Mouse CD45.1 APC‑conjugated Antibody

FAB8374A has been discontinued. View all CD45.1 products.
  • Species Reactivity
  • Specificity
    Detects mouse cells expressing the CD45.1 allotype in flow cytometry. Clone A20 does not detect the mouse CD45.2 alloantigen.
  • Source
    Monoclonal Mouse IgG2A Clone # A20
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    SJL mouse thymocytes and splenocytes
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples

Detection of CD45.1 in Mouse Splenocytes by Flow Cytometry. SJL mouse splenocytes were stained with Mouse Anti-Mouse CD45.1 APC‑conjugated Monoclonal Antibody (Catalog # FAB8374A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.

Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD45.1
CD45, previously called LCA (Leukocyte Common Antigen), T200, or Ly5 in mice, is member C of the Class 1 (Receptor-like) Protein Tyrosine Phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1-3). CD45.1 is an alloantigen of CD45, expressed by Ly5.1 bearing mouse strains (RIII, SJL/J, STS/A, DA).
  • References:
    1. Anderson, J.N. et al. (2004) FASEB J. 18:8.
    2. Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
    3. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
  • Long Name:
    Cluster of Differentiation 45.1
  • Entrez Gene IDs:
    5788 (Human); 19264 (Mouse)
  • Alternate Names:
    B220; CD45 antigen; CD45.1; CD45R; EC; L-CA; LY5; Ly-5.1; protein tyrosine phosphatase, receptor type, C; receptor-type tyrosine-protein phosphatase C; T200 glycoprotein; T200 leukocyte common antigen; T200receptor type, c polypeptide
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