MagCellect™ Cell Selection Kits & Reagents

MagCellect Cell Selection Kits provide a powerful tool for purifying cell populations by negative selection.

View Full Assay Principle

MagCellect is a great choice when a population of pure and “untouched” cells is required. For example, eosinophils, T cells, and other populations often become “activated” upon binding to antibody magnetic beads. Because MagCellect Cell Selection kits use negative selection, target cells are not bound by the selecting reagents.

R&D Systems MagCellect technology is based on the use of ferrofluids or magnetic nanoparticles that have no magnetic memory (superparamagnetic). This feature protects purified cell populations against magnetic forces which may affect cell viability and interfere with downstream applications. By virtue of their small size (~150 nm diameter), MagCellect Ferrofluids behave like colloidal particles, easily remaining in solution and allowing for efficient diffusion kinetics in the binding reaction.

Purity of cells isolated with MagCellect is typically between 90% and 98%. B cell, T cell, Regulatory T cell, and bone marrow lineage depletion kits are among the many MagCellect isolation kits offered.

Features

  • Beads have high binding capacity
  • Small beads do not induce cell damage
  • Cells can be separated to a very high purity within minutes
  • Ferrofluids have no magnetic memory that could damage purified cells
  • Efficient diffusion kinetics in the binding reaction
  • Purity of isolated cells is 90-98%

Kit Contents

  • MagCellect Antibody Cocktail
  • MagCellect Ferrofluid - conjugated to either goat anti-mouse IgG or streptavidin
  • MagCellect 10X Buffer

Other Materials Required

  • MagCellect Cell Selection Magnet (Catalog # MAG997 or equivalent).
Human Mouse Rat
B cells B cells B cells
Blood dendritic cells CD3+ T cells CD3+ T cells
Blood myeloid dendritic cells CD4+ T cells CD4+ T cells
CD3+ T cells CD4+ T cells (memory) CD4+ CD25+ Reg T cells
CD4+ T cells CD4+ T cells (naive)  
CD4+ T cells (memory) CD8+ T cells  
CD8+ T cells CD8+ T cells (naive)  
CD8+ T cells (memory) CD4+ CD25+ Reg T cells  
CD8+ T cells (naive) Hematopoietic lineage cells  
CD14+ cells Mesenchymal stem cells  
CD4+ CD25+ Reg T cells NK cells  
Eosinophils    
NK cells    
A
B
Figure 1. Mouse splenocytes before (A) and after (B) separation of CD3+ T cells using R&D Systems' MagCellect CD3+ T Cell Isolation Kit (Catalog # MAGM201). Histograms reflect viable cells stained with CD3-FITC. Purity of all isolated cells for this experiment was 97.5%.
A
B
Figure 2. Ficolled human PBMCs before (A) and after (B) separation of B cells using R&D Systems' MagCellect Human B Cell Isolation Kit (Catalog # MAGH103). Histograms reflect viable cells stained with CD19-FITC. Purity of all isolated cells for this experiment was 98%.