Detection of CD3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R PE‑conjugated Monoclonal Antibody (Catalog # FAB1217P) and either (A) Rat Anti-Mouse CD3 Alexa Fluor® 647‑conjugated Monoclonal Antibody (Catalog # FAB4841R) or (B) Rat IgG2B Alexa Fluor 647 Isotype Control (Catalog # IC013R). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Monoclonal 17A2 was generated by immunizing rats with a T-cell hybridoma. This antibody has been shown to react with the epsilon chain of the CD3 complex. The CD3 protein complex is expressed on thymocytes and mature T-cells and, therefore, is a useful reagent to monitor T-cell frequencies in tissues.
The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.