Leptin/OB Antibody

Novus Biologicals | Catalog # NB300-611

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Porcine, Avian, Sheep

Applications

Immunohistochemistry, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Leptin/OB Primary Antibodies »

Product Specifications

Immunogen

Synthetic peptide corresponding to residues Q(25) K V Q D D T K T L I K T I V T R I N D(44) of mouse Leptin.

Reactivity Notes

Rat reactivity reported in scientific literature (PMID: 23270329). Human reactivity reported in scientific literature (PMID: 21983012). Porcine reactivity reported in scientific literature (PMID: 18413648). Avian reactivity reported in scientific literature (PMID: 16730725).

Localization

Secreted

Specificity

Leptin

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Leptin/OB Antibody

Western Blot: Leptin/OB Antibody [NB300-611]

Western Blot: Leptin/OB Antibody [NB300-611]

Western Blot: Leptin/OB Antibody [NB300-611] - Analysis of leptin in mouse 3T3-L1.
Immunohistochemistry-Frozen: Leptin/OB Antibody [NB300-611]

Immunohistochemistry-Frozen: Leptin/OB Antibody [NB300-611]

Immunohistochemistry-Frozen: Leptin/OB Antibody [NB300-611] - Staining of Leptin in sheep brain.

Applications for Leptin/OB Antibody

Application
Recommended Usage

Immunocytochemistry

1:50 - 1:200

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Frozen

1:50 - 1:200

Western Blot

1:200 - 1:1000
Application Notes
WB: Detects an approx. 16 kDa protein representing leptin from 3T3-L1 cell extract. IHC: Staining of leptin in sheep brain with NB300-611 results in staining of the ventromedial hypothalamus.

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS with 1 mg/ml BSA

Preservative

0.05% Sodium Azide

Concentration

2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Leptin/OB

Leptin, the obese (ob) gene product, is a small protein expressed in and secreted from adipose tissue of normal rodents. Studies suggest leptin acts as a circulating hormone capable of regulating body-weight homeostasis and energy balance. One possible target tissue for leptin is the hypothalamus, a proposed control center for satiety and energy expenditure. Ob gene knockout mice are characterized by several metabolic abnormalities including hyperglucocorticoidemia, hyperinsulinemia and insulin resistance, hyperglycemia, altered central nervous system activity, reduced metabolic rate of brown adipose tissue, and a large increase in white adipose tissue. Studies show that the administration of recombinant leptin to ob knockout mice reduces food intake and increases energy expenditure.

Alternate Names

LEP, OB

Entrez Gene IDs

3952 (Human); 16846 (Mouse); 25608 (Rat)

Gene Symbol

LEP

UniProt

P41160 (Mouse)

Additional Leptin/OB Products

Product Documents for Leptin/OB Antibody

Certificate of Analysis

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Product Specific Notices for Leptin/OB Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for Leptin/OB Antibody

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Protocols

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