Human Total MMP-3 Quantikine ELISA Kit

  (34 citations)
(1 Review)
    
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Product Details
Assay Procedure
Citations (34)
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  • Assay Type
    Solid Phase Sandwich ELISA
  • Format
    96-well strip plate
  • Assay Length
    4.5 hours
  • Sample Type & Volume Required Per Well
    Cell Culture Supernates (50 uL), Serum (10 uL), Heparin Plasma (10 uL)
  • Sensitivity
    0.045 ng/mL
  • Assay Range
    0.2 - 10 ng/mL (Cell Culture Supernates, Serum, Heparin Plasma)
  • Specificity
    Natural and recombinant human total MMP-3
  • Cross-reactivity
    Cross-reactivity observed with 1 or more available related molecules.Cross-species reactivity not tested.
  • Interference
    No significant interference observed with available related molecules.
Control Available
QC127 , Quantikine Immunoassay Control Set 637 for Human Total MMP-3 - Please Inquire
Product Summary
The Quantikine Human Total MMP-3 Immunoassay is a 4.5 hour solid phase immunoassay designed to measure total MMP-3 (pro- and active MMP-3) in cell culture supernates, serum, and plasma. It contains NS0-expressed recombinant human Pro-MMP-3 and antibodies raised against the recombinant factor. Both antibodies also recognize recombinant human active MMP-3. Natural human MMP-3 showed dose-response curves that were parallel to the standard curves obtained using the recombinant Quantikine kit standards, indicating that this kit can be used to determine relative levels of natural human MMP-3.

Precision
Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision
Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision
Cell Culture Supernates, Serum, Heparin Plasma
Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 40 40 40
Mean 0.962 3.21 5.87 0.994 3.08 5.7
Standard Deviation 0.062 0.197 0.332 0.085 0.217 0.449
CV% 6.4 6.1 5.7 8.6 7 7.9

Recovery

The recovery of MMP-3 spiked to levels throughout the range of the assay in various matrices was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 96 87-104
Heparin Plasma (n=5) 92 85-106
Serum (n=5) 90 86-96
Linearity
To assess the linearity of the assay, samples containing high concentrations of MMP-3 were serially diluted with Calibrator Diluent to produce samples with values within the dynamic range of the assay.
 MMP-3 [HRP]
Product Datasheets

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Preparation and Storage
  • Storage
    Store the unopened product at 2 - 8 °C. Do not use past expiration date.
Background: MMP-3

The matrix metalloproteinases (MMPs) consist of 24 known human zinc proteases with essential roles in breaking down components of the extracellular matrix (ECM). Additional MMP substrates include cytokines, chemokines, growth factors and binding proteins, cell/cell adhesion molecules, and other proteinases. With a few exceptions, MMPs share common structural motifs including a pro-peptide domain, a catalytic domain, a hinge region, and a hemopexin-like domain. Synthesized as pro-enzymes, most MMPs are secreted before conversion to their active form. MMP activities are modulated on several levels including transcription, pro-enzyme activation, or by their endogenous inhibitors, tissue inhibitors of metalloproteinases (TIMPs). A subset of MMPs are associated with membranes and designated as membrane-type metalloproteinases (MT-MMP).

  • Long Name:
    Matrix Metalloproteinase 3
  • Entrez Gene IDs:
    4314 (Human); 17392 (Mouse)
  • Alternate Names:
    CHDS6; EC 3.4.24; EC 3.4.24.17; matrix metallopeptidase 3 (stromelysin 1, progelatinase); matrix metalloproteinase 3 (stromelysin 1, progelatinase); Matrix metalloproteinase-3; MGC126102; MGC126103; MMP3; MMP-3; proteoglycanase; SL-1; STMY; STMY1MGC126104; STR1; Stromelysin 1; stromelysin-1; transin-1
Related Research Areas
Assay Procedure
Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.

  3. 100 µL Assay Diluent
  4.   Add 100 µL of Assay Diluent to each well.

  5. 100 µL Standard, Control, or Sample
  6.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  7.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.

  8. 200 µL Conjugate
  9.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  10.   Aspirate and wash 4 times.

  11. 200 µL Substrate Solution
  12.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.

