Mouse AHR Antibody

Catalog # Availability Size / Price Qty
Detection of Mouse AHR by Western Blot.
3 Images
Product Details
Citations (6)
Supplemental Products
Reviews (1)

Mouse AHR Antibody Summary

Species Reactivity
Detects mouse AHR in Western blots.
Polyclonal Sheep IgG
Antigen Affinity-purified
E. coli-derived recombinant mouse AHR
Asn706-Ser805 (Thr758Ala)
Accession # P30561
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.


Recommended Concentration
Western Blot
1 µg/mL
See below
5-15 µg/mL
See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot Detection of Mouse AHR antibody by Western Blot. View Larger

Detection of Mouse AHR by Western Blot. Western blot shows lysates of C2C12 mouse myoblast cell line, mouse lung tissue, Hepa 1-6 mouse hepatoma cell line, Neuro-2A mouse neuroblastoma cell line (negative control), NIH-3T3 mouse embryonic fibroblast cell line (negative control), and mouse cerebellum tissue (negative control). PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6697) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for AHR at approximately 110 kDa (as indicated). GAPDH(Catalog # AF5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry AHR antibody in C2C12 Mouse Cell Line by Immunocytochemistry (ICC). View Larger

AHR in C2C12 Mouse Cell Line. AHR was detected in immersion fixed C2C12 mouse myoblast cell line using Sheep Anti-Mouse AHR Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6697) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red, upper panel; Catalog # NL010) and counterstained with DAPI (blue, lower panel). Specific staining was localized to nuclei and cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Western Blot Detection of Mouse AHR by Western Blot View Larger

Detection of Mouse AHR by Western Blot HIF1 alpha and AhR mediate butyrate induction of IL-22 in CD4+ T cells.a WT CD4+ T cells were activated with anti-CD3/CD28 mAbs under Th1 conditions ± butyrate (0.5 mM) for 2 days (n = 3 biologically independent samples per group). RNA sequencing was performed. Hif1 alpha, Ahr, and Prdm1 were shown in heatmap. b–f CD4+ T cells were activated with anti-CD3/CD28 mAbs ± butyrate (0.5 mM) under Th1 conditions (n = 3/group). Hif1a (b) and Ahr (c) were analyzed by qRT-PCR. HIF1 alpha (d) and AhR (e) protein was analyzed by western blot on day 2. HIF1 alpha activity was measured using HIF1 alpha Transcription Factor Assay Kit (f). g Raw 264.7 cells were transduced with XRE/AhR Luciferase Reporter Gene Lentivirus, and treated ± butyrate (0.5 mM) 3 days post transduction. AhR activity was assessed by luciferase. h–j Cbir1 Tg CD4+ T cells were activated with APCs and Cbir1 peptide under Th1 conditions with butyrate (0.5 mM) ± YC-1 (5 µM) or/and CH-223191 (3 µM) for 60 h (n = 3/group). IL-22 mRNA (h) and protein (i) were measured by qRT-PCR and ELISA. j IL-22 was measured by flow cytometry on day 5. k WT and HIF1 alpha −/− CD4+ T cells were activated with anti-CD3/CD28 mAbs ± butyrate (0.5 mM) for 5 days (n = 3/group). IL-22 was assessed by flow cytometry. l, m CD4+ T cells were activated with anti-CD3/CD28 mAbs under Th1 conditions with or without butyrate (0.5 mM), AR420626 (5 µM), or TSA (10 nM) for 60 h (n = 3/group). Hif1a (l) and Ahr (m) were measured by qRT-PCR. One representative of three independent experiments was shown (b–m). Data were expressed as mean ± SD. Statistical significance was tested by two-tailed unpaired Student t-test (b–g) or two-tailed one-way ANOVA (h–m). b **p = 0.0033 (24 h), ***p = 0.0002 (36 h), **p = 0.0032 (48 h), *p = 0.0310 (60 h); c *p = 0.0338 (24 h), **p = 0.0054 (36 h), ***p = 0.0003 (48 h), ***p = 0.0007 (60 h); d *p = 0.0178; e *p = 0.0325; f *p = 0.0273; g *p = 0.0435; h ****p < 0.0001; i ****p < 0.0001, ***p = 0.0002 (butyrate + YC-1 vs butyrate), ***p = 0.0006; j ****p < 0.0001; k **p = 0.0015, *p = 0.0325; l **p = 0.0014 (butyrate vs control) and 0.0036 (AR429626 vs control); m ****p < 0.0001, ***p = 0.0009. Image collected and cropped by CiteAb from the following publication (, licensed under a CC-BY license. Not internally tested by R&D Systems.

Reconstitution Calculator

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.


