Insulin Antibody (4C3Y9)

Novus Biologicals | Catalog # NBP3-15643

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 4C3Y9 expressed in HEK293
View all Insulin Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Insulin (P01308). MALWMRLLPLLALLALWGPDPAAAFVNQHLCGSHLVEALYLVCGERGFFYTPKTRREAEDLQVGQVELGGGPGAGSLQPLALEGSLQKRGIVEQCCTSIC

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

12 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Insulin Antibody (4C3Y9)

Western Blot: Insulin Antibody (4C3Y9) [NBP3-15643]

Western Blot: Insulin Antibody (4C3Y9) [NBP3-15643]

Western Blot: Insulin Antibody (4C3Y9) [NBP3-15643] - Western blot analysis of extracts of various cell lines, using Insulin antibody (NBP3-15643) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunohistochemistry-Paraffin: Insulin Antibody (4C3Y9) [NBP3-15643]

Immunohistochemistry-Paraffin: Insulin Antibody (4C3Y9) [NBP3-15643]

Immunohistochemistry-Paraffin: Insulin Antibody (4C3Y9) [NBP3-15643] - Immunohistochemistry of paraffin-embedded rat pancreatic islet using Insulin Rabbit mAb (NBP3-15643) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: Insulin Antibody (4C3Y9) [NBP3-15643]

Immunohistochemistry-Paraffin: Insulin Antibody (4C3Y9) [NBP3-15643]

Immunohistochemistry-Paraffin: Insulin Antibody (4C3Y9) [NBP3-15643] - Immunohistochemistry of paraffin-embedded mouse pancreatic islet using Insulin Rabbit mAb (NBP3-15643) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Insulin Antibody (4C3Y9)

Immunohistochemistry: Insulin Antibody (4C3Y9) [NBP3-15643] -

Immunohistochemistry: Insulin Antibody (4C3Y9) [NBP3-15643] -Confocal imaging of human pancreas using Insulin Rabbit mAb (at dilution of 1:100) (Red). DAPI was used for nuclear staining (blue). Objective: 40x. Perform high pressure antigen retrieval with 10 mM citrate buffer pH 6.0 before commencing with IF staining protocol.

Applications for Insulin Antibody (4C3Y9)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:200 - 1:2000

Immunohistochemistry

1:500 - 1:500

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Insulin

After removal of the precursor signal peptide, proinsulin is post-translationally cleaved into two chains (peptide A and peptide B) that are covalently linked via two disulfide bonds. Binding of this mature form of insulin to the insulin receptor (INSR) stimulates glucose uptake. A variety of mutant alleles with changes in the coding region have been identified. There is a read-through gene, INS-IGF2, which overlaps with this gene at the 5' region and with the IGF2 gene at the 3' region. (provided by RefSeq)

Alternate Names

IDDM2, ILPR, INS, IRDN, MODY10

Gene Symbol

INS

Additional Insulin Products

Product Documents for Insulin Antibody (4C3Y9)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for Insulin Antibody (4C3Y9)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Insulin Antibody (4C3Y9)

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  • Q: Will you please recommend me antibodies to insulin, the most suitable for immunohistochemistry in the pancreas of guinea pigs? Desirable to be conjugated with some pigment and there was no need for secondary antibodies.

    A:

    I have carried out a search and the best antibody I recommend is NB600-1488. This is a primary unconjugated antibody however and you will need a secondary. If you click on the product link and then click on related products, a list of all recommended secondary antibodies will appear.

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