Detection of CD3 in Mouse Splenocytes by Flow Cytometry.
Mouse splenocytes were stained with Rat Anti-Mouse B220/CD45R APC‑conjugated Monoclonal Antibody (Catalog # FAB1217A) and either (A) Rat Anti-Mouse CD3 Alexa Fluor® 594‑conjugated Monoclonal Antibody (Catalog # FAB4841T) or (B) Rat IgG2B Alexa Fluor 594 Isotype Control (Catalog # IC013T). View our protocol for Staining Membrane-associated Proteins.
CD3 in Mouse Splenocytes.
CD3 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD3 Alexa Fluor® 594‑conjugated Monoclonal Antibody (red; Catalog # FAB4841T) at 10 µg/mL for 3 hours at room temperature. Cells were counterstained with DAPI (blue). Specific staining was localized to cell surfaces and cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Protect from light. Do not freeze.
12 months from date of receipt, 2 to 8 °C as supplied.
Monoclonal 17A2 was generated by immunizing rats with a T-cell hybridoma. This antibody has been shown to react with the epsilon chain of the CD3 complex. The CD3 protein complex is expressed on thymocytes and mature T-cells and, therefore, is a useful reagent to monitor T-cell frequencies in tissues.
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