  13. 50 µL Stop Solution
  14.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

34 Citations: Showing 1 - 10
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Species
Sample Type
  1. PhosphoLipid transfer protein (PLTP) exerts a direct pro-inflammatory effect on rheumatoid arthritis (RA) fibroblasts-like-synoviocytes (FLS) independently of its lipid transfer activity
    Authors: R Audo, V Deckert, CI Daien, H Che, J Elhmioui, S Lemaire, JP Pais de Ba, C Desrumaux, B Combe, M Hahne, L Lagrost, J Morel
    PLoS ONE, 2018;13(3):e0193815.
    Species: Human
    Sample Type: Cell Culture Supernates
  2. Late mitral restenosis after percutaneous commissurotomy: Predictive value of inflammation and extracellular matrix remodeling biomarkers
    Authors: R Mechmeche, A Zaroui, S Aloui, M Boukhris, M Allal-Elas, N Kaabachi, B Zouari
    Heart Lung, 2017;0(0):.
    Species: Human
    Sample Type: Serum
  3. CX3CL1 promotes MMP-3 production via the CX3CR1, c-Raf, MEK, ERK, and NF-?B signaling pathway in osteoarthritis synovial fibroblasts
    Authors: SM Hou, CH Hou, JF Liu
    Arthritis Res. Ther., 2017;19(1):282.
    Species: Human
    Sample Type: Cells Culture Supernates
  4. Serum bone-turnover biomarkers are associated with the occurrence of peripheral and axial arthritis in psoriatic disease: a prospective cross-sectional comparative study
    Authors: DR Jadon, R Sengupta, A Nightingal, H Lu, J Dunphy, A Green, JT Elder, RP Nair, E Korendowyc, MA Lindsay, NJ McHugh
    Arthritis Res. Ther., 2017;19(1):210.
    Species: Human
    Sample Type: Serum
  5. Galectin-3 Induces a Pro-degradative/inflammatory Gene Signature in Human Chondrocytes, Teaming Up with Galectin-1 in Osteoarthritis Pathogenesis
    Sci Rep, 2016;6(0):39112.
    Species: Human
    Sample Type: Cell Culture Supernates
  6. Concomitant elevations of MMP-9, NGAL, proMMP-9/NGAL and neutrophil elastase in serum of smokers with chronic obstructive pulmonary disease
    J. Cell. Mol. Med, 2016;0(0):.
    Species: Human
    Sample Type: Serum
  7. Exosomes in Human Breast Milk Promote EMT
    Authors: W Qin, Y Tsukasaki, S Dasgupta, N Mukhopadhy, M Ikebe, ER Sauter
    Clin Cancer Res, 2016;0(0):.
    Species: Human
    Sample Type: Cell Lysates
  8. Metallothionein-3 Increases Triple-Negative Breast Cancer Cell Invasiveness via Induction of Metalloproteinase Expression.
    Authors: Kmiecik A, Pula B, Suchanski J, Olbromski M, Gomulkiewicz A, Owczarek T, Kruczak A, Ambicka A, Rys J, Ugorski M, Podhorska-Okolow M, Dziegiel P
    PLoS ONE, 2015;10(5):e0124865.
    Species: Human
    Sample Type: Cell Culture Supernates
  9. Exogenous IFN-beta regulates the RANKL-c-Fos-IFN-beta signaling pathway in the collagen antibody-induced arthritis model.
    Authors: Zhao R, Chen N, Zhou X, Miao P, Hu C, Qian L, Yu Q, Zhang J, Nie H, Chen X, Li P, Xu R, Xiao L, Zhang X, Liu J, Zhang D
    J Transl Med, 2014;12(1):330.
    Species: Human
    Sample Type: Serum
  10. Validation and quality control of ELISAs for the use with human saliva samples.
    Authors: Jaedicke KM, Taylor JJ, Preshaw PM
    J. Immunol. Methods, 2012;377(1):62-5.
    Species: Human
    Sample Type: Saliva
  11. Interleukin-6 and matrix metalloproteinase expression in the subretinal fluid during proliferative vitreoretinopathy: correlation with extent, duration of RRD and PVR grade.
    Authors: Symeonidis C, Papakonstantinou E, Androudi S, Tsaousis KT, Tsinopoulos I, Brazitikos P, Karakiulakis G, Diza E, Dimitrakos SA
    Cytokine, 2012;59(1):184-90.
    Species: Human
    Sample Type: Subretinal Fluid
  12. Cyclooxygenase-2 inhibitor blocks the production of West Nile virus-induced neuroinflammatory markers in astrocytes.
    Authors: Verma S, Kumar M, Nerurkar VR
