Mouse CD45.2 APC-conjugated Antibody

FAB8448A has been discontinued. View all CD45.2 products.
Datasheet / CoA / SDS
Product Details
  • Species Reactivity
  • Specificity
    Detects mouse cells expressing the CD45.2 allotype in flow cytometry. Clone 104 does not detect the mouse CD45.1 alloantigen.
  • Source
    Monoclonal Mouse IgG2A Clone # 104
  • Purification
    Protein A or G purified from hybridoma culture supernatant
  • Immunogen
    B10.S mouse thymocytes and splenocytes
  • Formulation
    Supplied in a saline solution containing BSA and Sodium Azide.
  • Label
Product Datasheets

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  • Flow Cytometry
    10 µL/106 cells
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Example
Detection of CD45.2 in BALB/c Mouse Splenocytes by Flow Cytometry. BALB/c mouse splenocytes were stained with Mouse Anti-Mouse CD45.2 APC‑conjugated Monoclonal Antibody (Catalog # FAB8448A, filled histogram) or isotype control antibody (Catalog # IC003A, open histogram). View our protocol for Staining Membrane-associated Proteins.
Preparation and Storage
  • Shipping
    The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
  • Stability & Storage
    Protect from light. Do not freeze.
    • 12 months from date of receipt, 2 to 8 °C as supplied.
Background: CD45.2
CD45, previously called LCA (Leukocyte Common Antigen), T200, or Ly5 in mice, is member C of the Class 1 (Receptor-like) Protein Tyrosine Phosphatase family (PTPRC) (1, 2). It is a variably glycosylated 180-220 kDa transmembrane protein that is abundantly expressed on all nucleated cells of hematopoietic origin (1-3). CD45.2 is an alloantigen of CD45 expressed by Ly5.2+ mouse strains including: AKR, BALB/c, CBA/Ca, CBA/J, C3H/He, C57BL, C57BR, C57L, C58, DBA/1, DBA/2, NZB, SWR, and 129.
  • References:
    1. Anderson, J.N. et al. (2004) FASEB J. 18:8.
    2. Streuli, M. et al. (1987) J. Exp. Med. 166:1548.
    3. Hermiston, M.L. et al. (2003) Annu. Rev. Immunol. 21:107.
  • Long Name:
    Cluster of Differentiation 45.2
  • Entrez Gene IDs:
    5788 (Human); 19264 (Mouse)
  • Alternate Names:
    B220; CD 45; cd45 antigen; CD45.2; CD45R; GP 180; GP180; Human homolog of severe combined immunodeficiency due to PTPRC deficiency; L CA; LCA; Leukocyte common antigen; LY 5; Ly5; Ly5.2; Ly-5.2; Protein tyrosine phosphatase receptor type c polypeptide; Protein tyrosine phosphatase receptor type C; PTPRC; RT7.2; SCID due to PTPRC deficiency; T200 glycoprotein; T200 leukocyte common antigen; T200
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