KLF4 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-83940

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation, Biological Validation

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Cited:

Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, Chemotaxis, IF/IHC, Knockdown Validated

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all KLF4 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: ETEEFNDLLDLDFILSNSLTHPPESVAATVSSSASASSSSSPSSSGPASAPSTCSFTYPIRAGNDPGVAPGGTGGGLLYGRESAPPPTAPFNLADINDVSP

Reactivity Notes

Immunogen displays the following percentage of sequence identity for non-tested species: Mouse (89%), Rat (89%).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

56 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for KLF4 Antibody - BSA Free

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940]

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940]

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940] - Staining in human skin and pancreas tissues. Corresponding KLF4 RNA-seq data are presented for the same tissues.
Western Blot: KLF4 Antibody [NBP1-83940]

Western Blot: KLF4 Antibody [NBP1-83940]

KLF4-Antibody-Western-Blot-NBP1-83940-img0025.jpg
Immunocytochemistry/ Immunofluorescence: KLF4 Antibody [NBP1-83940]

Immunocytochemistry/ Immunofluorescence: KLF4 Antibody [NBP1-83940]

Immunocytochemistry/Immunofluorescence: KLF4 Antibody [NBP1-83940] - Staining of human cell line A-431 shows localization to nucleoplasm. Antibody staining is shown in green.
Western Blot: KLF4 Antibody [NBP1-83940]

Western Blot: KLF4 Antibody [NBP1-83940]

Western Blot: KLF4 Antibody [NBP1-83940] - Human breast cancer cell MCF-7 was treated with carboplatin, alone or in combination with Stat3 inhibitor, for 72 hours, and the expression of Klf4 was detected by Western blot. WB image submitted by a verified customer review.
Western Blot: KLF4 Antibody [NBP1-83940]

Western Blot: KLF4 Antibody [NBP1-83940]

Western Blot: KLF4 Antibody [NBP1-83940] - Analysis in control (vector only transfected HEK293T lysate) and KLF4 over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Western Blot: KLF4 Antibody [NBP1-83940]

Western Blot: KLF4 Antibody [NBP1-83940]

Western Blot: KLF4 Antibody [NBP1-83940] - MDA-MB-231 subclones transfected with NTC or HIF-1 alpha shRNA vector were treated with vehicle (V) or paclitaxel (P) and immunoblot assay was performed. WB image submitted by a verified customer review.
Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940]

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940]

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940] - Staining of human colon shows moderate to strong nuclear positivity in glandular cells.
Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940]

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940]

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940] - Staining of human pancreas shows no positivity in exocrine glandular cells as expected.
Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940]

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940]

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940] - Staining of human skin shows moderate to strong nuclear positivity in keratinocytes.
Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940]

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940]

Immunohistochemistry-Paraffin: KLF4 Antibody [NBP1-83940] - Staining of human testis shows moderate to strong nuclear positivity in a subset of cells in seminiferous ducts.
KLF4 Antibody

Western Blot: KLF4 Antibody [NBP1-83940] -

Western Blot: KLF4 Antibody [NBP1-83940] - HIFs are required for hypoxia-induced expression of pluripotency factorsA-C. Breast cancer cell lines were exposed to 20% or 1% O2 for 24 h & NANOG (A), KLF4 (B), & SOX2 (C) mRNA levels were determined by RT-qPCR, relative to 18S rRNA, & normalized to the mean value for MDA-MB-231 cells (MDA231) at 20% O2 (mean ± SEM; n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. same cell line at 20% O2 by Student's t test. D & E. HCC-1954 (D) & MCF-7 (E) subclones, which were stably transfected with an expression vector encoding a non-targeting control (NTC) shRNA, or vector encoding shRNA targeting HIF-1 alpha (sh1 alpha ) or HIF-2 alpha (sh2 alpha ), or vectors encoding shRNAs targeting both HIF-1 alpha & HIF-2 alpha (DKD), were exposed to 20% or 1% O2 for 24 h & RT-qPCR was performed to determine NANOG (D) or KLF4 (E) mRNA levels relative to 18S rRNA. The results were normalized to NTC at 20% O2 (mean ± SEM; n = 3). *P < 0.05, **P < 0.01, ***P < 0.001 vs. NTC at 20% O2; #P < 0.05, ##P < 0.01, ###P < 0.001 vs. NTC at 1% O2 by ANOVA. F. ZR75.1 cells treated with vehicle or digoxin (200 nM) were exposed to 20% or 1% O2 for 24 h & SOX2 mRNA was measured (mean ± SEM; n = 3). *P < 0.05, **P < 0.01 vs. NTC at 20% O2; ###P < 0.001 vs. NTC at 1% O2 by ANOVA. G & H. NTC & DKD subclones of HCC-1954 (G) & MCF-7 (H) were exposed to 20% or 1% O2 for 48 h, whole cell lysates were prepared, & immunoblot assays were performed to analyze HIF-1 alpha, HIF-2 alpha, NANOG & KLF4 protein expression. Actin was also analyzed as a loading control. I. ZR75.1 cells were treated with vehicle or digoxin (200 nM), exposed to 20% or 1% O2 for 48 h, & HIF-1 alpha, NANOG & SOX2 immunoblot assays were performed. Image collected & cropped by CiteAb from the following publication (https://www.oncotarget.com/lookup/doi/10.18632/oncotarget.11743), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
KLF4 Antibody - BSA Free Western Blot: KLF4 Antibody - BSA Free [NBP1-83940]

