Key Product Details

Species Reactivity

Mouse

Applications

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all BTN1A1/Butyrophilin Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant mouse BTN1A1/Butyrophilin
Ala27-Trp247
Accession # Q62556

Specificity

Detects mouse BTN1A1/Butyrophilin in direct ELISAs and Western blots. In direct ELISAs, approximately 25% cross-reactivity with recombinant human BTN1A1/Butyrophilin is observed.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Mouse BTN1A1/Butyrophilin Antibody

Detection of Mouse BTN1A1/Butyrophilin antibody by Western Blot.

Detection of Mouse BTN1A1/Butyrophilin by Western Blot.

Western blot shows lysates of mouse lactating mammary tissue. PVDF membrane was probed with 0.25 µg/mL of Sheep Anti-Mouse BTN1A1/Butyrophilin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF4765) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for BTN1A1/Butyrophilin at approximately 66 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Mouse BTN1A1/Butyrophilin Antibody

Application
Recommended Usage

Western Blot

0.25 µg/mL
Sample: Mouse lactating mammary tissue

Reviewed Applications

Read 1 review rated 5 using AF4765 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: BTN1A1/Butyrophilin

Butyrophilin (Greek for “being like butter”; also Btn1a1) is a 66 kDa member of the BTN/MOG family of proteins. It is expressed on secretory mammary epithelium and associates with xanthine dehydrogenase. This is necessary for milk-lipid droplet secretion. Mature mouse Butyrophilin is 498 amino acids (aa) in length. It is a type I transmembrane glycoprotein that contains a 221 aa extracellular domain (ECD) (aa 27‑247) and a 256 aa cytoplasmic region. The ECD contains two V-type Ig‑like domains (aa 29‑141 and aa 149‑235). There are two potential splice variants. One shows a deletion of aa 145‑238, while a second shows a 36 aa substitution for aa 144‑524. Over aa 27‑247, mouse Butyrophilin shares 85% and 67% aa identity with rat and human Butyrophilin, respectively.

Alternate Names

BTN, BTN1A1

Entrez Gene IDs

696 (Human); 12231 (Mouse); 306956 (Rat)

Gene Symbol

BTN1A1

UniProt

Q62556 (Mouse)

Additional BTN1A1/Butyrophilin Products

Product Documents for Mouse BTN1A1/Butyrophilin Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Mouse BTN1A1/Butyrophilin Antibody

For research use only

Customer Reviews for Mouse BTN1A1/Butyrophilin Antibody (1)

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  • Mouse BTN1A1/Butyrophilin Antibody
    Name: Anonymous
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: Lactating mammary tissue
    Species: Mouse
    Verified Customer | Posted 05/26/2023
    Day 6 lactating mouse mammary gland tissue. Antigen retrieval with Tris EDTA (pH 9). Blocked with 50% serum, permeabolized with 0.3% TritonX-100. Used 1:50 dilution of anti-Btn1a1, @RT 1 hour. Detection was with anti-sheep HRP (1:200; 1hour RT) then anti-HRP Alexa Fluor 488 (1:50; 1hour RT). Nuclei stained with DAPI.
    Mouse BTN1A1/Butyrophilin Antibody AF4765

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