Mouse CD3 Biotinylated Antibody BAM4841: R&D Systems

Mouse CD3 Biotinylated Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD3. Recognizes mouse T cell receptor-associated CD3 complex that is expressed on thymocytes and mature T cells.
  • Source
    Monoclonal Rat IgG2B Clone # 17A2
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    T cell hybridoma D1
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with BSA as a carrier protein.
  • Label
    Biotin
Applications
  •  
    Recommended
    Concentration
    Sample
  • Flow Cytometry
    2.5 µg/106 cells
    Mouse splenocytes
  • Immunohistochemistry
    Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
  • Immunoprecipitation
    Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
  • Immunocytochemistry
    8-25 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Immunocytochemistry
CD3 in Mouse Splenocytes. CD3 was detected in immersion fixed mouse splenocytes using Rat Anti-Mouse CD3 Biotinylated Monoclonal Antibody (Catalog # BAM4841) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Streptavidin (yellow; Catalog # NL999) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.
Preparation and Storage
  • Reconstitution
    Reconstitute at 0.5 mg/mL in sterile PBS.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD3
The T cell receptor (TCR)/CD3 complex consists of two variable antigen-recognition receptor chains (TCR-alpha/TCR-beta or TCR-gamma/TCR-delta) that are non-covalently linked to at least four different invariant chains: CD3 gamma, CD3 delta, CD3 epsilon, and the CD3 zeta chain that exists as either a homodimer or as a heterodimer with its splice-variant the CD3 eta chain. The CD3 complex functions to transduce intracellular signals during TCR antigen recognition. CD3 is expressed early in thymocyte development, and on a subset of NK cells. CD3 epsilon is one of at least three invariant proteins that associate with the variable antigen recognition chains of the T cell receptor and function in signal transduction.
  • References:
    1. Miescher, G.C. et al. (1989) Immunol. Lett. 23:113.
  • Entrez Gene IDs:
    916 (Human); 12503 (Mouse)
  • Alternate Names:
    CD3 antigen, delta subunit; CD3; CD3d antigen; CD3d antigen, delta polypeptide (TiT3 complex); CD3d molecule, delta (CD3-TCR complex); CD3-DELTA; CD3e antigen; CD3e antigen, epsilon polypeptide (TiT3 complex); CD3e molecule, epsilon (CD3-TCR complex); CD3-epsilon; CD3g antigen; CD3g antigen, gamma polypeptide (TiT3 complex); CD3g molecule, epsilon (CD3-TCR complex); CD3g molecule, gamma (CD3-TCR complex); CD3G; CD3-GAMMA; FLJ17620; FLJ17664; FLJ18683; FLJ79544; FLJ94613; MGC138597; T3DOKT3, delta chain; T3E; T-cell antigen receptor complex, epsilon subunit of T3; T-cell receptor T3 delta chain; T-cell surface antigen T3/Leu-4 epsilon chain; T-cell surface glycoprotein CD3 delta chain; T-cell surface glycoprotein CD3 epsilon chain; TCRE
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