MBP Antibody (12) - BSA Free

Novus Biologicals | Catalog # NB600-717

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Validated:

Bovine

Cited:

Human, Mouse, Rat, Bovine, Canine, Feline, Guinea Pig, Primate

Applications

Validated:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, ELISA, Immunocytochemistry/ Immunofluorescence, Radioimmunodiffusion, Radioimmunoassay

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Immunohistochemistry-Frozen, Western Blot, Immunocytochemistry/ Immunofluorescence, IF/IHC

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 12

Format

BSA Free
View all MBP Primary Antibodies »

Product Specifications

Immunogen

Bovine MBP

Epitope

Amino acids 82-87.

Reactivity Notes

Predicted cross-reactivities: Mouse, Rabbit, Porcine, Mammals, Human, Guinea Pig, Chicken, Rat, Sheep

Localization

Cytoplasmic side of myelin

Marker

Oligodendrocyte Marker, Myelin Marker

Specificity

NB600-717 reacts with myelin basic protein from a wide range of species. The antibody reacts weakly with peptides ending in the Phe 91 where the 91-92 Phe-Phe bond is broken. Synthetic peptide 82-99 reacts very well, as does intact MBP. Further epitope analysis indicates binding to a region defined by amino acids 82-87 (DENPVV). Clone 12 has been reported as being suitable for use in Western blotting.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for MBP Antibody (12) - BSA Free

Western Blot: MBP Antibody (12) [NB600-717]

Western Blot: MBP Antibody (12) [NB600-717]

Western Blot: MBP Antibody (12) [NB600-717] - Mouse Brain Tissue lysate probed with Rat anti MBP.
Immunocytochemistry/ Immunofluorescence: MBP Antibody (12) [NB600-717]

Immunocytochemistry/ Immunofluorescence: MBP Antibody (12) [NB600-717]

MBP-Antibody-12-Immunocytochemistry-Immunofluorescence-NB600-717-img0005.jpg
Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717]

Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717]

Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse brain corpus callosum. Image from verified customer review.
Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717]

Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717]

Immunohistochemistry-Frozen: MBP Antibody (12) [NB600-717] - Staining of MBP in mouse spinal cord ventral white matter. Image from verified customer review.
MBP Antibody (12)

Immunocytochemistry/ Immunofluorescence: MBP Antibody (12) [NB600-717] -

Immunocytochemistry/ Immunofluorescence: MBP Antibody (12) [NB600-717] - Microglia processes appear to preferentially contact TBI-induced proximal axonal swellings. Representative 3D reconstructions of MBP+ myelinated axons (red) or APP+ axonal swellings (green) & Iba-1+ microglia (white) in sham-injured (a) or central fluid percussion injured (b) thalami. c Bar graph depicting the average number of Iba-1+ microglial processes contacting either MBP+ myelinated fibers in the sham animals or APP+ axonal swellings in injured animals. Graph depicts the mean ± standard error of the mean. *p < 0.05. Scale bar: 5 μm Image collected & cropped by CiteAb from the following publication (https://jneuroinflammation.biomedcentral.com/articles/10.1186/s12974-01…), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MBP Antibody (12) - BSA Free

Western Blot: MBP Antibody (12) - BSA Free [NB600-717] -

Western blots based on protein quantification with the PDB assay. (A,B) Ponceau S-stained dot blot. Undiluted BSA standards and BSA standards diluted 1:1 in 2x SDS lysis buffer were spotted in duplicate onto a membrane (fixed concentration, variable volumes). A quantity of 1 uL of sciatic nerve (SN) and brain samples lysed in 2x SDS LB or RIPA buffer was also applied onto the same membrane for quantification with the PDB assay. (C) The nerve and brain lysates containing 50, 25 or 15 ug of total proteins (based on the PDB assay) were loaded for SDS-PAGE and Western blot. After protein transfer to a nitrocellulose membrane, the membrane was also stained with Ponceau S. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/35049578), licensed under a CC-BY license. Not internally tested by Novus Biologicals.
MBP Antibody (12) - BSA Free

