Human Total MMP-3 Quantikine ELISA Kit DMP300: R&D Systems

Human Total MMP-3 Quantikine ELISA Kit

(30 citations)
(1 Review)
  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL)
  • Sensitivity
    0.045 ng/mL
  • Assay Range
    0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
  • Specificity
    Natural and recombinant human total MMP-3
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC127, Quantikine Immunoassay Control Set 637 for Human Total MMP-3 - Please Inquire
Product Summary
The Quantikine Human Total MMP-3 Immunoassay is a 4.5 hour solid phase immunoassay designed to measure total MMP-3 (pro- and active MMP-3) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Pro-MMP-3 and antibodies raised against the recombinant factor. Both antibodies also recognize recombinant human active MMP-3. Natural human MMP-3 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human MMP-3.

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.
Cell Culture Supernates, Serum, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Standard Deviation0.0620.1970.3320.0850.2170.449


The recovery of MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 87-104
Heparin Plasma (n=5) 92 85-106
Serum (n=5) 90 86-96
To assess the linearity of the assay, samples containing high concentrations of MMP-3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
Human Total MMP-3 Quantikine ELISA Kit
Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MMP-3

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

    • Long Name
      Matrix Metalloproteinase 3
    • Entrez Gene IDs
      4314 (Human); 17392 (Mouse);
    • Alternate Names
      CHDS6; EC 3.4.24; EC; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 (stromelysin 1, progelatinase); Matrix metalloproteinase-3; MGC126102; MGC126103; MMP3; MMP-3; proteoglycanase; SL-1; STMY; STMY1MGC126104; STR1; Stromelysin 1; stromelysin-1; transin-1;
    Related Research Areas
    Assay Procedure
    Refer to the product for complete assay procedure.

    Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
    1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
    2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

    3. 100 µL Assay Diluent
    4.   Add 100 µL of Assay Diluent to each well.

    5. 100 µL Standard, Control, or Sample
    6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
    7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

    8. 200 µL Conjugate
    9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
    10.   Aspirate and wash 4 times.

    11. 200 µL Substrate Solution
    12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

    13. 50 µL Stop Solution
    14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

