Q: I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.

A: I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Integrin beta 1/CD29 Antibody (12G10) - BSA Free

Novus Biologicals | Catalog # NB100-63255

(1)

Citations (4)

Product Documents

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Frozen, ELISA, Flow Cytometry, Knockdown Validated

Cited:

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 12G10

Format

BSA Free

View all Integrin beta 1/CD29 Primary Antibodies »

Product Specifications

Immunogen

Purified human beta1 integrin preparation from HT1080 fibrosarcoma cell extract

Epitope

Epitope appears to be located around residues 207-218 in the b1 subunit putative A-domain.

Reactivity Notes

Predicted cross-reactivities: Mink, Rabbit

Localization

Type 1 membrane protein

Marker

Stem Cell Marker

Specificity

NB100-63255 recognises the human beta1 integrin. This has been shown to bind to a ligand induced binding site, binding being increased in the presence of fibronectin ligand. This also enhances alpha 5 - beta 1 - fibronectin interactions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Integrin beta 1/CD29 Antibody (12G10) - BSA Free

Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]

Integrin-beta-1-CD29-Antibody-12G10-Immunocytochemistry-Immunofluorescence-NB100-63255-img0005.jpg

Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]

Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) [Alexa Fluor 488] [NB100-63255AF488] - A cell surface stain was performed on A549 cells with Integrin beta 1/CD29 (12G10) antibody NB100-63255AF488 (blue) and a matched isotype control NBP1-97005AF488 (orange). Cells were incubated in an antibody dilution of 5 ug/mL for 20 minutes at room temperature. Both antibodies were conjugated to Alexa Fluor 488. Image using the Alexa Fluor 488 form of this antibody.

Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]

Immunocytochemistry/Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Human salivary gland progenitor cells express integrin beta 1 by day 1 in three-dimensional hyaluronan-based hydrogels. Image from verified customer review.

Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255]

Flow Cytometry: Integrin beta 1/CD29 Antibody (12G10) [NB100-63255] - Staining of human peripheral blood monocytes with MOUSE ANTI HUMAN CD29.

Immunocytochemistry/ Immunofluorescence: Integrin beta 1/CD29 Antibody (12G10) - BSA Free [NB100-63255] -

Quantification of knockdown of beta 1 integrin in hS/PC microstructures. hS/PC microstructures formed in HA-based hydrogels and were transfected with scrambled siRNA or ITGB1 siRNA. Immunocytochemistry was used to determine beta 1 integrin expression and spatial distribution of beta 1 integrin 46 h post-transfection. Image analysis software was used to quantify beta 1 integrin expression at microstructure surfaces from confocal micrographs and coverage indices were calculated for scrambled siRNA (A) and ITGB1 siRNA (B) groups where a 63% reduction in beta 1 integrin expression at microstructure surfaces was quantified (C). Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31750298), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

View 2 more images

Applications for Integrin beta 1/CD29 Antibody (12G10) - BSA Free

Application

Recommended Usage

ELISA

10 ug/mL

Flow Cytometry

1:25-1:50

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Reviewed Applications

Read 1 review rated 5 using NB100-63255 in the following applications:

Immunocytochemistry (1 Review)

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Formulation, Preparation, and Storage

Purification

Protein G purified

Formulation

PBS

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

1.0 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: Integrin beta 1/CD29

Integrin beta 1, also known as CD29, is a 130 kDa transmembrane glycoprotein that forms noncovalent complexes with various Integrin alpha subunits (including alpha 1, alpha 2, alpha 3, alpha 4, alpha 5, and alpha 6, also known as CD49a, CD49b, CD49c, CD49d, CD49e, and CD49f, respectively) to form the functional receptors that bind to specific extracellular matrix proteins. Integrin receptors are involved in the regulation of a variety of important biological functions, including embryonic development, wound repair, hemostasis, and prevention of programmed cell death. They are also implicated in abnormal pathological states such as tumor directed angiogenesis, tumor cell growth, and metastasis. These heterodimeric receptors bridge the cytoplasmic actin cytoskeleton with proteins present in the extracellular matrix and/or on adjacent cells. The clustering of integrins on a cell surface leads to the formation of focal contacts. Interactions between integrins and the extracellular matrix lead to activation of signal transduction pathways and regulation of gene expression.

Alternate Names

CD29, ITGB1

Entrez Gene IDs

3688 (Human)

Gene Symbol

ITGB1

UniProt

P05556

Additional Integrin beta 1/CD29 Products

Product Documents for Integrin beta 1/CD29 Antibody (12G10) - BSA Free

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Certificate of Analysis

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Product Specific Notices for Integrin beta 1/CD29 Antibody (12G10) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adhesion Molecules

Adhesion Molecules on Endothelial Cells

Adipose-derived Stem Cell Markers

Cancer Biomarkers

Cardiac Stem Cell Markers

Epithelial Cell Polarity

Integrins and Associated Molecules

Mesenchymal Stem Cell Markers

Skin Stem Cell Markers

Citations for Integrin beta 1/CD29 Antibody (12G10) - BSA Free

Customer Reviews for Integrin beta 1/CD29 Antibody (12G10) - BSA Free (1)

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Integrin beta 1/CD29 Antibody (12G10)

Name: Anonymous

Application: Immunocytochemistry

Sample Tested: Human salivary gland primary progenitor cells

Species: Human

Verified Customer | Posted 11/29/2018

Human salivary gland progenitor cells express integrin beta 1 by day 1 in three-dimensional hyaluronan-based hydrogels.

Used dilution of 1:100

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Protocols

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FAQs for Integrin beta 1/CD29 Antibody (12G10) - BSA Free

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Q: I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.

A: I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.