Western Blot: MBP Antibody (12) - BSA Free [NB600-717] -

Liraglutide enhances the normal physiological function of SCs via insulin-Akt signaling. (a) Western blot analysis of Ser473-phosphorylated Akt confirmed that Liraglutide and insulin reverse the glucolipotoxicity-induced insulin signaling blockade. (b) MTT assays showed that the protective effect of Liraglutide and insulin were inhibited by co-treatment with 20 μM LY294002. (c) mRNA levels of neurotrophic factors, including CNTF, NGF, NT-3, and BDNF, were measured by qPCR. Liraglutide and insulin significantly elevated the mRNA levels of neurotrophic factors suppressed by glucolipotoxicity. However, LY294002 counteracted the effects of Liraglutide and insulin. (d) Western blots demonstrated that Liraglutide and insulin show efficacy in improving SC synthesis of essential myelin components and decrease the expression of the demyelination marker RAGE. Similarly, LY294002 blocked the effects of Liraglutide and insulin in promoting myelination in RSC96 SCs. All values are presented as the mean +/- SEM. Significant difference was determined using multiple comparisons of Dunnett’s posthoc test for * p < 0.05 and ** p < 0.01. N.S., not significant. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36291547), licensed under a CC-BY license. Not internally tested by Novus Biologicals.

Applications for MBP Antibody (12) - BSA Free

Application
Recommended Usage

ELISA

1:100-1:2000

Immunocytochemistry/ Immunofluorescence

1:10-1:500

Immunohistochemistry

1:10-1:500

Immunohistochemistry-Frozen

1:10-1:500

Immunohistochemistry-Paraffin

1:10-1:500

Western Blot

1:100-1:2000

Reviewed Applications

Read 3 reviews rated 4.7 using NB600-717 in the following applications:

Formulation, Preparation, and Storage

Purification

Tissue culture supernatant

Formulation

0.1 M Tris

Format

BSA Free

Preservative

0.1% Sodium Azide

Concentration

This product is unpurified. The exact concentration of antibody is not quantifiable.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MBP

Myelin Basic Protein (MBP) functions in the nervous system in myelination and is expressed in oligodendrocytes following differentiation. There are numerous isoforms generated by differential splicing events and post-translational modifications that have specialized functions. These functions include participation in signaling pathways prior to myelination, myelination or the re-myelination process following neural injury.

Long Name

Myelin Basic Protein

Alternate Names

Hmbpr, mld, shi

Entrez Gene IDs

4155 (Human); 17196 (Mouse); 24547 (Rat); 618684 (Bovine)

Gene Symbol

MBP

UniProt

P02687 (Bovine)

Additional MBP Products

Product Documents for MBP Antibody (12) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Product Specific Notices for MBP Antibody (12) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Related Research Areas

Glial Lineage Markers

Citations for MBP Antibody (12) - BSA Free

Customer Reviews for MBP Antibody (12) - BSA Free (3)

4.7 out of 5
3 Customer Ratings
5 Stars
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4 Stars
33%
3 Stars
0%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 3 of 3 reviews Showing All
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  • MBP Antibody (12)
    Name: Anonymous
    Application: Western Blot
    Sample Tested: brain and spinal cord
    Species: Mouse
    Verified Customer | Posted 04/13/2019
    MBP Western blot in adult mice brain tissue
    MBP Antibody (12) - BSA Free NB600-717
  • MBP Antibody (12)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: brain and spinal cord
    Species: Mouse
    Verified Customer | Posted 04/13/2019
    MBP stain in brain corpus callosum
    MBP Antibody (12) - BSA Free NB600-717
  • MBP Antibody (12)
    Name: Anonymous
    Application: Immunohistochemistry-Frozen
    Sample Tested: brain and spinal cord
    Species: Feline
    Verified Customer | Posted 12/08/2017
    NB600-717 was incubated at 1:100 dilution for overnight at 4C degree and Alexa488 conjugated antibody was used. Image was captured with epifluorescent microscope.
    MBP Antibody (12) - BSA Free NB600-717

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Protocols

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