IKK beta Antibody (SN63-02)

Name: IKK beta Antibody (SN63-02)
Brand: Novus Biologicals
SKU: NBP2-67659
Price: 439 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-67659

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Product Documents

Recombinant Monoclonal Antibody

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # SN63-02 expressed in HEK293

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Product Specifications

Immunogen

Recombinant protein within Human IKK beta aa 450-652 / 756. (SwissProt: O14920 Human; SwissProt: O15111 Human; SwissProt: O88351 Mouse; SwissProt: Q60680 Mouse; SwissProt: Q9QY78 Rat)

Localization

Cytoplasm, Nucleus, Membrane raft.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for IKK beta Antibody (SN63-02)

Western Blot: IKK beta Antibody (SN63-02) [NBP2-67659]

Western Blot: IKK beta Antibody (SN63-02) [NBP2-67659] - Western blot analysis of IKK beta on different lysates. Proteins were transferred to a PVDF membrane and blocked with 5% BSA in PBS for 1 hour at room temperature. The primary antibody (1/500) was used in 5% BSA at room temperature for 2 hours. Goat Anti-

Immunocytochemistry/ Immunofluorescence: IKK beta Antibody (SN63-02) [NBP2-67659]

Immunocytochemistry/Immunofluorescence: IKK beta Antibody (SN63-02) [NBP2-67659] - Staining IKK alpha+IKK beta in B-6F1 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Western Blot: IKK beta Antibody (SN63-02) [NBP2-67659]

Western Blot: IKK beta Antibody (SN63-02) [NBP2-67659] - Analysis of IKK alpha+IKK beta on different lysates using anti-IKK alpha+IKK beta antibody at 1/1,000 dilution. Positive control: Lane 1: Hela Lane 2: Daudi Lane 3: A431

Immunocytochemistry/ Immunofluorescence: IKK beta Antibody (SN63-02) [NBP2-67659]

Immunocytochemistry/Immunofluorescence: IKK beta Antibody (SN63-02) [NBP2-67659] - Staining IKK alpha+IKK beta in Hela cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

Immunocytochemistry/ Immunofluorescence: IKK beta Antibody (SN63-02) [NBP2-67659]

Immunocytochemistry/Immunofluorescence: IKK beta Antibody (SN63-02) [NBP2-67659] - Staining IKK alpha+IKK beta in C2C12 cells (green). The nuclear counter stain is DAPI (blue). Cells were fixed in paraformaldehyde, permeabilised with 0.25% Triton X100/PBS.

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Applications for IKK beta Antibody (SN63-02)

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:500

Western Blot

1:500-1:1000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

TBS (pH7.4), 0.05% BSA, 40% Glycerol

Preservative

0.05% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: IKK beta

NF-kB (nuclear factor kB) is sequestered in the cytoplasm by IkB family of inhibitory proteins that mask the nuclear localization signal of NF-kB thereby preventing translocation of NF-kB to the nucleus (1). External stimuli such as tumor necrosis factor or other cytokines results in phosphorylation and degradation of IkB releasing NF-kB dimers. NF-kB dimer subsequently translocates to the nucleus and activates target genes. Synthesis of IkBa is autoregulated (2). IkB proteins are phosphorylated by IkB kinase complex consisting of at least three proteins, IKK1/a, IKK2/b, and IKK3/g (3-5). IKK1/a and IKK2/b are phosphorylated by NF-kB-inducing kinase (NIK) (6) and MAP kinase kinase kinase-1 (MEKK1) (7), respectively. Recent studies have shown that IKK2/b, not IKK1/a, is the target proinflammatory stimuli (9). Targeted disruption of IKK2/b gene in mice also results in extensive liver damage from apotopsis and death as embryo (9).

Long Name

IkB Kinase beta

Alternate Names

IkBKB, IKK2, NFKBIKB

Gene Symbol

IKBKB

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Product Documents for IKK beta Antibody (SN63-02)

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Product Specific Notices for IKK beta Antibody (SN63-02)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.