Mouse BAFF/BLyS/TNFSF13B Biotinylated Antibody

(4 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse BAFF/BLyS/TNFSF13B in Western blots. In Western blots, approximately 40% cross‑reactivity with recombinant human (rh) BAFF is observed, 10% cross-reactivity with recombinant mouse (rm) LIGHT and rhEDA-A2 is observed and less than 1% cross‑reactivity with rmTWEAK, rhAPRIL, rmFas Ligand, rhGITR Ligand, rmOX40 Ligand, rmTNF-a, rmTRAIL and rm4-1BB Ligand is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse BAFF/BLyS/TNFSF13B
    Ala127-Leu309
    Accession # Q9WU72
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse BAFF/BLyS/TNFSF13B (Catalog # 2106-BF)
  • Immunocytochemistry
    5-15 µg/mL
    Immersion fixed mouse splenocytes
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BAFF/BLyS/TNFSF13B

BAFF (also known as TALL-1, BLyS, THANK) is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated as TNF superfamily member 13B (TNFSF13B). Mouse BAFF is a 309 aa protein consisting of a 248 aa extracellular domain, a 21 aa transmembrane region and a 45 aa cytoplasmic tail (1, 2). BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain (3, 4). A higher ordered structure composed of a cluster of trimeric units resembling the structure of a viral capsid has also been reported (4). Mouse BAFF may be shed from the cell surface by proteolytic cleavage between R126 and Ala 127 to yield a soluble form of the protein detectable in serum (1, 5). Within the TNF superfamily BAFF shares the highest homology (48%) with APRIL (1). BAFF shares with APRIL the ability to bind to BCMA and TACI and also binds specifically to BAFF receptor (BAFF R, also known as BR3 or TNFSFR13C), which is the principal BAFF receptor (6‑8). All three receptors are type III transmembrane proteins that are expressed in B cells. BAFF and APRIL can form active heteromers that bind TACI (9). BAFF is expressed in peripheral blood mononuclear cells, in spleen and lymph nodes. Its expression in resting monocytes is upregulated by IFN-alpha, IFN-beta, LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response (10). BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells (7, 11). Human and mouse BAFF share 86% aa sequence identity (1).

  • References:
    1. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
    2. Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978.
    3. Karpusas, M. et al. (2002) J. Mol. Biol. 315:1145. 
    4. Liu, Y. et al. (2002) Cell 108:383. 
    5. Cheema, G.S. et al. (2001) Arthr. Rheum. 44:1313.
    6. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
    7. Thompson, J.S. et al. (2001) Science 293:2108.
    8. Ng, L. G. et al. (2004) J. Immunol. 173:807.
    9. Roschke, V. et al. (2002) J. Immunol. 169:4314.
    10. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
    11. Avery, D.T. et al. (2003) J. Clin. Invest. 112:286.
  • Long Name:
    B cell Activating Factor
  • Entrez Gene IDs:
    10673 (Human); 24099 (Mouse); 498666 (Rat)
  • Alternate Names:
    ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

4 Citations: Showing 1 - 4
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Species
Applications
Sample Type
  1. Regulation of type 1 diabetes development and B-cell activation in nonobese diabetic mice by early life exposure to a diabetogenic environment
    Authors: A De Riva, M Wållberg, F Ronchi, R Coulson, A Sage, L Thorne, I Goodfellow, KD McCoy, M Azuma, A Cooke, R Busch
    PLoS ONE, 2017;12(8):e0181964.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA detection
  2. Interferon regulatory factor-5 deficiency ameliorates disease severity in the MRL/lpr mouse model of lupus in the absence of a mutation in DOCK2.
    Authors: Yasuda K, Watkins A, Kochar G, Wilson G, Laskow B, Richez C, Bonegio R, Rifkin I
    PLoS ONE, 2016;9(7):e103478.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA detection
  3. Myeloid cells, BAFF, and IFN-gamma establish an inflammatory loop that exacerbates autoimmunity in Lyn-deficient mice.
    Authors: Scapini P, Hu Y, Chu CL
    J. Exp. Med., 2010;207(8):1757-73.
    Species: Mouse
    Sample Type: Serum
    Application: ELISA Development
  4. The sensing of environmental stimuli by follicular dendritic cells promotes immunoglobulin A generation in the gut.
    Authors: Suzuki K, Maruya M, Kawamoto S
    Immunity, 2010;33(1):71-83.
    Species: Mouse
    Sample Type: Whole Tissue
    Application: IHC Frozen
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