Detects mouse Angiopoietin-like Protein 6/ANGPTL6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant
human Angiopoietin-like 6 or recombinant mouse Angiopoietin-like 2 is observed.
Monoclonal Rat IgG2A Clone # 766544
Protein A or G purified from hybridoma culture supernatant
E. coli-derived recombinant mouse Angiopoietin-like Protein 6/ANGPTL6 Ala25-Leu457 Accession # Q8ROZ6
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Detection of Mouse Angiopoietin‑like Protein 6/ANGPTL6 by Western Blot. Western blot shows lysates of Neuro‑2A mouse neuroblastoma cell line, 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Angiopoietin‑like Protein 6/ANGPTL6 Monoclonal Antibody (Catalog # MAB7527) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Angiopoietin‑like Protein 6/ANGPTL6 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Angiopoietin‑like Protein 6/ANGPTL6 in BaF3 Mouse Cell Line. Angiopoietin‑like Protein 6/ANGPTL6 was detected in immersion fixed BaF3 mouse pro-B cell line using Rat Anti-Mouse Angiopoietin‑like Protein 6/ANGPTL6 Monoclonal Antibody (Catalog # MAB7527) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Sterile PBS to a final concentration of 0.5 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: Angiopoietin-like Protein 6/ANGPTL6
Angiopoietin-like 6 (ANGPTL6), also
known as angiopoietin-related growth factor (AGF), is a secreted 50 kDa protein
that contains a coiled-coil domain (aa 51-77, 126-164) and a fibrinogen-like
domain (aa 238-456). A conserved Integrin-binding RGD motif is located within
the fibrinogen-like domain. This enables ANGPTL6 to promote skin wound healing
by mediating the adhesion and migration of keratinocytes, fibroblasts, and
endothelial cells. ANGPTL6 also promotes the chemotaxis of vascular endothelial
cells resulting in increased vascular permeability and angiogenesis. ANGPTL6 is
also secreted by hepatocytes. It inhibits gluconeogenesis in these cells and
promotes insulin sensitivity and energy expenditure in mice fed high fat diets.
Serum levels of ANGPTL6 are elevated in metabolic syndrome, diabetes, and
preeclampsia but are decreased in chronic renal failure. ANGPTL6 is
additionally produced by several hematopoietic cell types including
megakaryocytes, platelets, mast cells, and uterine NK cells. Mature mouse
ANGPTL6 shares 75% and 95% sequence identity with human and rat ANGPTL6.
Entrez Gene IDs:
83854 (Human); 70726 (Mouse); 298698 (Rat)
AGF; AGFAngiopoietin-related protein 5; Angiopoietin like Protein 6; angiopoietin-like 6; Angiopoietin-like protein 6; Angiopoietin-related growth factor; ANGPTL6; ARP5; ARP5angiopoietin-related protein 6
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.