Mouse CD45.2 Alexa Fluor® 488-conjugated Antibody

  • Species Reactivity
  • Specificity
    Detects mouse cells expressing the CD45.2 allotype in flow cytometry. Clone 104 does not detect the mouse CD45.1 alloantigen.
  • Source
    Monoclonal Mouse IgG2A Clone # 104
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    B10.S mouse thymocytes and splenocytes
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
    Alexa Fluor 488
  • Flow Cytometry
    5 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of CD45.2 in BALB/c Mouse Splenocytes by Flow Cytometry. BALB/c mouse splenocytes were stained with Mouse Anti-Mouse CD45.2 Alexa Fluor® 488‑conjugated Monoclonal Antibody (Catalog # FAB8448G, filled histogram) or isotype control antibody (Catalog # IC003G, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD45.2
CD45, previously called LCA (Leukocyte Common Antigen), T200, or Ly5 in mice, is member C of the Class 1 (Receptor-like) Protein Tyrosine Phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1-3). CD45.2 is an alloantigen of CD45 expressed by Ly5.2+ mouse strains including: AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, and 129.
  • References:
    1. Anderson, J.N. et al. (2004) FASEB J. 18:8.
    2. Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
    3. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
  • Long Name:
    Cluster of Differentiation 45.2
  • Entrez Gene IDs:
    5788 (Human); 19264 (Mouse)
  • Alternate Names:
    B220; CD 45; cd45 antigen; CD45.2; CD45R; GP 180; GP180; Human homolog of severe combined immunodeficiency due to PTPRC deficiency; L CA; LCA; Leukocyte common antigen; LY 5; Ly5; Ly5.2; Ly-5.2; Protein tyrosine phosphatase receptor type c polypeptide; Protein tyrosine phosphatase receptor type C; PTPRC; RT7.2; SCID due to PTPRC deficiency; T200 glycoprotein; T200 leukocyte common antigen; T200
Related Research Areas
Isotype Controls
Description Application Cat# Citations Images  

Mouse IgG2A Alexa Fluor® 488-conjugated Antibody

Ctrl IC003G  
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....
Staining Reagents
Description Application Cat# Citations Images  

Flow Cytometry Staining Buffer (1X)

Flow FC001 3
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Mouse Lyse Buffer (10X)

Flow FC003 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Flow Cytometry Human Lyse Buffer (10X)

Flow FC002 1
View Sizes & Prices
Catalog# Size Price Stock Quantity
Loading Information....

Have you used Mouse CD45.2 Alexa Fluor® 488-conjugated Antibody?

Submit a review and receive a $25 amazon gift card if you include an image - $10 Amazon card for reviews without an image. Limited to verified customers in USA, Canada and Europe.

Order Details

Contact Information

Reconstitution Calculator

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Certificate of Analysis Lookup

The document you requested is not available online. Please enter the Catalog Number and Lot Number below to have a document emailed to you at the address provided

Certificate of Analysis Lookup

Research Areas of Interest
R&D Systems Guarantee

Associated Pathways


Customer Information