Kits include two different antibodies for capture and detection of desired cell type
Negative selection of Human B cells from PBMCs or whole blood (Catalog # MAGH103)
Isolation of human CD19+ B cells from PBMCs and whole blood using the MagCellect Human B Cell Isolation kit. Human PBMCs (top) and whole blood (bottom) were prepared as described in the Cell Preparation section of the kit insert. Cells Were stained with Mouse Anti-Human CD19-PE Monoclonal Antibody (Catalog # FAB4867P) and Mouse Anti-Human CD14-APC Monoclonal Antibody (Catalog # FAB3832A), either before (left) or after (right) isolation of B cells.
Positive selection of EGFR+ cells (Catalog # MAGH124)
Isolation of human EGFR+ cells from a cell mixture using the MagCellect Plus Human EGFR+ Cell Isolation Kit. Human PBMCs were prepared as described in the Cell Preparation Section of the kit insert. Cells were stained with Mouse Anti-Human EGFR- AlexaFluor® 647 Monoclonal Antibody (included in the kit) and Mouse Anti-Human CD45-PE Conjugated Monoclonal Antibody (Catalog # FAB1430P) either before (first column “Pre”) or after (middle column “Post”) isolation of EGFR+ cells. The third column shows staining of cells in the negative fraction.
Other Materials Required
MagCellect Cell Selection Magnet (Catalog # MAG997 or equivalent)
Cell Enrichment Columns are also available to purify all T cells and specific T cell subpopulations by high affinity negative selection.