    R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

    30 Citations: Showing 1 - 10
    Filter your results:

    Sample Type
    1. Exosomes in Human Breast Milk Promote EMT
      Authors: W Qin, Y Tsukasaki, S Dasgupta, N Mukhopadhy, M Ikebe, ER Sauter
      Clin Cancer Res, 2016;0(0):.
      Species: Human
      Sample Type: Cell Lysates
    2. Galectin-3 Induces a Pro-degradative/inflammatory Gene Signature in Human Chondrocytes, Teaming Up with Galectin-1 in Osteoarthritis Pathogenesis
      Sci Rep, 2016;6(0):39112.
      Species: Human
      Sample Type: Cell Culture Supernates
    3. Concomitant elevations of MMP-9, NGAL, proMMP-9/NGAL and neutrophil elastase in serum of smokers with chronic obstructive pulmonary disease
      J. Cell. Mol. Med, 2016;0(0):.
      Species: Human
      Sample Type: Serum
    4. Metallothionein-3 Increases Triple-Negative Breast Cancer Cell Invasiveness via Induction of Metalloproteinase Expression.
      Authors: Kmiecik A, Pula B, Suchanski J, Olbromski M, Gomulkiewicz A, Owczarek T, Kruczak A, Ambicka A, Rys J, Ugorski M, Podhorska-Okolow M, Dziegiel P
      PLoS ONE, 2015;10(5):e0124865.
      Species: Human
      Sample Type: Cell Culture Supernates
    5. Exogenous IFN-beta regulates the RANKL-c-Fos-IFN-beta signaling pathway in the collagen antibody-induced arthritis model.
      Authors: Zhao R, Chen N, Zhou X, Miao P, Hu C, Qian L, Yu Q, Zhang J, Nie H, Chen X, Li P, Xu R, Xiao L, Zhang X, Liu J, Zhang D
      J Transl Med, 2014;12(1):330.
      Species: Human
      Sample Type: Serum
    6. Interleukin-6 and matrix metalloproteinase expression in the subretinal fluid during proliferative vitreoretinopathy: correlation with extent, duration of RRD and PVR grade.
      Authors: Symeonidis C, Papakonstantinou E, Androudi S, Tsaousis KT, Tsinopoulos I, Brazitikos P, Karakiulakis G, Diza E, Dimitrakos SA
      Cytokine, 2012;59(1):184-90.
      Species: Human
      Sample Type: Subretinal Fluid
    7. Validation and quality control of ELISAs for the use with human saliva samples.
      Authors: Jaedicke KM, Taylor JJ, Preshaw PM
      J. Immunol. Methods, 2012;377(1):62-5.
      Species: Human
      Sample Type: Saliva
    8. Interleukin-6 and the matrix metalloproteinase response in the vitreous during proliferative vitreoretinopathy.
      Authors: Symeonidis C, Papakonstantinou E, Androudi S, Rotsos T, Diza E, Brazitikos P, Karakiulakis G, Dimitrakos SA
      Cytokine, 2011;54(2):212-7.
      Species: Human
      Sample Type: Vitreous Humor
    9. Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells.
      Authors: Yamakawa N, Uchida T, Matthay MA, Makita K
      Am. J. Physiol. Lung Cell Mol. Physiol., 2011;300(4):L516-25.
      Species: Human
      Sample Type: Edema Fluid
    10. Cyclooxygenase-2 inhibitor blocks the production of West Nile virus-induced neuroinflammatory markers in astrocytes.
      Authors: Verma S, Kumar M, Nerurkar VR
      J. Gen. Virol., 2011;92(0):507-15.
      Species: Human
      Sample Type: Cell Culture Supernates
    11. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
      Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
      Eur. J. Haematol., 2010;84(3):239-51.
      Species: Human
      Sample Type: Cell Culture Supernates
    12. Matrix metalloproteinase-9 and plasminogen activator inhibitor-1 are associated with right ventricular structure and function: the MESA-RV Study.
      Authors: Kawut SM, Barr RG, Johnson WC
      Biomarkers, 2010;15(8):731-8.
      Species: Human
      Sample Type: Plasma
    13. Soluble biomarkers of cartilage and bone metabolism in early proof of concept trials in psoriatic arthritis: effects of adalimumab versus placebo.
      Authors: van Kuijk AW, DeGroot J, Koeman RC
      PLoS ONE, 2010;5(9):e12556.
      Species: Human
      Sample Type: Serum
    14. Elevated serum matrix metalloproteinase-3 and -7 in H. pylori-related gastric cancer can be biomarkers correlating with a poor survival.
      Authors: Yeh YC, Sheu BS, Cheng HC, Wang YL, Yang HB, Wu JJ
      Dig. Dis. Sci., 2010;55(6):1649-57.
      Species: Human
      Sample Type: Serum
    15. Circulating matrix metalloproteinase-3 and metalloproteinase-9 and tissue Doppler measures of diastolic dysfunction to risk stratify patients with systolic heart failure.
      Authors: Buralli S, Dini FL, Ballo P, Conti U, Fontanive P, Duranti E, Metelli MR, Marzilli M, Taddei S
      Am. J. Cardiol., 2010;105(6):853-6.
      Species: Human
      Sample Type: Plasma
    16. High molecular weight hyaluronic acid inhibits IL-6-induced MMP production from human chondrocytes by up-regulating the ERK inhibitor, MKP-1.
      Authors: Hashizume M, Mihara M
