MagCellect Cell Selection Kits provide a powerful tool for purifying cell populations by negative selection.
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MagCellect is a great choice when a population of pure and “untouched” cells is required. For example, eosinophils, T cells, and other populations often become “activated” upon binding to antibody magnetic beads. Because MagCellect Cell Selection kits use negative selection, target cells are not bound by the selecting reagents.
R&D Systems MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic). This feature protects purified cell populations against magnetic forces which may affect cell viability and interfere with downstream applications. By virtue of their small size (~150 nm diameter), MagCellect Ferrofluids behave like colloidal particles, easily remaining in solution and allowing for efficient diffusion kinetics in the binding reaction.
Purity of cells isolated with MagCellect is typically between 90% and 98%. B cell, T cell, Regulatory T cell, and bone marrow lineage depletion kits are among the many MagCellect isolation kits offered.
|B cells||B cells||B cells|
|Blood dendritic cells||CD3+ T cells||CD3+ T cells|
|Blood myeloid dendritic cells||CD4+ T cells||CD4+ T cells|
|CD3+ T cells||CD4+ T cells (memory)||CD4+ CD25+ Reg T cells|
|CD4+ T cells||CD4+ T cells (naive)|
|CD4+ T cells (memory)||CD8+ T cells|
|CD8+ T cells||CD8+ T cells (naive)|
|CD8+ T cells (memory)||CD4+ CD25+ Reg T cells|
|CD8+ T cells (naive)||Hematopoietic lineage cells|
|CD14+ cells||Mesenchymal stem cells|
|CD4+ CD25+ Reg T cells||NK cells|
||Figure 1. Mouse splenocytes before (A) and after (B) separation of CD3+ T cells using R&D Systems' MagCellect CD3+ T Cell Isolation Kit (Catalog # MAGM201). Histograms reflect viable cells stained with CD3-FITC. Purity of all isolated cells for this experiment was 97.5%.|
||Figure 2. Ficolled human PBMCs before (A) and after (B) separation of B cells using R&D Systems' MagCellect Human B Cell Isolation Kit (Catalog # MAGH103). Histograms reflect viable cells stained with CD19-FITC. Purity of all isolated cells for this experiment was 98%.|