Detection of CD3 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse CD3 Alexa Fluor® 700‑conjugated Monoclonal Antibody (Catalog # FAB4841N, filled histogram) or isotype control antibody (Catalog # IC013N, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Monoclonal 17A2 was generated by immunizing rats with a T-cell hybridoma (1). This antibody has been shown to react with the epsilon chain of the CD3 complex (1). The CD3 protein complex is expressed on thymocytes and mature T-cells and, therefore, is a useful reagent to monitor T-cell frequencies in tissues.
Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
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