Preparation and Storage

Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AHR

AHR (Aryl-hydrocarbon receptor; also bHLHE76) is a 100-105 kDa member of the bHLH/PAS transcription factor family. It is widely expressed and serves many functions. First, it binds multiple xenobiotic chemicals in the cytoplasm. This induces dimerization with ARNT, translocation to the nucleus, and activation of P450 genes such as CYP1A1 and UGT1A6. Second, it appears to block cell cycle progression, possibly via a downregulation of CDK proteins. And third, it blocks apoptosis by interacting with E2F1, thus silencing Tap73 and Apaf1 genes. Mouse AHR precursor is 848 amino acids (aa) in length. It contains a nine aa prosegment, plus an 839 aa mature molecule that contains a DNA binding motif (aa 12-39), a bHLH region (aa 40-80), two PAS domains (aa 116-336) and one PAC segment that stabilizes the PAS domains (aa 342-383). There are multiple alleles for mouse AHR. One 95-97 kDa allele shows a premature truncation after Ser805, while a second 112 kDa allele shows a 41 aa substitution for aa 843-848. Over aa 706-805, mouse AHR shares 87% and 63% aa identity with rat and human AHR, respectively.

Long Name
Aryl Hydrocarbon Receptor
Entrez Gene IDs
196 (Human); 11622 (Mouse); 25690 (Rat)
Alternate Names
Ah receptor; AHR; AH-receptor; aryl hydrocarbon receptor; BHLHE76; bHLHe76aromatic hydrocarbon receptor; Class E basic helix-loop-helix protein 76

Product Datasheets

You must select a language.


Citations for Mouse AHR Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

6 Citations: Showing 1 - 6
Filter your results:

Filter by:

  1. Indoleamine 2,3-dioxygenase 1 activation in mature cDC1 promotes tolerogenic education of inflammatory cDC2 via metabolic communication
    Authors: Marco Gargaro, Giulia Scalisi, Giorgia Manni, Carlos G. Briseño, Prachi Bagadia, Vivek Durai et al.
  2. Kynurenine Promotes RANKL-Induced Osteoclastogenesis In Vitro by Activating the Aryl Hydrocarbon Receptor Pathway
    Authors: Nada H. Eisa, Sakamuri V. Reddy, Ahmed M. Elmansi, Galina Kondrikova, Dmitry Kondrikov, Xing-Ming Shi et al.
    International Journal of Molecular Sciences
  3. Intestinal microbiota-derived short-chain fatty acids regulation of immune cell IL-22 production and gut immunity
    Authors: W Yang, T Yu, X Huang, AJ Bilotta, L Xu, Y Lu, J Sun, F Pan, J Zhou, W Zhang, S Yao, CL Maynard, N Singh, SM Dann, Z Liu, Y Cong
    Nat Commun, 2020-09-08;11(1):4457.
    Species: Mouse
    Sample Types: Cell Lysates
    Applications: Western Blot
  4. Kynurenine inhibits autophagy and promotes senescence in aged bone marrow mesenchymal stem cells through the aryl hydrocarbon receptor pathway
    Authors: D Kondrikov, A Elmansi, RT Bragg, T Mobley, T Barrett, N Eisa, G Kondrikova, P Schoeinlei, A Aguilar-Pe, XM Shi, S Fulzele, MM Lawrence, M Hamrick, C Isales, W Hill
    Exp. Gerontol., 2019-12-05;130(0):110805.
    Species: Mouse
    Sample Types: Whole Cells
    Applications: ICC
  5. 2,3,7,8‑tetrachlorodibenzo‑p‑dioxin suppresses the growth of human colorectal cancer cells in vitro: Implication of the aryl hydrocarbon receptor signaling
    Authors: Masayoshi Yamaguchi, Oliver Hankinson
    International Journal of Oncology
  6. 2,3,7,8‑Tetrachlorodibenzo‑p‑dioxin suppresses the growth of human liver cancer HepG2 cells in vitro: Involvement of cell signaling factors
    Authors: Masayoshi Yamaguchi, Oliver Hankinson
    International Journal of Oncology


No product specific FAQs exist for this product, however you may

View all Antibody FAQs

Reviews for Mouse AHR Antibody

Average Rating: 5 (Based on 1 Review)

5 Star
4 Star
3 Star
2 Star
1 Star

Have you used Mouse AHR Antibody?

Submit a review and receive an Amazon gift card.

$25/€18/£15/$25CAN/¥75 Yuan/¥1250 Yen for a review with an image

$10/€7/£6/$10 CAD/¥70 Yuan/¥1110 Yen for a review without an image

Submit a Review

Filter by:

Mouse AHR Antibody
By Anonymous on 10/20/2021
Application: IHC Sample Tested: Colon cancer tissue Species: Mouse