IKK epsilon/IKBKE Antibody [FITC]

Novus Biologicals | Catalog # NBP1-76800F

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

FITC (Excitation = 495 nm, Emission = 519 nm)

Antibody Source

Polyclonal Rabbit IgG
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Product Specifications

Immunogen

Antibody was raised against a peptide corresponding to 16 amino acids near the carboxy terminus of human IKK epsilon/IKBKE. The immunogen is located within the last 50 amino acids of IKK epsilon/IKBKE.

Reactivity Notes

0

Specificity

It has no cross response to IKKa, IKKb, or IKKy. Human IKK epsilon/IKBKE has 2 isoforms, including isoform 1 (716aa, 80kD) and isoform 2 (631aa, 70kD). Mouse IKK epsilon/IKBKE has one isoform (717aa, 81kD) and Rat IKK epsilon/IKBKE also has one isoform (717aa, 80kD). NBP1-76800 can detect human, mouse and possibly rat.

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Applications for IKK epsilon/IKBKE Antibody [FITC]

Application
Recommended Usage

ELISA

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry/ Immunofluorescence

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry

Optimal dilutions of this antibody should be experimentally determined.

Immunohistochemistry-Paraffin

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Peptide affinity purified

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IKK epsilon

Nuclear Factor kappa B (NF-kB) is a ubiquitous transcription factor and an essential mediator of gene expression during the activation of immune and inflammatory responses. NF-kB mediates the expression of a great variety of genes in response to extracellular stimuli. NF-kB is associated with IkB proteins in the cytoplasm of the cell, which inhibit NF-kB activity. IkB proteins are phosphorylated by an IkB kinase complex consisting of at least three proteins, IKKa, IKKb, and IKKg. Isolated from a cDNA library of LPS-stimulated mouse macrophage cells, a novel molecule in the IKK complex has been recently identified and designated IKKi and/or IKKe. IKKe is required for the activation of NF-kB by mitogens and T cell receptors but not by TNFa or IL-1. LPS increases the IKKe mRNA level in mouse macrophage cell lines. This protein has significant sequence homology with kinase domains of IKKa and IKKb. Overexpression of wild type IKKe in cells phosphorylates Ser32 and Ser36 of IkBa.

Long Name

IkB Kinase epsilon

Alternate Names

IKBKE, IKK-I

Gene Symbol

IKBKE

Additional IKK epsilon Products

Product Documents for IKK epsilon/IKBKE Antibody [FITC]

Certificate of Analysis

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Product Specific Notices for IKK epsilon/IKBKE Antibody [FITC]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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