IkB-alpha [p Ser36] Antibody (8G1X10)

Name: IkB-alpha [p Ser36] Antibody (8G1X10)
Brand: Novus Biologicals
SKU: NBP3-16912
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP3-16912

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 8G1X10 expressed in HEK293

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Product Specifications

Immunogen

A synthetic phosphorylated peptide around S36 of human IkB-alpha (P25963). LDSMK

Modification

p Ser 36

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IkB-alpha [p Ser36] Antibody (8G1X10)

Western Blot: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Western blot analysis of extracts of various cell lines, using IkB-alpha Rabbit mAb (NBP3-16912) at 1:1000 dilution.THP-1 cells were treated by PMA/TPA (80 nM) at 37C for overnight or treated by LPS (1 ug/mL) at 37C for 6 hours.Both NIH/3T3 cells and C6 cells were treated by TNF-alpha (20 ng/mL) at 37C for 30 minutes. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA. Detection: ECL Enhanced Kit. Exposure time: 3min.

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] -

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry analysis of paraffin-embedded Human thyroid cancer using IkB-alpha Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] -

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry analysis of paraffin-embedded Human colon carcinoma using IkB-alpha Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] -

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry analysis of paraffin-embedded Human colon using IkB-alpha Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] -

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using IkB-alpha Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] -

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry analysis of paraffin-embedded Mouse intestin using IkB-alpha Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] -

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry analysis of paraffin-embedded Mouse liver using IkB-alpha Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] -

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using IkB-alpha Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] -

Immunohistochemistry: IkB-alpha [p Ser36] Antibody (8G1X10) [NBP3-16912] - Immunohistochemistry analysis of paraffin-embedded Human spleen using IkB-alpha Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

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Applications for IkB-alpha [p Ser36] Antibody (8G1X10)

Application

Recommended Usage

Immunohistochemistry

1:50 - 1:200

Western Blot

1:500 - 1:1000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: IkB-alpha

NF-kB is silenced in the cytoplasm by an inhibitory protein, IkB (1). Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-6). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Six members of IkB family members have been identified (1). One of the first gene induced following NF-kB activation is IkBa.

Long Name

I-kappa-B-alpha

Alternate Names

IkBalpha, MAD-3, NFKBIA

Gene Symbol

NFKBIA

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Product Specific Notices for IkB-alpha [p Ser36] Antibody (8G1X10)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.