Detection of BST-2/Tetherin in Mouse Bone Marrow Cells by Flow Cytometry.
Mouse bone marrow cells were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse BST-2/Tetherin Monoclonal Antibody (Catalog # MAB8660) or (B) Rat IgG2A Isotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
BST-2/Tetherin in Mouse Splenocytes.
BST-2/Tetherin was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse BST-2/Tetherin Monoclonal Antibody (Catalog # MAB8660) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
Reconstitute at 0.5 mg/mL in sterile PBS.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
BST-2, also known as Tetherin or PDCA1 and designated CD317, is a 30-35 kDa interferon-inducible protein that shows an unusual topology. The N-terminus is cytoplasmic, followed by a transmembrane segment, an extracellular loop, and a C-terminal GPI-linkage. BST-2 is expressed on bone marrow stromal cells and is upregulated in breast cancer and astrocytoma. It binds to ILT7 on plasmacytoid dendritic cells and inhibits proinflammatory TLR7 and TLR9 signaling. BST-2 inhibits the release of Kaposi sarcoma virus, HIV-1, and Lassa virus from infected cells, but this function is counteracted by viral proteins which directly bind and trigger the degradation of BST-2.
Submit a review and receive a $25US/€18/£15/$25CAN amazon gift card if you include an image - $10US/€7/£6/$10CAN Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.