MEF2C Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-89468

Novus Biologicals
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Key Product Details

Validated by

Orthogonal Validation

Species Reactivity

Validated:

Human

Predicted:

Mouse (97%), Rat (99%). Backed by our 100% Guarantee.

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation-exo-Seq

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all MEF2C Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: PPNFEMPVSIPVSSHNSLVYSNPVSSLGNPNLLPLAHPSLQRNSMSPGVTHRPPSAGNTGGLMGGDLTSGAGTSAGNGYGNPRNSPGLLVSPGNLNKNMQAKSPPPMNLGMNNRKPDLRVLIPPGSKNTMPSVNQRINN

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for MEF2C Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: MEF2C Antibody [NBP1-89468]

Immunocytochemistry/ Immunofluorescence: MEF2C Antibody [NBP1-89468]

Immunocytochemistry/Immunofluorescence: MEF2C Antibody [NBP1-89468] - Staining of human cell line RH-30 shows localization to nucleoplasm & vesicles. Antibody staining is shown in green.
MEF2C Antibody - BSA Free Immunohistochemistry-Paraffin: MEF2C Antibody - BSA Free [NBP1-89468]

Immunohistochemistry-Paraffin: MEF2C Antibody - BSA Free [NBP1-89468]

Analysis in human cerebral cortex and liver tissues using NBP1-89468 antibody. Corresponding MEF2C RNA-seq data are presented for the same tissues.
MEF2C Antibody - BSA Free Immunohistochemistry-Paraffin: MEF2C Antibody - BSA Free [NBP1-89468]

Immunohistochemistry-Paraffin: MEF2C Antibody - BSA Free [NBP1-89468]

Staining of human liver shows no positivity in hepatocytes as expected.
MEF2C Antibody - BSA Free Immunohistochemistry-Paraffin: MEF2C Antibody - BSA Free [NBP1-89468]

Immunohistochemistry-Paraffin: MEF2C Antibody - BSA Free [NBP1-89468]

Staining of human cerebral cortex shows strong nuclear positivity in neurons.
MEF2C Antibody - BSA Free Immunohistochemistry-Paraffin: MEF2C Antibody - BSA Free [NBP1-89468]

Immunohistochemistry-Paraffin: MEF2C Antibody - BSA Free [NBP1-89468]

Staining of human skeletal muscle shows moderate nuclear positivity in myocytes.
MEF2C Antibody - BSA Free Immunohistochemistry-Paraffin: MEF2C Antibody - BSA Free [NBP1-89468]

Immunohistochemistry-Paraffin: MEF2C Antibody - BSA Free [NBP1-89468]

Staining of human tonsil shows moderate nuclear positivity in germinal center cells.
MEF2C Antibody - BSA Free Western Blot: MEF2C Antibody - BSA Free [NBP1-89468]

Western Blot: MEF2C Antibody - BSA Free [NBP1-89468]

Analysis in human cerebral cortex and liver tissues using NBP1-89468 antibody. Corresponding MEF2C RNA-seq data are presented for the same tissues.
MEF2C Antibody - BSA Free Chromatin Immunoprecipitation-exo-Seq: MEF2C Antibody - BSA Free [NBP1-89468]

Chromatin Immunoprecipitation-exo-Seq: MEF2C Antibody - BSA Free [NBP1-89468]

ChIP-Exo-Seq composite graph for Anti-MEF2C (NBP1-89468) tested in K562 cells. Strand-specific reads (blue: forward, red: reverse) and IgG controls (black: forward, grey: reverse) are plotted against the distance from a composite set of reference binding sites. The antibody exhibits robust target enrichment compared to a non-specific IgG control and precisely reveals its structural organization around the binding site. Data generated by Prof. B. F. Pugh´s Lab at Cornell University.

Applications for MEF2C Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation-exo-Seq

1-10ug per reaction

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Immunohistochemistry

1:50 - 1:200

Immunohistochemistry-Paraffin

1:50 - 1:200

Western Blot

0.04-0.4 ug/ml
Application Notes
For IHC-Paraffin, HIER pH 6 retrieval is recommended. ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: MEF2C

Transcription activator which binds specifically to the MEF2 element present in the regulatory regions of many muscle-specific genes. Controls cardiac morphogenesis and myogenesis, and is also involved in vascular development. May also be involved in neurogenesis and in the development of cortical architecture. Isoform 3 and isoform 4, which lack the repressor domain, are more active than isoform 1 and isoform 2

Long Name

Myocyte-specific Enhancer Factor 2C

Alternate Names

DEL5q14.3

Gene Symbol

MEF2C

Additional MEF2C Products

Product Documents for MEF2C Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for MEF2C Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for MEF2C Antibody - BSA Free

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Protocols

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FAQs

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