    J. Gen. Virol., 2011;92(0):507-15.
    Species: Human
    Sample Type: Cell Culture Supernates
  13. Interleukin-6 and the matrix metalloproteinase response in the vitreous during proliferative vitreoretinopathy.
    Authors: Symeonidis C, Papakonstantinou E, Androudi S, Rotsos T, Diza E, Brazitikos P, Karakiulakis G, Dimitrakos SA
    Cytokine, 2011;54(2):212-7.
    Species: Human
    Sample Type: Vitreous Humor
  14. Proteolytic release of the receptor for advanced glycation end products from in vitro and in situ alveolar epithelial cells.
    Authors: Yamakawa N, Uchida T, Matthay MA, Makita K
    Am. J. Physiol. Lung Cell Mol. Physiol., 2011;300(4):L516-25.
    Species: Human
    Sample Type: Edema Fluid
  15. Primary human acute myelogenous leukemia cells release matrix metalloproteases and their inhibitors: release profile and pharmacological modulation.
    Authors: Reikvam H, Hatfield KJ, Oyan AM, Kalland KH, Kittang AO, Bruserud O
    Eur. J. Haematol., 2010;84(3):239-51.
    Species: Human
    Sample Type: Cell Culture Supernates
  16. High molecular weight hyaluronic acid inhibits IL-6-induced MMP production from human chondrocytes by up-regulating the ERK inhibitor, MKP-1.
    Authors: Hashizume M, Mihara M
    Biochem. Biophys. Res. Commun., 2010;403(2):184-9.
    Species: Human
    Sample Type: Cell Culture Supernates
  17. Matrix metalloproteinase-9 and plasminogen activator inhibitor-1 are associated with right ventricular structure and function: the MESA-RV Study.
    Authors: Kawut SM, Barr RG, Johnson WC
    Biomarkers, 2010;15(8):731-8.
    Species: Human
    Sample Type: Plasma
  18. Circulating matrix metalloproteinase-3 and metalloproteinase-9 and tissue Doppler measures of diastolic dysfunction to risk stratify patients with systolic heart failure.
    Authors: Buralli S, Dini FL, Ballo P, Conti U, Fontanive P, Duranti E, Metelli MR, Marzilli M, Taddei S
    Am. J. Cardiol., 2010;105(6):853-6.
    Species: Human
    Sample Type: Plasma
  19. Elevated serum matrix metalloproteinase-3 and -7 in H. pylori-related gastric cancer can be biomarkers correlating with a poor survival.
    Authors: Yeh YC, Sheu BS, Cheng HC, Wang YL, Yang HB, Wu JJ
    Dig. Dis. Sci., 2010;55(6):1649-57.
    Species: Human
    Sample Type: Serum
  20. Soluble biomarkers of cartilage and bone metabolism in early proof of concept trials in psoriatic arthritis: effects of adalimumab versus placebo.
    Authors: van Kuijk AW, DeGroot J, Koeman RC
    PLoS ONE, 2010;5(9):e12556.
    Species: Human
    Sample Type: Serum
  21. Gender specific associations between matrix metalloproteinases and inflammatory markers in post myocardial infarction patients.
    Authors: Samnegard A, Hulthe J, Silveira A, Ericsson CG, Hamsten A, Eriksson P
    Atherosclerosis, 2009;202(2):550-6.
    Species: Human
    Sample Type: Serum
  22. Inhibition of IL-1beta-mediated inflammatory responses by the IkappaB alpha super-repressor in human fibroblast-like synoviocytes.
    Authors: Lee YR, Kweon SH, Kwon KB, Park JW, Yoon TR, Park BH
    Biochem. Biophys. Res. Commun., 2009;378(1):90-4.
    Species: Human
    Sample Type: Cell Culture Supernates
  23. PI3Kgamma regulates cartilage damage in chronic inflammatory arthritis.
    Authors: Hayer S, Pundt N, Peters MA, Wunrau C, Kuhnel I, Neugebauer K, Strietholt S, Zwerina J, Korb A, Penninger J, Joosten LA, Gay S, Ruckle T, Schett G, Pap T
    FASEB J., 2009;23(12):4288-98.
    Species: Human
    Sample Type: Cell Culture Supernates
  24. Establishment of a matrix-associated transepithelial resistance invasion assay to precisely measure the invasive potential of synovial fibroblasts.
    Authors: Wunrau C, Schnaeker EM, Freyth K, Pundt N, Wendholt D, Neugebauer K, Hansen U, Pap T, Dankbar B