      Biochem. Biophys. Res. Commun., 2010;403(2):184-9.
      Species: Human
      Sample Type: Cell Culture Supernates
    17. Serum levels of matrix metalloproteinases -1,-2,-3 and -9 in thoracic aortic diseases and acute myocardial ischemia.
      Authors: Karapanagiotidis GT, Antonitsis P, Charokopos N, Foroulis CN, Anastasiadis K, Rouska E, Argiriadou H, Rammos K, Papakonstantinou C
      J Cardiothorac Surg, 2009;4(0):59.
      Species: Human
      Sample Type: Serum
    18. Gender specific associations between matrix metalloproteinases and inflammatory markers in post myocardial infarction patients.
      Authors: Samnegard A, Hulthe J, Silveira A, Ericsson CG, Hamsten A, Eriksson P
      Atherosclerosis, 2009;202(2):550-6.
      Species: Human
      Sample Type: Serum
    19. PI3Kgamma regulates cartilage damage in chronic inflammatory arthritis.
      Authors: Hayer S, Pundt N, Peters MA, Wunrau C, Kuhnel I, Neugebauer K, Strietholt S, Zwerina J, Korb A, Penninger J, Joosten LA, Gay S, Ruckle T, Schett G, Pap T
      FASEB J., 2009;23(12):4288-98.
      Species: Human
      Sample Type: Cell Culture Supernates
    20. Inhibition of IL-1beta-mediated inflammatory responses by the IkappaB alpha super-repressor in human fibroblast-like synoviocytes.
      Authors: Lee YR, Kweon SH, Kwon KB, Park JW, Yoon TR, Park BH
      Biochem. Biophys. Res. Commun., 2009;378(1):90-4.
      Species: Human
      Sample Type: Cell Culture Supernates
    21. Establishment of a matrix-associated transepithelial resistance invasion assay to precisely measure the invasive potential of synovial fibroblasts.
      Authors: Wunrau C, Schnaeker EM, Freyth K, Pundt N, Wendholt D, Neugebauer K, Hansen U, Pap T, Dankbar B
      Arthritis Rheum., 2009;60(9):2606-11.
      Species: Human
      Sample Type: Cell Culture Supernates
    22. Targeting a metalloprotease-PAR1 signaling system with cell-penetrating pepducins inhibits angiogenesis, ascites, and progression of ovarian cancer.
      Authors: Agarwal A, Covic L, Sevigny LM, Kaneider NC, Lazarides K, Azabdaftari G, Sharifi S, Kuliopulos A
      Mol. Cancer Ther., 2008;7(9):2746-57.
      Species: Human
      Sample Type: Ovarian Ascites Fluid
    23. Serum levels of MMP-3 and cathepsin K in patients with ankylosing spondylitis: effect of TNFalpha antagonist therapy.
      Authors: Wendling D, Cedoz JP, Racadot E
      Joint Bone Spine, 2008;75(5):559-62.
      Species: Human
      Sample Type: Serum
    24. Actinobacillus actinomycetemcomitans lipopolysaccharide regulates matrix metalloproteinase, tissue inhibitors of matrix metalloproteinase, and plasminogen activator production by human gingival fibroblasts: a potential role in connective tissue destruction.
      Authors: Bodet C, Andrian E, Tanabe S, Grenier D
      J. Cell. Physiol., 2007;212(1):189-94.
      Species: Human
      Sample Type: Cell Culture Supernates
    25. Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.
      Authors: Zhao Z, McCloud B, Fleming R, Klempner MS
      Biochem. Biophys. Res. Commun., 2007;358(2):528-33.
      Species: Human
      Sample Type: Tissue Homogenates
    26. Advanced glycation end products decrease mesangial cell MMP-7: a role in matrix accumulation in diabetic nephropathy?
      Authors: McLennan SV, Kelly DJ, Schache M, Waltham M, Dy V, Langham RG, Yue DK, Gilbert RE
      Kidney Int., 2007;72(4):481-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    27. Gingival fibroblasts inhibit MMP-1 and MMP-3 activities in an ex-vivo artery model.
      Authors: Naveau A, Reinald N, Fournier B
      Connect. Tissue Res., 2007;48(6):300-8.
      Species: Human
      Sample Type: Cell Culture Supernates
    28. Advanced glycation end products increases matrix metalloproteinase-1, -3, and -13, and TNF-alpha in human osteoarthritic chondrocytes.
      Authors: Nah SS, Choi IY, Yoo B, Kim YG, Moon HB, Lee CK
      FEBS Lett., 2007;581(9):1928-32.
      Species: Human
      Sample Type: Cell Culture Supernates
    29. Stimulation of matrix metalloproteinases by hyperosmolarity via a JNK pathway in human corneal epithelial cells.
      Authors: Li DQ, Chen Z, Song XJ, Luo L, Pflugfelder SC
      Invest. Ophthalmol. Vis. Sci., 2004;45(12):4302-11.
      Species: Human
      Sample Type: Cell Culture Supernates
    30. Antidiabetic PPAR gamma-activator rosiglitazone reduces MMP-9 serum levels in type 2 diabetic patients with coronary artery disease.
      Authors: Marx N, Froehlich J, Siam L, Ittner J, Wierse G, Schmidt A, Scharnagl H, Hombach V, Koenig W
      Arterioscler. Thromb. Vasc. Biol., 2003;23(2):283-8.
      Species: Human
      Sample Type: Serum
    Expand to show all 30 Citations
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