hh Mouse BAFF/BLyS/TNFSF13B Antibody AF2106: R&D Systems

Mouse BAFF/BLyS/TNFSF13B Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse BAFF/BLyS/TNFSF13B in direct ELISAs and Western blots. In direct ELISAs, approximately 30% cross-reactivity with recombinant human (rh) BAFF, 10% cross-reactivity with recombinant mouse (rm) LIGHT, rhEDA‑A2, and rhTRANCE and less than 1% cross‑reactivity with rmTWEAK, rhAPRIL, rmFas Ligand, rhGITR Ligand, rmOX40 Ligand, rmTNF‑ alpha, rmTRAIL, and rm4‑1BB Ligand is observed.
  • Source
    Polyclonal Goat IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse BAFF/BLyS/TNFSF13B
    Ala127-Leu309
    Accession # Q9WU72
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    0.1 µg/mL
    Recombinant Mouse BAFF/BLyS/TNFSF13B (Catalog # 2106-BF)
  • Neutralization
    Measured by its ability to neutralize BAFF/BLyS/TNFSF13B-induced proliferation in mouse B cells. The Neutralization Dose (ND50) is typically 0.01-0.04 µg/mL in the presence of 3 ng/mL Recombinant Mouse BAFF/BLyS/TNFSF13B and 10 µg/mL Goat F(ab')2 Anti-mouse IgM.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Cell Proliferation Induced by BAFF/BLyS/TNFSF13B and Neutralization by Mouse BAFF/BLyS/TNFSF13B Antibody. In the presence of Goat F(ab')2 Anti-mouse IgM, Recombinant Mouse BAFF/BLyS/
TNFSF13B (Catalog # 2106-BF) stimulates pro­liferation in mouse B cells in a dose-dependent manner (orange line). Under these conditions, proliferation elicited by Recombinant Mouse BAFF/BLyS/TNFSF13B (3 ng/mL) is neutralized (green line) by increasing concentrations of Mouse BAFF/BLyS/TNFSF13B Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2106). The ND50 is typically 0.01‑0.04 µg/mL.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.2 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BAFF/BLyS/TNFSF13B

BAFF (also known as TALL-1, BLyS, THANK) is a type II transmembrane glycoprotein belonging to the TNF superfamily and has been designated as TNF superfamily member 13B (TNFSF13B). Mouse BAFF is a 309 aa protein consisting of a 248 amino acid (aa) extracellular domain, a 21 aa transmembrane region and a 45 aa cytoplasmic tail (1, 2). BAFF has the typical structural characteristics of the TNF superfamily ligands. It is a homotrimeric protein having the structurally conserved motif known as TNF homology domain (3, 4). A higher ordered structure composed of a cluster of trimeric units resembling the structure of a viral capsid has also been reported (4). Mouse BAFF may be shed from the cell surface by proteolytic cleavage between R126 and Ala 127 to yield a soluble form of the protein detectable in serum (1, 5). Within the TNF superfamily BAFF shares the highest homology (48%) with APRIL (1). BAFF shares with APRIL the ability to bind to BCMA and TACI and also binds specifically to BAFF receptor (BAFF R, also known as BR3 or TNFSFR13C), which is the principal BAFF receptor (6-8). All three receptors are type III transmembrane proteins that are expressed in B cells. BAFF and APRIL can form active heteromers that bind TACI (9). BAFF is expressed in peripheral blood mononuclear cells, in spleen and lymph nodes. Its expression in resting monocytes is upregulated by IFN-alpha, IFN-beta, LPS and IL-10. BAFF provides critical survival signals to a subset of B cells with intermediate maturation status (T2 B cells) during the immune response (10). BAFF also plays an important role in the development of lymphoid tissue and enhances the survival of activated memory B cells (7, 11). Human and mouse BAFF share 86% aa sequence identity (1).

  • References:
    1. Schneider, P. et al. (1999) J. Exp. Med. 189:1747.
    2. Mukhopadhyay, A. et al. (1999) J. Biol. Chem. 274:15978.
    3. Karpusas, M. et al. (2002) J. Mol. Biol. 315:1145. 
    4. Liu, Y. et al. (2002) Cell 108:383. 
    5. Cheema, G.S. et al. (2001) Arthr. Rheum. 44:1313. 
    6. Marsters, S.A. et al. (2000) Curr. Biol. 10:785.
    7. Thompson, J.S. et al. (2001) Science 293:2108.
    8. Ng, L. G. et al. (2004) J. Immunol. 173:807.
    9. Roschke, V. et al. (2002) J. Immunol. 169:4314.
    10. Batten, M. et al. (2000) J. Exp. Med. 192:1453.
    11. Avery, D.T. et al. (2003) J. Clin. Invest. 112:286.
  • Long Name:
    B cell Activating Factor
  • Entrez Gene IDs:
    10673 (Human); 24099 (Mouse); 498666 (Rat)
  • Alternate Names:
    ApoL related ligand TALL-1; B lymphocyte stimulator; BAFF; BAFFB-cell activating factor; B-cell-activating factor; BLyS; BLYSB-lymphocyte stimulator; CD257 antigen; CD257; Dendritic cell-derived TNF-like molecule; DTL; TALL1; TALL-1delta BAFF; TALL1Delta4 BAFF; THANK; TNF- and APOL-related leukocyte expressed ligand 1; TNF and ApoL-related leukocyte expressed ligand 1; TNF homolog that activates apoptosis; TNFSF13B; TNFSF20; tumor necrosis factor (ligand) superfamily, member 13b; tumor necrosis factor (ligand) superfamily, member 20; tumor necrosis factor ligand superfamily member 13B; tumor necrosis factor superfamily, member 13b; tumor necrosis factor-like protein ZTNF4; ZTNF4
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Neutrophils license iNKT cells to regulate self-reactive mouse B cell responses
    Nat. Immunol., 2016;0(0):.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  2. The BAFF receptor TACI controls IL-10 production by regulatory B cells and CLL B cells.
    Authors: Saulep-Easton D, Vincent F, Quah P, Wei A, Ting S, Croce C, Tam C, Mackay F
    Leukemia, 2016;30(1):163-72.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Neut
  3. Functional implication of BAFF synthesis and release in gangliosides-stimulated microglia.
    Authors: Kim KS, Park JY, Jou I, Park SM
    J. Leukoc. Biol., 2009;86(2):349-59.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
Isotype Controls
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