Detects mouse Cadherin-4/R-Cadherin in direct ELISAs and Western blots. In direct ELISAs, approximately 5% cross-reactivity with recombinant human Cadherin-4/R-Cadherin and recombinant mouse (rm) Cadherin-2 is observed and less than 1% cross-reactivity with rmE‑Cadherin is observed.
Detection of Mouse Cadherin‑4/R‑Cadherin by Western Blot.
Western blot shows lysates of mouse brain (cortex) tissue. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse Cadherin‑4/R‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6677) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for Cadherin‑4/R‑Cadherin at approximately 100 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Cadherin‑4/R‑Cadherin in Mouse Brain.
Cadherin‑4/R‑Cadherin was detected in immersion fixed frozen sections of embryonic mouse brain using Sheep Anti-Mouse Cadherin‑4/R‑Cadherin Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6677) at 1.7 µg/mL overnight at 4 °C. Tissue was stained using the Anti-Sheep HRP-DAB Cell & Tissue Staining Kit (brown; Catalog # CTS019) and counterstained with hematoxylin (blue). Specific staining was localized to developing brain. View our protocol for Chromogenic IHC Staining of Frozen Tissue Sections.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
Reconstitution Buffer Available
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
Cadherin-4 (also Retinal/R-Cadherin) is a 120 kDa (100 kDa predicted) member of the classical cadherin family of molecules. It is expressed on mammary epithelium, olfactory bulb neurons, cerebellar granule cells, vascular smooth muscle, astrocytes and pancreatic beta -cells. Cadherin-4 forms both homodimers and heterodimers with N-cadherin. These complexes will oligomerize, providing sources for adhesion and migration. Cadherin-4 also binds to KLRG1 expressed on NK, T and Treg lymphocytes. Mouse cadherin-4 proprecursor is 893 amino acids (aa) in length. It contains a 146 aa propeptide (aa 21-166) coupled to a 746 amino acid (aa) type I transmembrane mature protein (aa 167-913). The mature region possesses a 565 aa extracellular domain (aa 167-731) plus a 160 aa cytoplasmic region (aa 753-913). Consistent with classical cadherins, cadherin-4 contains five cadherin repeats in the extracellular domain (aa 167-721). There is one potential splice form that shows a 44 aa substitution for aa 291-913. Over aa 21-731, mouse cadherin-4 shares 98% and 93% aa identity with rat and human cadherin-4, respectively.
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