Mouse BST-2/Tetherin Antibody (44E9R)

R&D Systems | Catalog # MAB8660

Recombinant Monoclonal Antibody.
R&D Systems
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Key Product Details

Species Reactivity

Validated:

Mouse

Cited:

Mouse

Applications

Validated:

Flow Cytometry, Immunocytochemistry, CyTOF-ready

Cited:

Immunohistochemistry, Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rat IgG2A Clone # 44E9R
View all BST-2/Tetherin Primary Antibodies »

Product Specifications

Immunogen

Recombinant extracellular domain of CD317

Specificity

Detects mouse BST-2/Tetherin in flow cytometry.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2A

Scientific Data Images for Mouse BST-2/Tetherin Antibody (44E9R)

Detection of BST-2/Tetherin antibody in Mouse Bone Marrow Cells antibody by Flow Cytometry.

Detection of BST-2/Tetherin in Mouse Bone Marrow Cells by Flow Cytometry.

Mouse bone marrow cells were stained with Rat Anti-Mouse B220/CD45R APC-conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse BST-2/Tetherin Monoclonal Antibody (Catalog # MAB8660) or (B) Rat IgG2AIsotype Control (Catalog # MAB006) followed by Phycoerythrin-conjugated Anti-Rat IgG Secondary Antibody (Catalog # F0105B).
BST-2/Tetherin antibody in Mouse Splenocytes by Immunocytochemistry (ICC).

BST-2/Tetherin in Mouse Splenocytes.

BST-2/Tetherin was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse BST-2/Tetherin Monoclonal Antibody (Catalog # MAB8660) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Mouse BST-2/Tetherin Antibody (44E9R)

Application
Recommended Usage

CyTOF-ready

Ready to be labeled using established conjugation methods. No BSA or other carrier proteins that could interfere with conjugation.

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse bone marrow cells

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed mouse splenocytes

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from cell culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BST-2/Tetherin

BST-2, also known as Tetherin or PDCA1 and designated CD317, is a 30-35 kDa interferon-inducible protein that shows an unusual topology. The N-terminus is cytoplasmic, followed by a transmembrane segment, an extracellular loop, and a C-terminal GPI-linkage. BST-2 is expressed on bone marrow stromal cells and is upregulated in breast cancer and astrocytoma. It binds to ILT7 on plasmacytoid dendritic cells and inhibits proinflammatory TLR7 and TLR9 signaling. BST-2 inhibits the release of Kaposi sarcoma virus, HIV-1, and Lassa virus from infected cells, but this function is counteracted by viral proteins which directly bind and trigger the degradation of BST-2. 

Long Name

Bone Marrow Stromal Cell Antigen 2

Alternate Names

BST2, CD317, HM1.24 Antigen, NPC-A-7, Tetherin

Entrez Gene IDs

684 (Human); 69550 (Mouse)

Gene Symbol

BST2

Additional BST-2/Tetherin Products

Product Documents for Mouse BST-2/Tetherin Antibody (44E9R)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse BST-2/Tetherin Antibody (44E9R)

For research use only

Citations for Mouse BST-2/Tetherin Antibody (44E9R)

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Protocols

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