Mouse CD3 Alexa Fluor® 405-conjugated Antibody

  • Species Reactivity
  • Specificity
    Reacts with mouse TCR-associated CD3 complex that occurs on thymocytes and mature T cells.
  • Source
    Monoclonal Rat IgG2B Clone # 17A2
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    T cell hybridoma D1
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 405
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD3 in Mouse Splenocytes by Flow Cytometry. Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse CD3 Alexa Fluor® 405‑conjugated Monoclonal Antibody (Catalog # FAB4841V) or (B) Rat IgG2B Alexa Fluor 405 Isotype Control (Catalog # IC013V). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD3
Monoclonal 17A2 was generated by immunizing rats with a T-cell hybridoma. This antibody has been shown to react with the epsilon chain of the CD3 complex. The CD3 protein complex is expressed on thymocytes and mature T-cells and, therefore, is a useful reagent to monitor T-cell frequencies in tissues.
  • Entrez Gene IDs:
    916 (Human); 12503 (Mouse)
  • Alternate Names:
    CD3 antigen, delta subunit; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Rat IgG2B Alexa Fluor® 405-conjugated Isotype Control

Ctrl IC013V
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Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
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Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
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Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
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