Detection of Mouse CD79A by Western Blot. Western blot shows lysates of A20 mouse B cell lymphoma cell line. PVDF membrane was probed with 1 µg/mL of Sheep Anti-Mouse CD79A Antigen Affinity-purified Polyclonal Antibody (Catalog # AF6968) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CD79A at approximately 32-35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.
Preparation and Storage
Sterile PBS to a final concentration of 0.2 mg/mL.
The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below. *Small pack size (SP) is shipped with polar packs. Upon receipt, store it immediately at -20 to -70 °C
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.
CD79A (also known as Mb-1, Ig alpha and B cell antigen receptor complex-associated protein alpha -chain) is a 30-40 kDa member of the Ig-Superfamily. It is expressed on B cells, and forms a covalent heterodimer with CD79B. This complex interacts noncovalently with membrane Ig, forming the B cell antigen receptor. Within this complex, membrane Ig detects antigen while CD79A:B initiates signaling. CD79A is essential for the differentiation of pre-B cells, and the pre-BCR regulates the surface expression of IL-7R. Mature mouse CD79A is a 192 amino acid (aa) type I transmembrane glycoprotein (aa 29-220). It contains an extracellular region (aa 29-137) with one C2-type Ig-like domain (aa 29-117), and an ITAM-containing cytoplasmic domain (aa 171-199). Mouse CD79A and CD79B share only 22% aa identity. Over aa 29-137, mouse CD79A shares 57% and 80% aa identity with human and rat CD79A, respectively.
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