Mouse beta -NGF Antibody Summary
Accession # Q6LDU8
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This antibody functions as an ELISA capture antibody when paired with Rabbit Anti-Mouse beta ‑NGF Monoclonal Antibody (Catalog # MAB11561 ).
This product is intended for assay development on various assay platforms requiring antibody pairs.
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Mouse beta ‑NGF ELISA Standard Curve. Recombinant Mouse beta -NGF protein was serially diluted 2-fold and captured by Rabbit Anti-Mouse beta -NGF Monoclonal Antibody (Catalog # MAB1156) coated on a Clear Polystyrene Microplate (Catalog # DY990). Rabbit Anti-Mouse beta -NGF Monoclonal Antibody (Catalog # MAB11561) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998) followed by Substrate Solution (Catalog # DY999) and stopping the enzymatic reaction with Stop Solution (Catalog # DY994).
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
NGF was discovered as a molecule that promoted the survival and differentiation of sympathetic and sensory neurons in the peripheral nervous system (1). In addition, beta -NGF can act in the central nervous system as a trophic factor for basal forebrain cholinergic neurons (2). beta -NGF has also been shown to have biological effects on a variety of neurons, glia, and nonneural cells (3). NGF was initially isolated from the mouse submandibular gland as a 7S complex composed of three non-covalently linked subunits, alpha, beta, and gamma. It is now known that both the alpha and gamma subunits of NGF are members of the kallikrein family of serine proteases while the beta subunit, called beta -NGF, exhibits all the biological activities ascribed to NGF (4 - 5). Recombinant mouse beta -NGF is a homodimer of two 120 amino acid polypeptides. It shares approximately 90% homology at the amino acid level with human beta -NGF and 95.8% with rat beta -NGF (6).
NGF is the first member discovered in the Neurotrophin family, which includes brain-derived neurotrophic factor (BDNF), neurotrophin-3 (NT-3), and neurotrophin-4 (NT-4). NT-6 and NT-7 were also identified in fish (7, 8). Biological activities of the NGF family are mediated through binding and activation of two types of receptors, TrKs (TrKA, TrKB, and TrKC) and NGF receptor. TrKs are single-pass membrane proteins belonging to the receptor tyrosine kinase family. The neurotrophins bind to TrKs specifically. NGF is specific for TrKA, BDNF and NT-4 for TrKB, and NT-3 mostly for TrKC. NGF receptor is a TNF receptor superfamily protein that binds to all the neurotrophins with similar affinity (9). TrkA is often referred to as the high-affinity receptor. However, most high affinity binding is mediated by the interaction of NGF receptor with TrKA. Both NGF and its receptors are expressed during development, adult life and aging in many cell types in CNS and PNS, immune and inflammatory systems. NGF signaling has been shown to play an important role in neuroprotection and repair (3, 10).
- Levi-Montalcini, R and V. Hamberger (1951) J. Exp. Zool. 116:321.
- Levi-Montacini, R. Ann. Rev. Neurosci. 5:341.
- Sofroniew, M.V. et al. (2001) Ann Rev Neurosci. 24:1217.
- Edwards, R.H. et al. (1988) J Biol Chem. 263:6810.
- Angeletti, R H. et al. (1973) Biochemistry 12:100.
- Ullrich, A. et al. (1983) Nature 303:821.
- Gotz, R. et al. (1994) Nature 372:266.
- Lai, K.O. et al. (1998) Mol. Cell. Neurosci. 11:64.
- Bothwell, M. (1995) Ann Rev Neurosci. 18:223.
- E.J. Huang and L.F. Reichardt (2001) Ann Rev Neurosci. 24:677.
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