Western Blot: KLF4 Antibody - BSA Free [NBP1-83940]

Analysis in human cell lines PC-3 and U2OS using Anti-KLF4 antibody. Corresponding KLF4 RNA-seq data are presented for the same cell lines. Loading control: Anti-GAPDH.
KLF4 Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: KLF4 Antibody - BSA Free [NBP1-83940]

Chromatin Immunoprecipitation-exo-Seq: KLF4 Antibody - BSA Free [NBP1-83940]

ChIP-Exo-Seq composite graph for Anti-KLF4 (NBP1-83940) tested in NCCIT cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.
KLF4 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: KLF4 Antibody - BSA Free [NBP1-83940] -

miR-7 regulate the expression of YY1 and KLF4 by binding its 3`UTR region. (A) Analysis of miR-7 binding sites in the 3`UTR regions of KLF4 and YY1, through the miRtarbase database. (B) Raji cell line was previously co-transfected with an inhibitor (INH) or a mimic (MIM) of miR-7, and then transfected with the reporter plasmid with the luciferase gene containing the 3 ‘UTR region of YY1 (B) and KLF4 (C). The presence of INH for miR-7 increases the expression of KLF4 and YY1 (***p < 0.001) and the MIM inhibits its expression (**p < 0.001 *p < 0.05), respectively. (D) Left: Western blot for KLF4 and YY1 was done, after treatment with an inhibitor or miR-7 mimic compared to a control. Right: densitometric analysis of YY1 and KLF4 expression was done and significant differences were indicated (*p < 0.05). (E) Immunofluorescence in Raji cell lines was done and expression of KLF4 and YY1 were evaluated after transfection with an inhibitor or miR-7 mimic. Representative images of a triplicate of each experiment are shown. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/33194748), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for KLF4 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:1000 - 1:2500

Immunohistochemistry-Paraffin

1:1000 - 1:2500

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF, fixation/permeabilization: PFA/Triton X-100.

Reviewed Applications

Read 2 reviews rated 4 using NBP1-83940 in the following applications:

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: KLF4

KLF4 is a transcription factor that works with Sp1 to activate the Laminin gamma1 chain gene. It binds the CACCC core sequence. KLF4 may be involved in the differentiation of epithelial cells and may also function in the development of the skeleton and kidney. KLF4 also has roles as both an oncogene and a tumor suppressor.

Long Name

Kruppel-Like Factor 4

Alternate Names

EZF

Entrez Gene IDs

9314 (Human)

Gene Symbol

KLF4

UniProt

O43474 (Human)

Additional KLF4 Products

Product Documents for KLF4 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for KLF4 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for KLF4 Antibody - BSA Free

Customer Reviews for KLF4 Antibody - BSA Free (2)

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Showing  1 - 2 of 2 reviews Showing All
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  • KLF4 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: Breast cancer cells
    Species: Human
    Verified Customer | Posted 05/18/2020
    MDA-MB-231 subclones transfected with NTC or HIF-1 alpha shRNA vector were treated with vehicle (V) or paclitaxel (P) and immunoblot assay was performed.
    KLF4 Antibody - BSA Free NBP1-83940
  • KLF4 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: breast cancer MCF7 cells
    Species: Human
    Verified Customer | Posted 09/21/2018
    Human breast cancer cell MCF-7 was treated with carboplatin, alone or in combination with Stat3 inhibitor, for 72 hours, and the expression of Klf4 was detected by western blot.
    KLF4 Antibody - BSA Free NBP1-83940

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