MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free

Name: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free
Brand: Novus Biologicals
SKU: NBP2-72710
Price: 559 USD
Availability: InStock

Novus Biologicals | Catalog # NBP2-72710

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Product Specifications
Scientific Data
Applications
Flow Cytometry
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation, CyTOF-ready

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG_2B Clone # OTI8A8

Format

Azide and BSA Free

View all MKK4 Primary Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human MAP2K4 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG_2B

Theoretical MW

44.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free

Western Blot: MKK4/MEK4 Antibody (OTI8A8)Azide and BSA Free [NBP2-72710]

Western Blot: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710] - Analysis of extracts (35ug) from 9 different cell lines by using anti-MEK4 monoclonal antibody.

Immunocytochemistry/ Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710]

Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710] - Staining of HepG2 cells using anti-MEK4 mouse monoclonal antibody.

Immunohistochemistry: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710]

Immunohistochemistry: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710] - Staining of paraffin-embedded Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.

Flow Cytometry: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710]

Flow Cytometry: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEK4.

Immunohistochemistry: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710]

Immunohistochemistry: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MEK4 mouse monoclonal antibody.

Immunohistochemistry: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710]

Immunohistochemistry: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.

Immunohistochemistry: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710]

Immunohistochemistry: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710] - Staining of paraffin-embedded Human pancreas tissue using anti-MEK4 mouse monoclonal antibody.

Flow Cytometry: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710]

Flow Cytometry: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostaining by anti-MEK4 antibody, and then analyzed by flow cytometry.

Immunoprecipitation: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710]

Immunoprecipitation: MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free [NBP2-72710] - (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of DDK tagged MAP2K4 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAP2K4 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

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Applications for MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free

Application

Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-100

Immunohistochemistry

1:150

Immunoprecipitation

2ug/500ul

Western Blot

1:500-1000

Flow Cytometry Panel Builder

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Save time and reduce costly mistakes by quickly finding compatible reagents using the Panel Builder Tool.

Advanced Features

Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
Spillover Popups - Visualize the spectra of individual fluorochromes
Antigen Density Selector - Match fluorochrome brightness with antigen density

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Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Reconstitution

we recommend adding 100uL distilled water to a final antibody concentration of about 1 mg/mL. To use this carrier-free antibody for conjugation experiment, we strongly recommend performing another round of desalting process.

Formulation

Lyophilized from PBS (pH 7.3) with 8% Trehalose

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

LYOPH mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: MKK4

MKK4, also known as JNKK1 and SEK1, is a dual specificity kinase that belongs to the Ser/Thr protein kinase family. MKK4 is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. MKK4 phosphorylates and activates p38 MAP kinase and JNK but not ERK 1/2 (1-2). JNK is activated by dual phosphorylation at Thr and Tyr by MKK4 and MKK7; although MKK4 will preferentially phosphorylate Tyr (3). MKK4 is also known to be a tumor suppressor, as mutations inactivating the MKK4 gene have been found in 5% of a wide variety of tumor types (4).

Long Name

Mitogen-activated Protein Kinase Kinase 4

Alternate Names

JNKK1, MAP2K4, MAPKK4, MEK4, PRKMK4, SERK1

Gene Symbol

MAP2K4

Additional MKK4 Products

Product Documents for MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free

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Certificate of Analysis

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Product Specific Notices for MKK4/MEK4 Antibody (OTI8A8) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.