MKK4/MEK4 Antibody (OTI8A8)

Novus Biologicals | Catalog # NBP1-47839

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat, Canine

Applications

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Flow Cytometry, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # OTI8A8
View all MKK4 Primary Antibodies »

Product Specifications

Immunogen

Full-length protein expressed in 293T cell transfected with human MAP2K4 expression vector

Reactivity Notes

Please note that this antibody is reactive to Mouse and derived from the same host, Mouse. Mouse-On-Mouse blocking reagent may be needed for IHC and ICC experiments to reduce high background signal. You can find these reagents under catalog numbers PK-2200-NB and MP-2400-NB. Please contact Technical Support if you have any questions.

Specificity

This antibody is specific for Homo sapiens mitogen-activated protein kinase kinase 4 (MAP2K4).

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Theoretical MW

44.3 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for MKK4/MEK4 Antibody (OTI8A8)

Western Blot: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Western Blot: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Western Blot: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Analysis of extracts (35ug) from 9 different cell lines by using anti-MEK4 monoclonal antibody.
Immunocytochemistry/ Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Immunocytochemistry/ Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of COS7 cells transiently transfected by pCMV6-ENTRY MEK4.
Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded mouse spleen tissue using MKK4/MEK4 antibody (OTI8A8). Image from verified customer review.
Flow Cytometry: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Flow Cytometry: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Flow Cytometry: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - HEK293T cells transfected with either overexpression plasmid (Red) or empty vector control plasmid (Blue) were immunostained by anti-MEK4 antibody, and then analyzed by flow cytometry.
Immunocytochemistry/ Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Immunocytochemistry/ Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Immunocytochemistry/Immunofluorescence: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of HepG2 cells using anti-MEK4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Carcinoma of Human kidney tissue using anti-MEK4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Carcinoma of Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Human pancreas tissue using anti-MEK4 mouse monoclonal antibody.
Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Immunohistochemistry-Paraffin: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - Staining of paraffin-embedded Human thyroid tissue using anti-MEK4 mouse monoclonal antibody.
Immunoprecipitation: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Immunoprecipitation: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839]

Immunoprecipitation: MKK4/MEK4 Antibody (OTI8A8) [NBP1-47839] - (Negative control: IP without adding anti-MAP2K4 antibody.). For each experiment, 500ul of DDK tagged MAP2K4 overexpression lysates (at 1:5 dilution with HEK293T lysate), 2ug of anti-MAP2K4 antibody and 20ul (0.1mg) of goat anti-mouse conjugated magnetic beads were mixed and incubated overnight. After extensive wash to remove any non-specific binding, the immuno-precipitated products were analyzed with rabbit anti-DDK polyclonal antibody.

Applications for MKK4/MEK4 Antibody (OTI8A8)

Application
Recommended Usage

Flow Cytometry

1:100

Immunocytochemistry/ Immunofluorescence

1:50-100

Immunohistochemistry

1:150

Immunohistochemistry-Paraffin

1:150

Immunoprecipitation

2ug/500ul

Western Blot

1:500-1000

Reviewed Applications

Read 1 review rated 5 using NBP1-47839 in the following applications:

Flow Cytometry Panel Builder

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Advanced Features

  • Spectra Viewer - Custom analysis of spectra from multiple fluorochromes
  • Spillover Popups - Visualize the spectra of individual fluorochromes
  • Antigen Density Selector - Match fluorochrome brightness with antigen density
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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

PBS (pH 7.3), 1.0% BSA and 50% Glycerol

Preservative

0.02% Sodium Azide

Concentration

2.36 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: MKK4

This gene encodes a dual specificity protein kinase that belongs to the Ser/Thr protein kinase family. This kinase is a direct activator of MAP kinases in response to various environmental stresses or mitogenic stimuli. It has been shown to activate MAPK8/JNK1, MAPK9/JNK2, and MAPK14/p38, but not MAPK1/ERK2 or MAPK3/ERK3. This kinase is phosphorylated, and thus activated by MAP3K1/MEKK. The knockout studies in mice suggested the roles of this kinase in mediating survival signal in T cell development, as well as in the organogenesis of liver

Long Name

Mitogen-activated Protein Kinase Kinase 4

Alternate Names

JNKK1, MAP2K4, MAPKK4, MEK4, PRKMK4, SERK1

Entrez Gene IDs

6416 (Human)

Gene Symbol

MAP2K4

Additional MKK4 Products

Product Documents for MKK4/MEK4 Antibody (OTI8A8)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for MKK4/MEK4 Antibody (OTI8A8)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Customer Reviews for MKK4/MEK4 Antibody (OTI8A8) (1)

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Customer Images

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  • MKK4/MEK4 Antibody (OTI8A8)
    Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: Spleen tissue
    Species: Mouse
    Verified Customer | Posted 12/21/2021
    Mouse spleen
    MKK4/MEK4 Antibody (OTI8A8) NBP1-47839

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Protocols

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