Mouse CD40/TNFRSF5 Biotinylated Antibody
Mouse CD40/TNFRSF5 Biotinylated Antibody Summary
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Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD40 is a type I transmembrane glycoprotein belonging to the TNF receptor superfamily. The mature mCD40 consists of a 172 amino acid (aa) extracellular domain, a 22 aa transmembrane region and a 90 aa cytoplasmic domain (1). CD40 is expressed on B cells, follicular dendritic cells, dendritic cells, activated monocytes, macrophages, endothelial cells, vascular smooth muscle cells and several tumor cell lines (2). The extracellular domain has the cysteine-rich repeat regions, which are characteristic for many of the receptors of the TNF superfamily. Interaction of CD40 with its ligand, CD40L, leads to the aggregation of CD40 molecules, which in turn interact with cytoplasmic components to initiate signaling pathways. Early studies on the CD40-CD40L system revealed its role in humoral immunity. Interaction between CD40L on T cells and CD40 on B cells stimulated B cell proliferation and provided the signal for immunoglobulin isotype switching (3). Mutations in the CD40L gene, which resulted in a CD40L molecule unable to interact with CD40, are responsible for the hyper-IgM syndrome (4). Cross-linking of CD40 with antibodies or by binding to CD40L produces cell type-specific responses which include costimulation and induction of proliferation, induction of cytokine production, rescue from apoptosis, and upregulation of adhesion molecules (5). Some of the early events of intracellular signaling by the CD40-CD40L system include the association of the CD40 with TRAFs and the activation of various kinases (6‑8).
- Torres, R.M. and E.A. Clark (1992) J. Immunol. 148:620.
- Schonbeck, U. et al. (1997) J. Biol. Chem. 272:19569.
- Armitage, R.J. et al. (1993) J. Immunol. 150:3671.
- Callard, R.E. et al. (1993) Immunol. Today 14:559.
- Stout, R.D. and J. Suttles (1996) Immunol. Today 17:487.
- Pullen, S.S. et al. (1999) Biochemistry 38:10168.
- Faris, M. et al. (1994) J. Exp. Med. 179:1923.
- Hanissian, S.H. and R.S. Geha (1997) Immunity 6:379.
Citation for Mouse CD40/TNFRSF5 Biotinylated Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
1 Citation: Showing 1 - 1
Expression, purification, and functional analysis of an antigen-targeting fusion protein composed of CD40 ligand and the C-terminal fragment of ovalbumin
Authors: Y Shi, SA Halperin, SF Lee
Protein Expr. Purif., 2017;142(0):37-44.
Sample Types: Recombinant Protein
Applications: Western Blot
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