Key Product Details

Species Reactivity

Mouse

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2A Clone # 766544
View all Angiopoietin-like Protein 6/ANGPTL6 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant mouse Angiopoietin-like Protein 6/ANGPTL6
Ala25-Leu457
Accession # Q8ROZ6

Specificity

Detects mouse Angiopoietin-like Protein 6/ANGPTL6 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human Angiopoietin-like 6 or recombinant mouse Angiopoietin-like 2 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse Angiopoietin‑like Protein 6/ANGPTL6 Antibody

Detection of Mouse Angiopoietin-like Protein 6/ANGPTL6 antibody by Western Blot.

Detection of Mouse Angiopoietin‑like Protein 6/ANGPTL6 by Western Blot.

Western blot shows lysates of Neuro-2A mouse neuroblastoma cell line, 3T3-L1 mouse embryonic fibroblast adipose-like cell line, and J774A.1 mouse reticulum cell sarcoma macrophage cell line. PVDF membrane was probed with 0.5 µg/mL of Rat Anti-Mouse Angiopoietin-like Protein 6/ANGPTL6 Monoclonal Antibody (Catalog # MAB7527) followed by HRP-conjugated Anti-Rat IgG Secondary Antibody (Catalog # HAF005). A specific band was detected for Angiopoietin-like Protein 6/ANGPTL6 at approximately 52 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Angiopoietin-like Protein 6/ANGPTL6 antibody in BaF3 Mouse Cell Line by Immunocytochemistry (ICC).

Angiopoietin‑like Protein 6/ANGPTL6 in BaF3 Mouse Cell Line.

Angiopoietin-like Protein 6/ANGPTL6 was detected in immersion fixed BaF3 mouse pro-B cell line using Rat Anti-Mouse Angiopoietin-like Protein 6/ANGPTL6 Monoclonal Antibody (Catalog # MAB7527) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Rat IgG Secondary Antibody (red; Catalog # NL013) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse Angiopoietin‑like Protein 6/ANGPTL6 Antibody

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed BaF3 mouse pro-B cell line

Western Blot

0.5 µg/mL
Sample: Neuro‑2A mouse neuroblastoma cell line, 3T3‑L1 mouse embryonic fibroblast adipose-like cell line, and J774A.1 mouse reticulum cell sarcoma macrophage cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Angiopoietin-like Protein 6/ANGPTL6

Angiopoietin-like 6 (ANGPTL6), also known as angiopoietin-related growth factor (AGF), is a secreted 50 kDa protein that contains a coiled-coil domain (aa 51-77,
126-164) and a fibrinogen-like domain (aa 238-456). A conserved Integrin-binding RGD motif is located within the fibrinogen-like domain. This enables ANGPTL6 to promote skin wound healing by mediating the adhesion and migration of keratinocytes, fibroblasts, and endothelial cells. ANGPTL6 also promotes the chemotaxis of vascular endothelial cells resulting in increased vascular permeability and angiogenesis. ANGPTL6 is also secreted by hepatocytes. It inhibits gluconeogenesis in these cells and promotes insulin sensitivity and energy expenditure in mice fed high fat diets. Serum levels of ANGPTL6 are elevated in metabolic syndrome, diabetes, and preeclampsia but are decreased in chronic renal failure. ANGPTL6 is additionally produced by several hematopoietic cell types including megakaryocytes, platelets, mast cells, and uterine NK cells. Mature mouse ANGPTL6 shares 75% and 95% sequence identity with human and rat ANGPTL6.

Alternate Names

AGF, Angiopoietin like Protein 6, ANGPTL6, ARP5

Entrez Gene IDs

83854 (Human); 70726 (Mouse); 298698 (Rat)

Gene Symbol

ANGPTL6

UniProt

Q8ROZ6

Additional Angiopoietin-like Protein 6/ANGPTL6 Products

Product Documents for Mouse Angiopoietin‑like Protein 6/ANGPTL6 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse Angiopoietin‑like Protein 6/ANGPTL6 Antibody

For research use only

Citations for Mouse Angiopoietin‑like Protein 6/ANGPTL6 Antibody

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Protocols

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