MEF2C Antibody (CL0369) - BSA Free

Novus Biologicals | Catalog # NBP2-30671

Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # CL0369

Format

BSA Free
View all MEF2C Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed using a recombinant protein derived from Q06413, with the exact immunogen sequence remaining proprietary.

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for MEF2C Antibody (CL0369) - BSA Free

Western Blot: MEF2C Antibody (CL0369) [NBP2-30671]

Western Blot: MEF2C Antibody (CL0369) [NBP2-30671]

Western Blot: MEF2C Antibody (CL0369) [NBP2-30671] - Lane 1: Marker [kDa] 250, 130, 95, 72, 55, 36, 28, 17, 10. Lane 2: Negative control (vector only transfected HEK293T lysate). Lane 3: Over-expression lysate (Co-expressed with a C-terminal myc-DDK tag (3.1 kDa) in mammalian HEK293T cells).
Immunocytochemistry/ Immunofluorescence: MEF2C Antibody (CL0369) [NBP2-30671]

Immunocytochemistry/ Immunofluorescence: MEF2C Antibody (CL0369) [NBP2-30671]

Immunocytochemistry/Immunofluorescence: MEF2C Antibody (CL0369) [NBP2-30671] - Staining of RH-30 cells using the Anti-MEF2C monoclonal antibody, showing specific staining in the nucleoplasm in green. Microtubule- and nuclear probes are visualized in red and blue, respectively (where available).
Immunohistochemistry: MEF2C Antibody (CL0369) [NBP2-30671]

Immunohistochemistry: MEF2C Antibody (CL0369) [NBP2-30671]

Immunohistochemistry: MEF2C Antibody (CL0369) [NBP2-30671] - Staining of human pancreas shows absence of staining in the exocrine glandular cells (negative control).
Immunohistochemistry: MEF2C Antibody (CL0369) [NBP2-30671]

Immunohistochemistry: MEF2C Antibody (CL0369) [NBP2-30671]

Immunohistochemistry: MEF2C Antibody (CL0369) [NBP2-30671] - Staining of human cerebral cortex shows nuclear immunoreactivity in a subset of neurons.
Immunohistochemistry: MEF2C Antibody (CL0369) [NBP2-30671]

Immunohistochemistry: MEF2C Antibody (CL0369) [NBP2-30671]

Immunohistochemistry: MEF2C Antibody (CL0369) [NBP2-30671] - Staining of human skeletal muscle shows distinct nuclear positivity in the muscle fibers.
Immunohistochemistry: MEF2C Antibody (CL0369) [NBP2-30671]

Immunohistochemistry: MEF2C Antibody (CL0369) [NBP2-30671]

Immunohistochemistry: MEF2C Antibody (CL0369) [NBP2-30671] - Staining of human cerebellum shows nuclear immunoreactivity in Purkinje cells, as well as in neuronal cells in the granular and molecular layers.
Immunohistochemistry: MEF2C Antibody (CL0369) [NBP2-30671]

Immunohistochemistry: MEF2C Antibody (CL0369) [NBP2-30671]

Immunohistochemistry: MEF2C Antibody (CL0369) [NBP2-30671] - Staining of human tonsil shows strong nuclear immunoreactivity in the germinal center cells.

Applications for MEF2C Antibody (CL0369) - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

2-10 ug/ml

Immunohistochemistry

1:500 - 1:1000

Immunohistochemistry-Paraffin

1:500 - 1:1000

Western Blot

1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For ICC/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

1 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MEF2C

Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. May also be involved in neurogenesis and in the development of cortical architecture. Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2

Long Name

Myocyte-specific Enhancer Factor 2C

Alternate Names

DEL5q14.3

Gene Symbol

MEF2C

UniProt

Q06413

Additional MEF2C Products

Product Documents for MEF2C Antibody (CL0369) - BSA Free

Certificate of Analysis

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Product Specific Notices for MEF2C Antibody (CL0369) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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