Q: I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.

A: I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.

Integrin beta 1/CD29 Antibody (CL15606) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-44157

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Human

Applications

Immunohistochemistry-Paraffin, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # CL15606

Format

Azide and BSA Free

View all Integrin beta 1/CD29 Primary Antibodies »

Product Specifications

Immunogen

This antibody was generated using a recombinant protein sequence of P05556, with the exact immunogen sequence remaining proprietary.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Description

Novus Biologicals Mouse Integrin beta 1/CD29 Antibody (CL15606) - Azide and BSA Free (NBP3-43675) is a monoclonal antibody validated for use in IHC and WB. All Novus Biologicals antibodies are covered by our 100% guarantee.

Scientific Data Images for Integrin beta 1/CD29 Antibody (CL15606) - Azide and BSA Free

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP3-44157]

Analysis in human placenta and cerebral cortex tissues using NBP3-44157 antibody. Corresponding Integrin beta 1/CD29 RNA-seq data are presented for the same tissues.

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP3-44157]

Staining of human cerebral cortex shows strong membranous positivity in endothelial cells.

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP3-44157]

Staining of human placenta shows strong membranous positivity in endothelial cells.

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP3-44157]

Staining of human liver shows strong positivity in sinusoids.

Western Blot: Integrin beta 1/CD29 Antibody [NBP3-44157]

Analysis in human cell lines A-549 and HEK293 using Anti-Integrin beta 1/CD29 antibody. Corresponding Integrin beta 1/CD29 RNA-seq data are presented for the same cell lines. Loading control: Anti-PARP1.

Immunohistochemistry-Paraffin: Integrin beta 1/CD29 Antibody [NBP3-44157]

Staining of human testis shows strong membranous positivity in peritubular myoid cells.

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Applications for Integrin beta 1/CD29 Antibody (CL15606) - Azide and BSA Free

Application

Recommended Usage

Immunohistochemistry-Paraffin

1:2500-1:5000

Western Blot

1 ug/ml

Application Notes

For IHC-Paraffin, HIER pH 6 retrieval is recommended.

Formulation, Preparation, and Storage

Purification

Protein A purified

Reconstitution

Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.

Formulation

Lyophilized from a 0.2 um filtered solution in PBS with Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Integrin beta 1/CD29

Integrin beta-1 (ITGB1, CD29, VLA-beta) is the beta subunit found in the integrin families, forming a heterodimer integrin receptor through non-covalent bonding with various integrin alpha subunits. Integrin heterodimer containing Integrin beta-1 binds to various cell surface and extracellular proteins (CD49a-f, CD51) to mediate cell to cell and cell to matrix adhesion (1). Integrin beta-1 plays a critical role in the cell adhesion and recognition in embryogenesis, hemostasis, immune response, tissue repair, metastatic diffusion of tumor cells and development (2, 3, 4).

Alternate Names

CD29, ITGB1

Gene Symbol

ITGB1

Additional Integrin beta 1/CD29 Products

Product Documents for Integrin beta 1/CD29 Antibody (CL15606) - Azide and BSA Free

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Download SDS

Product Specific Notices for Integrin beta 1/CD29 Antibody (CL15606) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Adhesion Molecules

Adhesion Molecules on Endothelial Cells

Adipose-derived Stem Cell Markers

Cancer Biomarkers

Cardiac Stem Cell Markers

Epithelial Cell Polarity

Integrins and Associated Molecules

Mesenchymal Stem Cell Markers

Skin Stem Cell Markers

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs for Integrin beta 1/CD29 Antibody (CL15606) - Azide and BSA Free

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Q: I'm looking for a good antibody for integrins that works in rats. I would need it for Western Blot, Immunocyto- as well as Immunohistochemistry. I'm especially interested in beta1.

A: I would recommend NB110-57123 as it has been validated using rat samples in WB, IHC-P, and FACS. Although we do not list ICC on the datasheet for this antibody I believe it will work in ICC because it works in Flow Cytometry and has been validated to stain tissue. We have several other Integrin targets. To search for what you are looking for I recommend typing Integrin into the search area at the top of our website, then review the list of suggested targets that poplulate below the search. Once you click on a particular Integrin you can narrow the results with our filter. I recommend filtering to primary antibodies, by target name, species of interest you can filter to rat. If you so choose you can also filter further by application, host, clonality, etc.