Human ZNF281 Antibody

  
  • Species Reactivity
    Human
  • Specificity
    Detects human ZNF281 in direct ELISAs and Western blots.
  • Source
    Monoclonal Mouse IgG1 Clone # 801706
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    E. coli-derived recombinant human ZNF281
    Asn681-Arg895
    Accession # Q9Y2X9
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
  • Simple Western
    50 µg/mL
    See below
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Human ZNF281 by Western Blot. Western blot shows lysates of BG01V human embryonic stem cells. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human ZNF281 Monoclonal Antibody (Catalog # MAB6350) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for ZNF281 at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Immunocytochemistry
ZNF281 in BG01V Human Embryonic Stem Cells. ZNF281 was detected in immersion fixed BG01V human embryonic stem cells using Mouse Anti-Human ZNF281 Monoclonal Antibody (Catalog # MAB6350) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red, upper panel; Catalog # NL007) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei. View our protocol for Fluorescent ICC Staining of Stem Cells on Coverslips.
Detection of Human ZNF281 by Simple WesternTM. Simple Western lane view shows lysates of BG01V human embryonic stem cells, loaded at 0.5 mg/mL. A specific band was detected for ZNF281 at approximately 107 kDa (as indicated) using 50 µg/mL of Mouse Anti-Human ZNF281 Monoclonal Antibody (Catalog # MAB6350). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Non-specific interaction with the 230 kDa Simple Western standard may be seen with this antibody.

Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: ZNF281
ZNF281 (Zinc Finger protein 28), also known as ZBP99 and GZP1, is a 100 kDa member of the Krueppel C2H2-type Zn-finger family of proteins. It is found in embryonic stem cells, placenta, and lymphocytes, and appears to be necessary for the maintenance of pluripotency in stem cells. ZNF281 both represses (STAT3) and activates (Nanog) multiple genes, and is known to form a complex with Nanog, Oct4 and Sox2. Human ZFN281 is 895 amino acids in length. It contains one Gly-rich region (aa 4-37), a poly-Pro motif (aa 90-96) and four consecutive C2H2-type zinc finger domains (aa 261-367). There are phosphorylation sites at Ser255, 395, 484, 785 and 807. Potential alternate start sites exist at Met296 and Met434. Over aa 681-895, human ZFN281 shares 99% aa identity with mouse ZFN281.
  • Long Name:
    Zinc Finger Protein 281
  • Entrez Gene IDs:
    23528 (Human); 226442 (Mouse); 305083 (Rat)
  • Alternate Names:
    FLJ20557; GZP1; ZBP-99; zinc finger protein 416; ZNF281; ZNP-99
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