KLF4 Antibody (7B7U1)

Novus Biologicals | Catalog # NBP3-15469

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 7B7U1 expressed in HEK293
View all KLF4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 300-400 of human KLF4 (O43474). PAAHDFPLGRQLPSRTTPTLGLEEVLSSRDCHPALPLPPGFHPHPGPNYPSFLPDQMQPQVPPLHYQGQSRGFVARAGEPCVCWPHFGTHGMMLTPPSSPL

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

55 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KLF4 Antibody (7B7U1)

Western Blot: KLF4 Antibody (7B7U1) [NBP3-15469]

Western Blot: KLF4 Antibody (7B7U1) [NBP3-15469]

Western Blot: KLF4 Antibody (7B7U1) [NBP3-15469] - Analysis of extracts of Mouse lung, using KLF4 Rabbit mAb (NBP3-15469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 3min.
Immunocytochemistry/ Immunofluorescence: KLF4 Antibody (7B7U1) [NBP3-15469]

Immunocytochemistry/ Immunofluorescence: KLF4 Antibody (7B7U1) [NBP3-15469]

Immunocytochemistry/Immunofluorescence: KLF4 Antibody (7B7U1) [NBP3-15469] - Immunofluorescence analysis of U-2 OS cells using KLF4 Rabbit mAb (NBP3-15469) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: KLF4 Antibody (7B7U1) [NBP3-15469]

Immunohistochemistry-Paraffin: KLF4 Antibody (7B7U1) [NBP3-15469]

Immunohistochemistry-Paraffin: KLF4 Antibody (7B7U1) [NBP3-15469] - Mouse spinal cord using KLF4 Rabbit mAb (NBP3-15469) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Western Blot: KLF4 Antibody (7B7U1) [NBP3-15469]

Western Blot: KLF4 Antibody (7B7U1) [NBP3-15469]

Western Blot: KLF4 Antibody (7B7U1) [NBP3-15469] - Analysis of extracts of various cell lines, using KLF4 Rabbit mAb (NBP3-15469) at 1:1000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 30s.
Immunocytochemistry/ Immunofluorescence: KLF4 Antibody (7B7U1) [NBP3-15469]

Immunocytochemistry/ Immunofluorescence: KLF4 Antibody (7B7U1) [NBP3-15469]

Immunocytochemistry/Immunofluorescence: KLF4 Antibody (7B7U1) [NBP3-15469] - Immunofluorescence analysis of C6 cells using KLF4 Rabbit mAb (NBP3-15469) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Immunohistochemistry-Paraffin: KLF4 Antibody (7B7U1) [NBP3-15469]

Immunohistochemistry-Paraffin: KLF4 Antibody (7B7U1) [NBP3-15469]

Immunohistochemistry-Paraffin: KLF4 Antibody (7B7U1) [NBP3-15469] - Rat brain using KLF4 Rabbit mAb (NBP3-15469) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunohistochemistry-Paraffin: KLF4 Antibody (7B7U1) [NBP3-15469]

Immunohistochemistry-Paraffin: KLF4 Antibody (7B7U1) [NBP3-15469]

Immunohistochemistry-Paraffin: KLF4 Antibody (7B7U1) [NBP3-15469] - Human esophageal using KLF4 Rabbit mAb (NBP3-15469) at dilution of 1:100 (40x lens).Perform microwave antigen retrieval with 10 mM PBS buffer pH 7.2 before commencing with IHC staining protocol.
Immunoprecipitation: KLF4 Antibody (7B7U1) [NBP3-15469]

Immunoprecipitation: KLF4 Antibody (7B7U1) [NBP3-15469]

Immunoprecipitation: KLF4 Antibody (7B7U1) [NBP3-15469] - Analysis of 300ug extracts of HeLa cells using 3ug KLF4 antibody (NBP3-15469). Western blot was performed from the immunoprecipitate using KLF4 antibody (NBP3-15469) at a dilition of 1:1000.
KLF4 Antibody (7B7U1)

Immunohistochemistry: KLF4 Antibody (7B7U1) [KLF4] -

Immunohistochemistry: KLF4 Antibody (7B7U1) [KLF4] - Immunohistochemistry analysis of paraffin-embedded Mouse brain using KLF4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
KLF4 Antibody (7B7U1)

Immunocytochemistry/ Immunofluorescence: KLF4 Antibody (7B7U1) [KLF4] -

Immunocytochemistry/ Immunofluorescence: KLF4 Antibody (7B7U1) [KLF4] - Confocal imaging of U-2 OS cells using KLF4 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.
KLF4 Antibody (7B7U1)

Immunohistochemistry: KLF4 Antibody (7B7U1) [KLF4] -

Immunohistochemistry: KLF4 Antibody (7B7U1) [KLF4] - Immunohistochemistry analysis of paraffin-embedded Mouse kidney using KLF4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
KLF4 Antibody (7B7U1)

Immunohistochemistry: KLF4 Antibody (7B7U1) [KLF4] -

Immunohistochemistry: KLF4 Antibody (7B7U1) [KLF4] - Immunohistochemistry analysis of paraffin-embedded Human liver cancer using KLF4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.
KLF4 Antibody (7B7U1)

Immunohistochemistry: KLF4 Antibody (7B7U1) [KLF4] -

Immunohistochemistry: KLF4 Antibody (7B7U1) [KLF4] - Immunohistochemistry analysis of paraffin-embedded Human colon using KLF4 Rabbit mAb at dilution of 1:200 (40x lens). High pressure antigen retrieval performed with 0.01M Citrate Bufferr (pH 6.0) prior to IHC staining.

Applications for KLF4 Antibody (7B7U1)

Application
Recommended Usage

ELISA

Recommended starting concentration is 1 μg/mL. Please optimize the concentration based on your specific assay requirements.

Immunocytochemistry/ Immunofluorescence

1:100 - 1:1000

Immunohistochemistry

1:200 - 1:2000

Immunohistochemistry-Paraffin

1:200 - 1:2000

Immunoprecipitation

0.5μg-4μg antibody for 200μg-400μg extracts of whole cells

Western Blot

1:1000 - 1:2000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: KLF4

KLF4 is a transcription factor that works with Sp1 to activate the Laminin gamma1 chain gene. It binds the CACCC core sequence. KLF4 may be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 also has roles as both an oncogene and a tumor suppressor.

Long Name

Kruppel-Like Factor 4

Alternate Names

EZF

Gene Symbol

KLF4

Additional KLF4 Products

Product Documents for KLF4 Antibody (7B7U1)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for KLF4 Antibody (7B7U1)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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