MagCellect™ Cell Selection Kits & Reagents

MagCellect Cell Selection Kits provide a powerful tool for enriching cell populations by positive or negative selection without the need for specialized columns.

Features

  • Small beads have high binding capacity
  • Small beads in ferrofluid have no magnetic memory and do not induce cell damage
  • Cells can be separated to a very high purity in minutes

 

Browse By Cell Type

B CellsCD24-CD44+ Breast cancer stem cell
CD3+ T cellsEpCam+ cells
CD4+ T cellsCD44+ cells
CD4+ T cells (naïve)E-Cadherin+ cells
CD4+ T cells (memory)EGFR+ cells
CD4+ CD25+ T RegsCD45+ cells
CD8+ T cellsHematopoietic Cell Lineage Depletion
CD14+ monocytes 
NK cells 
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MagCellect™ Negative Selection Kits

  • Enrichment of desired cell populations
  • Isolated cells free from bound antibody for downstream applications 

Step 1

MagCellect Negative Selection Kit: biotinylated antibody and streptavidin-conjugated magnetic beads are added to desired cell population.

Step 2

MagCellect Negative Selection Kit: undesired cells are separated out from desired cell population.

Example Data:

Negative selection of Human B cells from PBMCs or whole blood.

Isolation of human CD19+ B cells from PBMCs and whole blood using the MagCellect Human B Cell Isolation Kit. Human PBMCs (top) and whole blood (bottom) were prepared as described in the Cell Preparation section of the insert for the MagCellect Human B Cell Isolation Kit (Catalog # MAGH103). Cells were stained with a PE-conjugated Mouse Anti-Human CD19 Monoclonal Antibody (Catalog # FAB4867P) and an APC-conjugated Mouse Anti-Human CD14 Monoclonal Antibody (Catalog # FAB3832A), either before (left) or after (right) isolation of B cells.

MagCellect™ Plus Positive Selection Kits

  • Selection of rare cell populations from heterogenous sample
  • Very high purity
  • Kits include fluorescently conjugated antibody for post selection purity assessment by flow cytometry 

Step 1

MagCellect Positive Isolation Kit: biotinylated antibody and streptavidin-conjugated magnetic beads are added to the cell population.

Step 2

MagCellect™ Plus Positive Selection Kits: undesired cells are separated from desired cell population.

Example Data:

Positive selection of EGFR+ cell.

Isolation of human EGFR+ cells from a cell mixture using the MagCellect Plus Human EGFR+ Cell Isolation Kit. Human PBMCs were prepared as described in the Cell Preparation Section of the insert for the MagCellect Plus Human EGFR+ Cell Isolation Kit (Catalog # MAGH124). Cells were stained with an AlexaFluor® 647-conjugated Mouse Anti-Human EGFR Monoclonal Antibody (included in the kit) and a PE-conjugated Mouse Anti-Human CD45 Monoclonal Antibody (Catalog # FAB1430P) either before (first column “Pre”) or after (middle column “Post”) isolation of EGFR+ cells. The third column shows staining of cells in the negative fraction.

 

MagCellect Cell Selection Magnet image

Other Materials Required

MagCellect Cell Selection Magnet (Catalog # MAG997 or equivalent)

Related Information