑glycosylated secreted protein of the secretoglobin superfamily, (designated 1A, member 1) (1‑3). Its name is derived from its very high expression in the pre‑implantation uterus. It is produced by the non-ciliated, non-mucous secretory cells that predominate in lung bronchioles (Clara cells), and other non-ciliated epithelia that communicate with the external environment (1‑3). Expression is induced by steroid hormones such as estrogen, and enhanced by the non-steroid hormone prolactin (1). Uteroglobin is found in blood, urine and other body fluids (1). Human Uteroglobin cDNA encodes a 21 amino acid (aa) signal sequence and a 70 aa mature protein. It shares 53‑56% aa identity with mouse, rat, bovine, canine, equine or rabbit Uteroglobin, and is active in mice (4). The mature protein forms a disulfide-linked head-to-tail homodimer of 16 kDa (2, 5). This homodimer is thought to form a binding pocket that binds hydrophobic ligands such as phospholipids, progesterone and retinols (5). Sequestering of prostaglandins and leukotrienes is anti-inflammatory, while sequestering of carcinogens such as polychlorinated bisphenols is anti-tumorigenic (6‑8). Other immunoregulatory activities of Uteroglobin include cell migration inhibition (by binding the chemotaxis-related formyl peptide receptor FPR2 on dendritic cells), and the inhibition of T cell differentiation to a Th2 phenotype (9). A single nucleotide polymorphism of Uteroglobin, A38G, confers increased risk of asthma (10). Transglutaminase can crosslink Uteroglobin, either to itself or to other proteins such as the adhesion molecule fibronectin (3, 11). Binding of fibronectin to Uteroglobin in the kidney is thought to protect against nephropathy, while binding of the lipocalin-1 receptor has been reported to suppress cancer cell motility and invasion (12, 13).

References
  1. Mukherjee, A.B. et al. (2007) Endocr. Rev. 28:707.
  2. Singh, G. et al. (1988) Biochim. Biophys. Acta 950:329.
  3. Mantile, G. et al. (1993) J. Biol. Chem. 268:20343.
  4. Wang, S-Z. et al. (2003) J. Immunol. 171:1051.
  5. Umland, T.C. et al. (1994) Nat. Struct. Biol. 1:538.
  6. Mandal, A.K. et al. (2004) J. Exp. Med. 199:1317.
  7. Watson, T.M. et al. (2001) Am. J. Physiol. Lung Cell Mol. Physiol. 281:L1523.
  8. Yang, Y. et al. (2004) J. Biol. Chem. 279:29336.
  9. Ray, R. et al. (2006) FEBS Lett. 580:6022.
  10. Martin, A.C. et al. (2006) Am. J. Respir. Crit. Care Med. 173:617.
  11. Antico, G. et al. (2006) J. Cell Physiol. 207:553.
  12. Zheng, F. et al. (1999) Nat. Med. 5:1018.
  13. Zhang, Z. et al. (2006) Gene 369:66.
Entrez Gene IDs
7356 (Human); 22287 (Mouse); 25575 (Rat)
Alternate Names
Blastokinin; CC10; CC10Urine protein 1; CC16; CCPBP; CCSP; CCSPCCPBP; Clara cell phospholipid-binding protein; Clara cells 10 kDa secretory protein; Clara; PCB-BP; SCGB1A1; Secretoglobin family 1A member 1; secretoglobin, family 1A, member 1 (uteroglobin); UGB; UGBUP-1; UP1; Urinary protein 1; Uteroglobin

Product Datasheets

Citation for Human Uteroglobin/SCGB1A1 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

1 Citation: Showing 1 - 1

  1. Osteopontin Expression in Small Airway Epithelium in Copd is Dependent on Differentiation and Confined to Subsets of Cells
    Authors: MN Ali, M Mori, TCJ Mertens, P Siddhuraj, JS Erjefält, P Önnerfjord, PS Hiemstra, A Egesten
    Sci Rep, 2019;9(1):15566.
    Species: Human
    Sample Types: Whole Tissue
    Applications: IHC

FAQs

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Cell and Tissue Staining Kits

Immunohistochemistry Reagents

Isotype Controls

Rat IgG1 Isotype Control

Reconstitution Buffers

Secondary Antibodies

Reviews for Human Uteroglobin/SCGB1A1 Antibody

Average Rating: 4.8 (Based on 4 Reviews)

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Human Uteroglobin/SCGB1A1 Antibody
By Anonymous on 11/10/2019
Application: WB Sample Tested: Stimulated Human Bronchial Epithelial Cell Line (BEAS-2B) Species: Human

Cell lysis. 1:500 Dilution, incubate at 4 °C overnight.


Human Uteroglobin/SCGB1A1 Antibody
By Anonymous on 02/14/2019
Application: ICC/IF Sample Tested: bronchus Species: Human

DAPI - blue
Magenta -KRT5
Yellow - SCGB1A1


Human Uteroglobin/SCGB1A1 Antibody
By Anonymous on 12/20/2017
Application: ELISA Sample Tested: EDTA Plasma Species: Human

Human Uteroglobin/SCGB1A1 Antibody
By Anonymous on 11/27/2017
Application: ICC/IF Sample Tested: tracheal epithelial cells Species: Human

Cells were fixed in 4% Paraformaldehyde and stained with Human Uteroglobin/SCGB1A1 Antibody at 20µg/ml overnight at 4°C. As secondary antibodies Goat-anti-Rat-488 and Alexa Fluor647-Phalloidin were used.