MEF2C Antibody (CL0369) - Azide and BSA Free
Novus Biologicals | Catalog # NBP3-44010
Key Product Details
Species Reactivity
Human
Applications
Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # CL0369
Format
Azide and BSA Free
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Product Specifications
Immunogen
This antibody was generated using a recombinant protein sequence of Q06413, with the exact immunogen sequence remaining proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for MEF2C Antibody (CL0369) - Azide and BSA Free
![MEF2C Antibody (CL0369) - Azide and BSA Free Western Blot: MEF2C Antibody [NBP3-44010]](https://resources.rndsystems.com/images/products/nbp3-44010_mouse-mef2c-mab-cl0369-azide-and-bsa-free-28220251211183.jpg "Western Blot: MEF2C Antibody [NBP3-44010]")
Western Blot: MEF2C Antibody [NBP3-44010]
Lane 1: Marker [kDa]Lane 2: Negative control (vector only transfected HEK293T lysate)
Lane 3: MEF2C Over-expression Lysate (Co-expressed with a C-terminal myc-DDK tag (~3.1 kDa) in mammalian HEK293T cells)
![MEF2C Antibody (CL0369) - Azide and BSA Free Immunohistochemistry-Paraffin: MEF2C Antibody [NBP3-44010]](https://resources.rndsystems.com/images/products/nbp3-44010_mouse-mef2c-mab-cl0369-azide-and-bsa-free-282202512195435.jpg "Immunohistochemistry-Paraffin: MEF2C Antibody [NBP3-44010]")
Immunohistochemistry-Paraffin: MEF2C Antibody [NBP3-44010]
Staining of human cerebellum shows nuclear immunoreactivity in Purkinje cells, as well as in neuronal cells in the granular and molecular layers.![MEF2C Antibody (CL0369) - Azide and BSA Free Immunohistochemistry-Paraffin: MEF2C Antibody [NBP3-44010]](https://resources.rndsystems.com/images/products/nbp3-44010_mouse-mef2c-mab-cl0369-azide-and-bsa-free-28220251235131.jpg "Immunohistochemistry-Paraffin: MEF2C Antibody [NBP3-44010]")
Immunohistochemistry-Paraffin: MEF2C Antibody [NBP3-44010]
Staining of human cerebral cortex shows nuclear immunoreactivity in a subset of neurons.![MEF2C Antibody (CL0369) - Azide and BSA Free Immunohistochemistry-Paraffin: MEF2C Antibody [NBP3-44010]](https://resources.rndsystems.com/images/products/nbp3-44010_mouse-mef2c-mab-cl0369-azide-and-bsa-free-282202513121837.jpg "Immunohistochemistry-Paraffin: MEF2C Antibody [NBP3-44010]")
Immunohistochemistry-Paraffin: MEF2C Antibody [NBP3-44010]
Staining of human pancreas shows absence of staining in the exocrine glandular cells (negative control).![MEF2C Antibody (CL0369) - Azide and BSA Free Immunohistochemistry-Paraffin: MEF2C Antibody [NBP3-44010]](https://resources.rndsystems.com/images/products/nbp3-44010_mouse-mef2c-mab-cl0369-azide-and-bsa-free-2822025139193.jpg "Immunohistochemistry-Paraffin: MEF2C Antibody [NBP3-44010]")
Immunohistochemistry-Paraffin: MEF2C Antibody [NBP3-44010]
Staining of human skeletal muscle shows distinct nuclear positivity in the muscle fibers.![MEF2C Antibody (CL0369) - Azide and BSA Free Immunohistochemistry-Paraffin: MEF2C Antibody [NBP3-44010]](https://resources.rndsystems.com/images/products/nbp3-44010_mouse-mef2c-mab-cl0369-azide-and-bsa-free-28220251339190.jpg "Immunohistochemistry-Paraffin: MEF2C Antibody [NBP3-44010]")
Immunohistochemistry-Paraffin: MEF2C Antibody [NBP3-44010]
Staining of human tonsil shows strong nuclear immunoreactivity in the germinal center cells.![MEF2C Antibody (CL0369) - Azide and BSA Free Immunocytochemistry/ Immunofluorescence: MEF2C Antibody [NBP3-44010]](https://resources.rndsystems.com/images/products/nbp3-44010_mouse-mef2c-mab-cl0369-azide-and-bsa-free-282202513245477.jpg "Immunocytochemistry/ Immunofluorescence: MEF2C Antibody [NBP3-44010]")
Immunocytochemistry/ Immunofluorescence: MEF2C Antibody [NBP3-44010]
Staining of RH-30 cells using the Anti-MEF2C monoclonal antibody).Applications for MEF2C Antibody (CL0369) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
2-10 ug/ml
Immunohistochemistry-Paraffin
1:500 - 1:1000
Western Blot
1 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. For Immunocytochemistry/ Immunofluorescence/IF, PFA/Triton X-100 is recommended for fixation/permeabilization.
Formulation, Preparation, and Storage
Purification
Protein A purified
Reconstitution
Centrifuge the vial of lyophilized antibody at 12,000 x g for 20 seconds. Reconstitute by adding sterile, distilled water to achieve a final antibody concentration of 1mg/ml.
Formulation
Lyophilized from a 0.2 um filtered solution in PBS with Trehalose
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
LYOPH mg/ml
Shipping
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.
Calculators
Background: MEF2C
Long Name
Myocyte-specific Enhancer Factor 2C
Alternate Names
DEL5q14.3
Gene Symbol
MEF2C
Additional MEF2C Products
Product Documents for MEF2C Antibody (CL0369) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for MEF2C Antibody (CL0369) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Antigen Retrieval Protocol (PIER)
- Antigen Retrieval for Frozen Sections Protocol
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- Chromogenic IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Chromogenic Immunohistochemistry Staining of Frozen Tissue
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- Fluorescent IHC Staining of Frozen Tissue Protocol
- Graphic Protocol for Heat-induced Epitope Retrieval
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Graphic Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Graphic Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- IHC Sample Preparation (Frozen sections vs Paraffin)
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunofluorescent IHC Staining of Formalin-Fixed Paraffin-Embedded (FFPE) Tissue Protocol
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunohistochemistry Frozen Troubleshooting
- Immunohistochemistry Paraffin Troubleshooting
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for Heat-Induced Epitope Retrieval (HIER)
- Protocol for Making a 4% Formaldehyde Solution in PBS
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation & Fixation of Cells on Coverslips
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Frozen Tissue Sections - Graphic
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation and Chromogenic IHC Staining of Paraffin-embedded Tissue Sections - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation and Fluorescent IHC Staining of Frozen Tissue Sections
- Protocol for the Preparation and Fluorescent IHC Staining of Paraffin-embedded Tissue Sections
- Protocol for the Preparation of Gelatin-coated Slides for Histological Tissue Sections
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Immunohistochemistry
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
