Key Product Details

Species Reactivity

Human

Applications

Multiplex Immunofluorescence, Immunohistochemistry, COMET

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 1091512
View all VEGFR3/Flt-4 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line, NS0-derived human VEGFR3/Flt-4
Tyr25-Ile776
Accession # P35916

Specificity

Detects recombinant human VEGFR3 protein in Direct ELISA.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Human VEGFR3/Flt-4 Antibody

Detection of VEGFR3 in Human Tonsil via seqIF™ staining on COMET™​

VEGFR3 was detected in immersion fixed paraffin-embedded sections of human Tonsil using Rat Anti-Human VEGFR3, Monoclonal Antibody (Catalog #MAB11624) at 5ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and membrane. Protocol available in COMET™ Panel Builder.​

Detection of VEGFR3 in Human Hodgkin’s Lymphoma via seqIF™ staining on COMET™​

VEGFR3 was detected in immersion fixed paraffin-embedded sections of human Hodgkin's Lymphoma using Rat Anti-Human VEGFR3, Monoclonal Antibody (Catalog #MAB11624) at 5ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and membrane. Protocol available in COMET™ Panel Builder.​

Detection of VEGFR3 in Human Appendix via seqIF™ staining on COMET™​

VEGFR3 was detected in immersion fixed paraffin-embedded sections of human Appendix using Rat Anti-Human VEGFR3, Monoclonal Antibody (Catalog #MAB11624) at 5ug/mL at 37 ° Celsius for 8 minutes. Before incubation with the primary antibody, tissue underwent an all-in-one dewaxing and antigen retrieval preprocessing using PreTreatment Module (PT Module) and Dewax and HIER Buffer H (pH 9; Epredia Catalog # TA-999-DHBH). Tissue was stained using the Alexa Fluor™ 647 Goat anti-Rat IgG Secondary Antibody at 1:200 at 37 ° Celsius for 2 minutes. (Yellow; Lunaphore Catalog # DR647RT) and counterstained with DAPI (blue; Lunaphore Catalog # DR100). Specific staining was localized to the cytoplasm and membrane. Protocol available in COMET™ Panel Builder.​​

Detection of VEGFR3/Flt-4 in Human Liver.

VEGFR3/Flt-4 was detected in immersion fixed paraffin-embedded sections of human liver using Rat Anti-Human VEGFR3/Flt-4 Monoclonal Antibody (Catalog # MAB11624) at 5 µg/ml overnight at 4 °C. Before incubation with the primary antibody, tissue was subjected to heat-induced epitope retrieval using VisUCyte Antigen Retrieval Reagent-Basic (Catalog # VCTS021). Tissue was stained using the HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005) and counterstained with hematoxylin (blue). Specific staining was localized to the cell surface and cytoplasm of liver endothelial cells. View our protocol for Chromogenic IHC Staining of Paraffin-embedded Tissue Sections.

Applications for Human VEGFR3/Flt-4 Antibody

Application
Recommended Usage

COMET

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

3-25 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human liver

Multiplex Immunofluorescence

5 µg/mL
Sample: Immersion fixed paraffin-embedded sections of human Tonsil, Hodgkin's Lymphoma, and Appendix.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute lyophilized material at 0.2mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: VEGFR3/Flt-4

VEGFR2 (KDR/Flk-1), VEGFR1 (Flt-1) and VEGFR3 (Flt-4) belong to the class III subfamily of receptor tyrosine kinases (RTKs). All three receptors contain seven immunoglobulin-like repeats in their extracellular domains and kinase insert domains in their intracellular regions. The expression of VEGFR1, 2, and 3 is almost exclusively restricted to the endothelial cells. These receptors are likely to play essential roles in vasculogenesis and angiogenesis. VEGFR3 cDNA encodes a 1298 amino acid (aa) precursor with a 24 aa signal peptide. Mature VEGFR3 is composed of a 751 aa extracellular domain, a 22 aa transmembrane domain and a 482 aa cytoplasmic domain. Both VEGF-C and VEGF-D have been shown to bind and activate VEGFR3 (Flt-4). VEGFR3 is widely expressed in the early embryo but becomes restricted to lymphatic endothelia at later stages of development. It is likely that VEGFR3 may be important for lymph angiogenesis.

References

  1. Ferra, N. and R. Davis-Smyth (1997) Endocrine Reviews 18:4.

Long Name

Vascular Endothelial Growth Factor Receptor 3

Alternate Names

Flt-4, FLT4, VEGF R3

Entrez Gene IDs

2324 (Human); 14257 (Mouse)

Gene Symbol

FLT4

UniProt

P35916

Additional VEGFR3/Flt-4 Products

Product Documents for Human VEGFR3/Flt-4 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human VEGFR3/Flt-4 Antibody

For research use only

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Protocols

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