Mouse BAFF R/TNFRSF13C Antibody

(3 citations)   
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse BAFF R/TNFRSF13C in direct ELISAs. In direct ELISAs, 100% cross-reactivity with recombinant human (rh) BAFF R is observed, approximately 10‑25% cross-reactivity with recombinant mouse (rm) 4‑1BB, rmDR3, rhDR6, rmEDAR, rhHVEM, rmNGF R, rmOX40, and rmTROY is observed, and no cross-reactivity with rmCD27, rmCD30, rmCD40, rmFAS, rmGITR, rmLymphotoxin  beta R, rmOPG, rmRANK, rmTNFRI, or rmTNF RII is observed.
  • Source
    Monoclonal Rat IgG2A Clone # 204406
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    Mouse myeloma cell line NS0-derived recombinant mouse BAFF R/TNFRSF13C
    Gly2-Ala71
    Accession # Q9D8D0
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Endotoxin Level
    <0.10 EU per 1 μg of the antibody by the LAL method.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    0.25 µg/106 cells
    See below
  • CyTOF-ready
    Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of BAFF R/TNFRSF13C in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse BAFF R/TNFRSF13C Monoclonal Antibody (Catalog # MAB1755) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Fluorescein-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0104B).
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: BAFF R/TNFRSF13C

B-cell activating factor (BAFF), also known as BlyS, TALL-1, TNAK, and zTNF4, is a TNF ligand superfamily member and has been designated TNFSF13B. It is produced by macrophages, dendritic cells, and T lymphocytes. BAFF promotes the survival of B cells and is essential for B cell maturation (1‑4). BAFF binds to three TNF receptor superfamily members: B‑cell maturation antigen (BCMA/TNFRSF17), transmembrane activator and calcium-modulator and cyclophilin ligand interactor (TACI/TNFRSF13B) and BAFF receptor (BAFF R/BR3/TNFRSF 13C). These receptors are type III transmembrane proteins that lack a signal peptide. Whereas TACI and BCMA bind BAFF and another TNF superfamily ligand, APRIL(a proliferation-inducing ligand), BAFF-R selectively binds BAFF. Mouse BAFF-R cDNA encodes a 175 amino acid residue (aa) transmembrane protein with a 71 aa extracellular domain, a 21 aa transmembrane domain, and a 83 aa cytoplasmic region. A second isoform of BAFF R that has a 72 aa cytoplamic region can also be produced by alternative splicing. The BAFF R extracellular domain lacks the TNF receptor canonical cysteine-rich domain (CRD) and contains only a partial CRD with four cysteine residues. Human and mouse BAFF R share 56% aa sequence identity. BAFF R is highly expressed in spleen, lymph node and resting B cells. It is also expressed at lower levels in activated B cells, in resting CD4+ T cells, in thymus and peripheral blood leukocytes. BAFF knockout mice lack mature B cells and has profound defects in antibody mediated immune responses. Similarly, A/WySnJ mice that are defective in BAFF R intracellular signaling also lack mature B cells, suggesting that BAFF R is the critical receptor for BAFF during B lymphopoiesis. In contrast, BCMA- or TACI-deficient mice have no major defect in B‑cell development. While the function of BCMA is not defined, TACI has been shown to control
B-cell homeostasis and T-cell-independent immune responses.

  • References:
    1. Rolink, A.G. and F. Melcher (2002) Curr. Opin. Immunol. 14:266.
    2. Mackay, F. and J.L. Browning (2002) Nature Reviews Immunology 2:464.
    3. Laabi, Y. et al. (2001) Current Biol. 11:R1013.
    4. Thompson, J.S. et al. (2001) Science 14:2108.
  • Long Name:
    B cell Activating Factor Receptor
  • Entrez Gene IDs:
    115650 (Human); 72049 (Mouse)
  • Alternate Names:
    BAFF R; BAFFR; BR3; CD268; TNFRSF13C
Related Research Areas
Citations:

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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Species
Applications
Sample Type
  1. Cutting edge: the dependence of plasma cells and independence of memory B cells on BAFF and APRIL.
    Authors: Benson MJ, Dillon SR, Castigli E, Geha RS, Xu S, Lam KP, Noelle RJ
    J. Immunol., 2008;180(6):3655-9.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  2. Direct B cell stimulation by dendritic cells in a mouse model of lupus.
    Authors: Wan S, Zhou Z, Duan B, Morel L
    Arthritis Rheum., 2008;8(0):1741-50.
    Species: Mouse
    Sample Type: Whole Cells
    Application: Flow
  3. In vitro and in vivo activation induces BAFF and APRIL expression in B cells.
    Authors: Chu VT, Enghard P, Riemekasten G, Berek C
    J. Immunol., 2007;179(9):5947-57.
    Species: Mouse
    Sample Type: Whole Cells
    Application: ICC
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2A Isotype Control

Ctrl MAB006 90  
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Normal Rat IgG Control

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Normal Rat IgG Control (Azide Free)

Ctrl 6-001-F 1
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Rat IgG2A Isotype Control

Ctrl MAB006R  
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Secondary Antibodies
Description Application Cat# Citations Images  

Rat IgG HRP-conjugated Antibody

WB, Simple Western HAF005 7  
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Goat Anti-Rat IgG NorthernLights™ NL557-conjugated Antibody

Flow, IHC, ICC NL013 2
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Goat Anti-Rat F(ab)2 IgG (H+L)
APC-conjugated Antibody

Flow F0113 2  
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Rat F(ab)2 IgG (H+L) PE-conjugated Antibody

Flow F0105B 2  
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Goat Anti-Rat IgG NorthernLights™ NL493-conjugated Antibody

Flow, IHC, ICC NL015 1
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Goat Anti-Rat IgG Biotinylated Antibody

WB BAF005 2
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Rat IgG VisUCyte HRP Polymer Antibody

IHC VC005  
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Goat Anti-Rat IgG Antibody

WB AF005 1
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Rat F(ab)2 IgG (H+L) Fluorescein-conjugated Antibody

Flow F0104B  
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Goat Anti-Rat IgG NorthernLights™ NL637-conjugated Antibody

Flow, IHC, ICC NL014
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Goat Anti-Rat F(ab)2 IgG (H+L) PerCP-conjugated Antibody

Flow F0115 1
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