Key Product Details

Species Reactivity

Human

Applications

Western Blot, Intracellular Staining by Flow Cytometry, Immunocytochemistry, CyTOF-ready

Label

Biotin

Antibody Source

Monoclonal Mouse IgG1 Clone # 9906
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Product Specifications

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human IL‑5
Ile20-Ser134
Accession # P05113

Specificity

Detects human IL-5 in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant mouse IL‑5 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Applications for IL-5 Antibody (9906) [Biotin]

Application
Recommended Usage

CyTOF-ready

Optimal dilutions of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilutions of this antibody should be experimentally determined.

Intracellular Staining by Flow Cytometry

Optimal dilutions of this antibody should be experimentally determined.

Western Blot

Optimal dilutions of this antibody should be experimentally determined.
Application Notes
Optimal dilution of this antibody should be experimentally determined.

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Formulation

PBS

Preservative

0.05% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: IL-5

IL-5 (T cell replacing factor (TRF), eosinophil differentiation factor (EDF)) is a hematopoietic growth factor that stimulates the proliferation, differentiation and activation of eosinophils. It is produced by mast cells, T cells and eosinophils and has an important role in activating cell-mediated immunity following parasitic infections. It belongs to the same cytokine family as IL-3 and GM-CSF. The biologically active IL-5 consists of an antiparallel homodimer linked by disulfide bonds. Human and murine IL-5 are 70% identical at the amino acid level and show species cross-reactivity. IL-5 exerts its effects on target cells by binding to specific cell surface receptors.

Long Name

Interleukin 5

Alternate Names

BCDF mu, BCGFII, EDF, Eo-CSF, IL5, TRF

Gene Symbol

IL5

Additional IL-5 Products

Product Documents for IL-5 Antibody (9906) [Biotin]

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for IL-5 Antibody (9906) [Biotin]

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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