Klotho Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: Klotho Antibody - BSA Free [NBP1-76511] -

Immunocytochemistry/ Immunofluorescence: Klotho Antibody - BSA Free [NBP1-76511] - Figure 5 Immunofluorescence Validation of Klotho in Mouse Heart TissueImmunofluorescent analysis of 4% paraformaldehyde-fixed mouse heart tissue labeling Klotho with at 20 u/mL, followed by goat anti-rabbit IgG secondary antibody at 1/500 dilution (red).

Western Blot: Klotho AntibodyBSA Free [NBP1-76511]

Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Validation in Rat Kidney Tissue LysateLoading: 15ug of lysates per lane.Antibodies: KLOTHO 6107 (1ug/mL), 1h incubation at RT in 5% NFDM/TBST.Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

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Immunohistochemistry: Klotho Antibody - BSA Free [NBP1-76511]

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Western Blot: Klotho AntibodyBSA Free [NBP1-76511]

Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Independent Antibody Validation (IAV) via Protein Expression Profile in Human and Mouse Cell Lines. Loading: 15 ug of lysates per lane. Antibodies: KLOTHO NBP1-76511 (2 ug/mL), KLOTHO, competitor antibody (4 ug/mL) and beta-actin (1 ug/mL), 1h incubation at RT in 5% NFDM/TBST. Secondary: Goat anti-rabbit IgG HRP conjugate at 1:10000 dilution.

Western Blot: Klotho Antibody - BSA Free [NBP1-76511] -

Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - Blockade of the HIF‐1 alpha /p53/miRNA‐34a/Klotho axis decreases the leakage & area of mouse laser‐induced CNV. The mice were divided into the following groups: normal, CNV 7 d, CNV 7 d + 0.1% DMSO, CNV 7 d + digoxin (oral; 2 mg/kg for 7 d), CNV 7 d + AAV‐p53 mutant (intravitreal injection; approximately 3 μL, 3 × 1010 viral particles/mL), CNV 7 d + miRNA‐34a inhibitor (intravitreal injection; 1 μg) & CNV 7 d + AAV‐Klotho full‐length plasmid (intravitreal injection, 2 μL, 5 × 1010 viral particles/mL). A, Western blot was performed to measure HIF‐1 alpha, p‐p53 (S15), p53 (S20), p‐p53 (S46), p53 & Klotho protein levels. B, The relative protein levels of HIF‐1 alpha /GAPDH (B), p‐p53 (S15)/p53 (C), p‐p53 (S20)/p53 (D), p‐p53 (S46)/p53 (E), p53/GAPDH (F) & Klotho/GAPDH (G) were analysed. ***P < .001, CNV 7‐d group vs normal group. ##P < .01, compared with the CNV 7‐d group. NS, CNV 7 d + KL OE group vs CNV 7‐d group. H, RT‐PCR was performed to measure the expression of miRNA‐34a. **P < .01, CNV 7‐d group vs normal group. ##P < .01, compared with the CNV 7‐d group. NS, CNV 7 d + KL OE group vs CNV 7‐d group. I, FFA was performed to measure the leakage of CNV. J, The leakage of CNV was analysed. K, ICGA was performed to measure the area of CNV. L, The area of CNV was analysed. **P < .01, ***P < .001, compared with the CNV 7‐d group Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33438362), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Klotho Antibody - BSA Free [NBP1-76511] -

Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - microrna‐34a inhibits the expression of Klotho. ARPE‐19 cells were divided into the following groups: normal, hypoxia, hypoxia + AAV vector, hypoxia + digoxin, hypoxia + digoxin + nutlin‐3a, hypoxia + AAV‐p53 mutant infection & hypoxia + miRNA‐34a inhibitor. A, Western blot assay of Klotho in ARPE‐19 cells was performed. B, The relative protein level of Klotho compared with the GAPDH level was analysed. ***P < .001, hypoxia group vs normal group. ###P < .001, ##P < .01, compared with the hypoxia group. %%P < .01, hypoxia + digoxin + nutlin‐3a group vs hypoxia + digoxin group. C, Schematic of the Klotho 3' UTR with the WT or mutated putative‐binding site of miRNA‐34a inserted into a luciferase (Luc) reporter. D, qRT‐PCR analysis of Klotho mRNA expression in HEK293T cells transfected with miRNA‐34a mimic for 24 h. **P < .01, miRNA‐34a mimic group vs control group. E, Western blot of Klotho in HEK293T cells transfected with a miRNA‐34a mimic for 24 h was performed. F, Immunoblotted Klotho was quantified & normalized to GAPDH. ***P < .001, miRNA‐34a mimic group vs control group. G Relative luciferase activity was detected in HEK293T cells co‐transfected with plasmids containing firefly luciferase & wild‐type (WT) or mutant (Mut) Klotho 3′‐UTR & miRNA‐34a mimic for 24 h. The luciferase activity values were normalized to Renilla reniformis luciferase (TK‐RL) activity. **P < .01, Luc‐Klotho 3′ UTR WT group vs vector group. Statistically non‐significant (NS), Luc‐Klotho 3′ UTR mutant group vs vector group Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/33438362), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Western Blot: Klotho Antibody - BSA Free [NBP1-76511] -

Western Blot: Klotho Antibody - BSA Free [NBP1-76511] - High phytate-induced renal phosphate wasting is independent of FGF23.(A) The renal expression of downregulated genes associated with Ca2+ & phosphate homeostasis in rats fed control or phytate-supplemented diets (n = 8 per group). (B) Results of immunoblot analyses & protein levels of renal aKlotho, NHERF1, & NaPi-2a after 2 weeks for rats fed control, HP- LCa2+, & HP-HCa2+ diets. (C) The levels of renal proteins were quantified using ImageJ software (n = 6 per group). (D, E) Time course analysis of serum levels of intact FGF23 (D) & C-terminal FGF23 (E) in rats fed control, HP-LCa2+, & HP-HCa2+ diets. (F) Representative immunohistochemical staining of EDTA-decalcified femur sections for FGF23 from rats fed control, HP-LCa2+, & HP-HCa2+ diets. All data are presented as the mean ± SD of each group (n = 3–8 per group). *, p<0.05; **, p<0.01; ***, p<0.001, compared with controls. Image collected & cropped by CiteAb from the following publication (https://pubmed.ncbi.nlm.nih.gov/32271147), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Immunohistochemistry-Paraffin: Klotho Antibody - BSA Free [NBP1-76511] -

WD feeding induced changes in FGF-23 and Klotho expression are restored by DPP-4 inhibition. a FGF-23 staining; b Endothelial FGF-23; c Adventitia FGF-23; d Endothelial klotho staining; e Endothelial klotho staining f Adventitia klotho staining; Average gray intensities in the different cohorts. Values are mean +/- SE. CDC control diet control, CDL control diet linagliptin, WDC western diet control and WDL western diet linagliptin. Post-hoc comparisons within a time point; *p < 0 0.05 CDC vs WDC; †p < 0.05 WDC vs WDL; #p < 0.05 CDC vs CDL. Scale bars represent 50 mμ Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/27391040), licensed under a CC-BY license. Not internally tested by Novus Biologicals.