Mouse CD79A Antibody

  
  • Species Reactivity
    Mouse
  • Specificity
    Detects mouse CD79A in direct ELISAs and Western blots. In direct ELISAs, approximately 20% cross-reactivity with recombinant human CD79A is observed.
  • Source
    Polyclonal Sheep IgG
  • Purification
    Antigen Affinity-purified
  • Immunogen
    Chinese hamster ovary cell line CHO-derived recombinant mouse CD79A
    Leu29-Arg137
    Accession # P11911
  • Formulation
    Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied as a 0.2 µm filtered solution in PBS.
  • Label
    Unconjugated
Applications
  •  
    Recommended
    Concentration
    Sample
  • Western Blot
    1 µg/mL
    See below
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Data Examples
Detection of Mouse CD79A by Western Blot. Western blot shows lysates of A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse CD79A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6968) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD79A at approximately 32-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
  • Reconstitution
    Sterile PBS to a final concentration of 0.2 mg/mL.
  • Shipping
    The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
  • Stability & Storage
    Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
    • 12 months from date of receipt, -20 to -70 °C as supplied.
    • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
    • 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: CD79A

CD79A (also known as Mb-1, Ig alpha and B cell antigen receptor complex-associated protein alpha -chain) is a 30-40 kDa member of the Ig-Superfamily. It is expressed on B cells, and forms a covalent heterodimer with CD79B. This complex interacts noncovalently with membrane Ig, forming the B cell antigen receptor. Within this complex, membrane Ig detects antigen while CD79A:B initiates signaling. CD79A is essential for the differentiation of pre-B cells, and the pre-BCR regulates the surface expression of IL-7R. Mature mouse CD79A is a 192 amino acid (aa) type I transmembrane glycoprotein (aa 29-220). It contains an extracellular region (aa 29-137) with one C2-type Ig-like domain (aa 29-117), and an ITAM-containing cytoplasmic domain (aa 171-199). Mouse CD79A and CD79B share only 22% aa identity. Over aa 29-137, mouse CD79A shares 57% and 80% aa identity with human and rat CD79A, respectively.

  • Entrez Gene IDs:
    973 (Human); 12518 (Mouse); 681236 (Rat)
  • Alternate Names:
    CD79A antigen (immunoglobulin-associated alpha); CD79a antigen; CD79a molecule, immunoglobulin-associated alpha; CD79A; IGAB-cell antigen receptor complex-associated protein alpha chain; ig-alpha; MB-1 membrane glycoprotein; MB1; MB-1; Membrane-bound immunoglobulin-associated protein; Surface IgM-associated protein
Related Research Areas
Isotype Controls
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Secondary Antibodies
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Sheep IgG (H+L) PE-conjugated Antibody

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Sheep IgG VisUCyte HRP Polymer Antibody

IHC VC006  
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Sheep IgG (H+L) APC-conjugated Antibody

Flow F0127  
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Donkey Anti-Sheep IgG (H+L) Fluorescein-conjugated Antibody

Flow F0125 1
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Donkey Anti-Sheep IgG (H+L) PerCP-conjugated Antibody

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