MEF2C Antibody (CL0368) - BSA Free

Novus Biologicals | Catalog # NBP2-52925

Novus Biologicals

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Knockdown Validated

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0368

Format

BSA Free
View all MEF2C Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q06413, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MEF2C Antibody (CL0368) - BSA Free

Western Blot: MEF2C Antibody (CL0368) [NBP2-52925]

Western Blot: MEF2C Antibody (CL0368) [NBP2-52925]

Western Blot: MEF2C Antibody (CL0368) [NBP2-52925] - Analysis in WM115 cells transfected with control siRNA, target specific siRNA probe #1 and #2, using Anti-MEF2C antibody. Remaining relative intensity is presented. Loading control: Anti-GAPDH.
Immunocytochemistry/ Immunofluorescence: MEF2C Antibody (CL0368) [NBP2-52925]

Immunocytochemistry/ Immunofluorescence: MEF2C Antibody (CL0368) [NBP2-52925]

Immunocytochemistry/Immunofluorescence: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of RH-30 cells using the Anti-MEF2C monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925]

Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925]

Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human tonsil shows strong nuclear immunoreactivity in a subset of lymphoid cells.
Western Blot: MEF2C Antibody (CL0368) [NBP2-52925]

Western Blot: MEF2C Antibody (CL0368) [NBP2-52925]

Western Blot: MEF2C Antibody (CL0368) [NBP2-52925] - Lane 1: Marker [kDa], Lane 2: Negative control (vector only transfected HEK293T lysate), Lane 3: MEF2C Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925]

Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925]

Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human cerebellum shows strong nuclear positivity in Purkinje cells.
Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925]

Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925]

Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human cerebral cortex shows strong immunoreactivity in neuronal cells.
Immunohistochemistry: MEF2C Antibody (CL0368) [NBP2-52925]

Immunohistochemistry: MEF2C Antibody (CL0368) [NBP2-52925]

Immunohistochemistry: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human duodenum shows strong nuclear immunoreactivity in a subset of lymphoid cells.
Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925]

Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925]

Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human pancreas shows absense of staining (negative control).
Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925]

Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925]

Immunohistochemistry-Paraffin: MEF2C Antibody (CL0368) [NBP2-52925] - Staining of human skeletal muscle shows moderate nuclear positivity in muscle fibers.

Applications for MEF2C Antibody (CL0368) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF,Fixation/Permeabilization: PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MEF2C

Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. May also be involved in neurogenesis and in the development of cortical architecture. Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2

Long Name

Myocyte-specific Enhancer Factor 2C

Alternate Names

DEL5q14.3

Gene Symbol

MEF2C

Additional MEF2C Products

Product Documents for MEF2C Antibody (CL0368) - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for MEF2C Antibody (CL0368) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for MEF2C Antibody (CL0368) - BSA Free

There are currently no reviews for this product. Be the first to review MEF2C Antibody (CL0368) - BSA Free and earn rewards!

Have you used MEF2C Antibody (CL0368) - BSA Free?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies