JNK1 Antibody (2F11) - Azide and BSA Free

Name: JNK1 Antibody (2F11) - Azide and BSA Free
Brand: Novus Biologicals
SKU: H00005599-M11
Price: 479 USD
Availability: InStock

Novus Biologicals | Catalog # H00005599-M11

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Product Documents

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human

Applications

Western Blot, ELISA, Sandwich ELISA, Immunocytochemistry/ Immunofluorescence, Proximity Ligation Assay, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2a Kappa Clone # 2F11

Format

Azide and BSA Free

View all JNK1 Primary Antibodies »

Product Specifications

Immunogen

MAPK8 (NP_002741.1, 1 a.a. ~ 384 a.a) full-length recombinant protein with GST tag. MW of the GST tag alone is 26 KDa. MSRSKRDNNFYSVEIGDSTFTVLKRYQNLKPIGSGAQGIVCAAYDAILERNVAIKKLSRPFQNQTHAKRAYRELVLMKCVNHKNIIGLLNVFTPQKSLEEFQDVYIVMELMDANLCQVIQMELDHERMSYLLYQMLCGIKHLHSAGIIHRDLKPSNIVVKSDCTLKILDFGLARTAGTSFMMTPYVVTRYYRAPEVILGMGYKENVDLWSVGCIMGEMVCHKILFPGRDYIDQWNKVIEQLGTPCPEFMKKLQPTVRTYVENRPKYAGYSFEKLFPDVLFPADSEHNKLKASQARDLLSKMLVIDASKRISVDEALQHPYINVWYDPSEAEAPPPKIPDKQLDEREHTIEEWKELIYKEVMDLEERTKNGVIRGQPSPLAQVQQ

Specificity

MAPK8 - mitogen-activated protein kinase 8 (2F11)

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2a Kappa

Description

Quality control test: Antibody Reactive Against Recombinant Protein.

Scientific Data Images for JNK1 Antibody (2F11) - Azide and BSA Free

Western Blot: JNK1 Antibody (2F11) [H00005599-M11]

Western Blot: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of MAPK8 expression in transfected 293T cell line by MAPK8 monoclonal antibody (M11), clone 2F11. Lane 1: MAPK8 transfected lysatE (48.3 KDa). Lane 2: Non-transfected lysate.

Immunocytochemistry/ Immunofluorescence: JNK1 Antibody (2F11) [H00005599-M11]

Immunocytochemistry/Immunofluorescence: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of monoclonal antibody to MAPK8 on HeLa cell. Antibody concentration 10 ug/ml

Western Blot: JNK1 Antibody (2F11) [H00005599-M11]

Western Blot: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of MAPK8 over-expressed 293 cell line, cotransfected with MAPK8 Validated Chimera RNAi or non-transfected control. Blot probed with H00005599-M11. GAPDH (36.1 kDa) used as loading control.

Proximity Ligation Assay: JNK1 Antibody (2F11) [H00005599-M11]

Proximity Ligation Assay: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of protein-protein interactions between CRK and MAPK8. HeLa cells were stained with anti-CRK rabbit purified polyclonal 1:1200 and anti-MAPK8 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction com

Proximity Ligation Assay: JNK1 Antibody (2F11) [H00005599-M11]

Proximity Ligation Assay: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of protein-protein interactions between PXN and MAPK8. Huh7 cells were stained with anti-PXN rabbit purified polyclonal 1:1200 and anti-MAPK8 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction com

Proximity Ligation Assay: JNK1 Antibody (2F11) [H00005599-M11]

Proximity Ligation Assay: JNK1 Antibody (2F11) [H00005599-M11] - Analysis of protein-protein interactions between BAD and MAPK8. Mahlavu cells were stained with anti-BAD rabbit purified polyclonal 1:1200 and anti-MAPK8 mouse monoclonal antibody 1:50. Each red dot represents the detection of protein-protein interaction

Sandwich ELISA: JNK1 Antibody (2F11) [H00005599-M11] - Detection limit for recombinant GST tagged MAPK8 is 1 ng/ml as a capture antibody.

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Applications for JNK1 Antibody (2F11) - Azide and BSA Free

Application

Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:10-1:2000

Western Blot

1:500

Application Notes

Antibody reactivity against recombinant protein for WB. It has been used for RNAi Validation and ELISA.

Formulation, Preparation, and Storage

Purification

IgG purified

Formulation

In 1x PBS, pH 7.4

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: JNK1

The protein encoded by this gene is a member of the MAP kinase family. MAP kinases act as an integration point for multiple biochemical signals, and are involved in a wide variety of cellular processes such as proliferation, differentiation, transcription regulation and development. This kinase is activated by various cell stimuli, and targets specific transcription factors, and thus mediates immediate-early gene expression in response to cell stimuli. The activation of this kinase by tumor-necrosis factor alpha (TNF-alpha) is found to be required for TNF-alpha induced apoptosis. This kinase is also involved in UV radiation induced apoptosis, which is thought to be related to cytochrom c-mediated cell death pathway. Studies of the mouse counterpart of this gene suggested that this kinase play a key role in T cell proliferation, apoptosis and differentiation. Four alternatively spliced transcript variants encoding distinct isoforms have been reported. [provided by RefSeq]

Long Name

C-Jun N-terminal Kinase 1

Alternate Names

MAPK8, PRKM8, SAPK1

Entrez Gene IDs

5599 (Human)

Gene Symbol

MAPK8

UniProt

NP_002741

Additional JNK1 Products

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Product Specific Notices for JNK1 Antibody (2F11) - Azide and BSA Free

This product is produced by and distributed for Abnova, a company based in Taiwan.

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.