    Arthritis Rheum., 2009;60(9):2606-11.
    Species: Human
    Sample Type: Cell Culture Supernates
  25. Serum levels of matrix metalloproteinases -1,-2,-3 and -9 in thoracic aortic diseases and acute myocardial ischemia.
    Authors: Karapanagiotidis GT, Antonitsis P, Charokopos N, Foroulis CN, Anastasiadis K, Rouska E, Argiriadou H, Rammos K, Papakonstantinou C
    J Cardiothorac Surg, 2009;4(0):59.
    Species: Human
    Sample Type: Serum
  26. Targeting a metalloprotease-PAR1 signaling system with cell-penetrating pepducins inhibits angiogenesis, ascites, and progression of ovarian cancer.
    Authors: Agarwal A, Covic L, Sevigny LM, Kaneider NC, Lazarides K, Azabdaftari G, Sharifi S, Kuliopulos A
    Mol. Cancer Ther., 2008;7(9):2746-57.
    Species: Human
    Sample Type: Ovarian Ascites Fluid
  27. Serum levels of MMP-3 and cathepsin K in patients with ankylosing spondylitis: effect of TNFalpha antagonist therapy.
    Authors: Wendling D, Cedoz JP, Racadot E
    Joint Bone Spine, 2008;75(5):559-62.
    Species: Human
    Sample Type: Serum
  28. Advanced glycation end products decrease mesangial cell MMP-7: a role in matrix accumulation in diabetic nephropathy?
    Authors: McLennan SV, Kelly DJ, Schache M, Waltham M, Dy V, Langham RG, Yue DK, Gilbert RE
    Kidney Int., 2007;72(4):481-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  29. Borrelia burgdorferi-induced monocyte chemoattractant protein-1 production in vivo and in vitro.
    Authors: Zhao Z, McCloud B, Fleming R, Klempner MS
    Biochem. Biophys. Res. Commun., 2007;358(2):528-33.
    Species: Human
    Sample Type: Tissue Homogenates
  30. Advanced glycation end products increases matrix metalloproteinase-1, -3, and -13, and TNF-alpha in human osteoarthritic chondrocytes.
    Authors: Nah SS, Choi IY, Yoo B, Kim YG, Moon HB, Lee CK
    FEBS Lett., 2007;581(9):1928-32.
    Species: Human
    Sample Type: Cell Culture Supernates
  31. Gingival fibroblasts inhibit MMP-1 and MMP-3 activities in an ex-vivo artery model.
    Authors: Naveau A, Reinald N, Fournier B
    Connect. Tissue Res., 2007;48(6):300-8.
    Species: Human
    Sample Type: Cell Culture Supernates
  32. Actinobacillus actinomycetemcomitans lipopolysaccharide regulates matrix metalloproteinase, tissue inhibitors of matrix metalloproteinase, and plasminogen activator production by human gingival fibroblasts: a potential role in connective tissue destruction.
    Authors: Bodet C, Andrian E, Tanabe S, Grenier D
    J. Cell. Physiol., 2007;212(1):189-94.
    Species: Human
    Sample Type: Cell Culture Supernates
  33. Stimulation of matrix metalloproteinases by hyperosmolarity via a JNK pathway in human corneal epithelial cells.
    Authors: Li DQ, Chen Z, Song XJ, Luo L, Pflugfelder SC
    Invest. Ophthalmol. Vis. Sci., 2004;45(12):4302-11.
    Species: Human
    Sample Type: Cell Culture Supernates
  34. Antidiabetic PPAR gamma-activator rosiglitazone reduces MMP-9 serum levels in type 2 diabetic patients with coronary artery disease.
    Authors: Marx N, Froehlich J, Siam L, Ittner J, Wierse G, Schmidt A, Scharnagl H, Hombach V, Koenig W
    Arterioscler. Thromb. Vasc. Biol., 2003;23(2):283-8.
    Species: Human
    Sample Type: Serum
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Quantikine Immunoassay Control Set 637 for Human Total MMP-3

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Average Rating: 4 (Based on 1 review)

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 Human Total MMP-3 Quantikine ELISA Kit DMP300 Array DMP300
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 Human Total MMP-3 Quantikine ELISA Kit DMP300
: Human Total MMP-3 Quantikine ELISA Kit [DMP300].

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